Novel auxin signaling components and pathways.

新型生长素信号传导成分和途径。

• 批准号: 10797344
• 负责人: MARK A ESTELLE
• 金额: $ 3.86万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797344
• 关键词: Auxins; Cell physiology; Cullin Proteins; Cytoplasm; Defect; Disease; Family; Funding; Genes; Genetic; Growth; Growth and Development function; Hormones; Hybrids; Luciferases; Malignant Neoplasms; Measurement; Mediating; Molecular Chaperones; Organ; Pathway interactions; Plant Growth Regulators; Process; Proteins; Reader; Role; Signal Pathway; Signal Transduction; Specificity; Technology; Transcription Repressor; Transcriptional Regulation; Ubiquitin; cell growth regulation; cell type; gene regulatory network; high throughput screening; instrument; member; multicatalytic endopeptidase complex; non-genomic; novel; plant growth/development; proteostasis; receptor; response; synergism; ubiquitin-protein ligase; virtual

Project Summary The plant hormone auxin is involved in virtually all aspects of plant growth and development. Over the years we and others have demonstrated that auxin acts by stimulating the degradation of a family of transcriptional repressors called the Aux/IAA proteins, through the action of the ubiquitin protein ligase SCFTIR1/AFB. Although this basic pathway is well established, we still know very little about how auxin regulates plant growth and development. Strikingly, auxin-regulated genes differ dramatically between cell types and organs, consistent with the diverse roles of the hormone. The basis for this specificity is unknown. Similarly, the activities of the Aux/IAA and ARF proteins are poorly characterized and the gene regulatory networks (GRNs) that mediate various auxin-regulated growth processes have not been defined. Finally, we know little about how auxin signaling is integrated with other environmental and genetic signaling pathways. Recently we discovered that one member of the TIR1/AFB family of auxin co-receptors, AFB1, has a key role in a non-genomic rapid auxin response in the root. This was a surprising result because the TIR1/AFB proteins are known to function in transcriptional regulation. We also demonstrated that AFB1 is highly enriched in the cytoplasm and propose that it is this feature that leads to its specialized role in the rapid response. In this proposal, we request funds to purchase a Agilent Technologies Synergy H1 Hybrid Multi-Mode Microplate Reader. This instrument is ideally suited to high through-put assays of auxin response in Physcomitrellum and measurements of Luciferase levels.

项目概要 植物激素生长素几乎参与植物生长和发育的所有方面。超过 多年来，我们和其他人已经证明，生长素通过刺激一个家族的退化来发挥作用。 通过泛素蛋白连接酶的作用，称为 Aux/IAA 蛋白的转录抑制因子 SCFTIR1/AFB。尽管这一基本途径已被充分确立，但我们对生长素如何调节仍知之甚少。 植物生长和发育。引人注目的是，生长素调节基因在细胞类型和细胞类型之间存在显着差异。 器官，与激素的不同作用一致。这种特异性的基础尚不清楚。相似地， Aux/IAA 和 ARF 蛋白的活性尚不清楚，基因调控网络 介导各种生长素调节的生长过程的（GRN）尚未被定义。最后，我们所知甚少 关于生长素信号如何与其他环境和遗传信号通路整合。 最近我们发现生长素辅助受体 TIR1/AFB 家族的一个成员 AFB1 具有一个关键的 在根中非基因组快速生长素反应中的作用。这是一个令人惊讶的结果，因为 TIR1/AFB 已知蛋白质在转录调节中发挥作用。我们还证明了 AFB1 具有高度 富集于细胞质中，并提出正是这一特征导致其在快速 回复。 在此提案中，我们请求资金购买 Agilent Technologies Synergy H1 混合多模 酶标仪。该仪器非常适合生长素反应的高通量测定 立碗藻和荧光素酶水平的测量。

项目成果

Heterologous expression of a lycophyte protein enhances angiosperm seedling vigor.

石松蛋白的异源表达增强被子植物幼苗活力。

• DOI: 10.1242/dev.200917
• 发表时间: 2022
• 期刊: Development (Cambridge, England)
• 作者: Koh,SamuelWH;Diaz-Ardila,HaroldNicholay;Bascom,CarlisleS;Berenguer,Eduardo;Ingram,Gwyneth;Estelle,Mark;Hardtke,ChristianS
• 通讯作者: Hardtke,ChristianS