Administrative supplement to purchase a MerMade 4 oligonucleotide synthesizer for the large-scale production of modified RNA substrates

购买 MerMade 4 寡核苷酸合成仪用于大规模生产修饰 RNA 底物的行政补充

• 批准号: 10797873
• 负责人: Jeffrey Scott Mugridge
• 金额: $ 9.51万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797873
• 关键词: Acceleration; Administrative Supplement; Binding; Biological Assay; Biophysics; Crystallography; Dioxygenases; Enzyme Interaction; Enzymes; Family; Funding; Goals; Human; Iron; Malignant Neoplasms; Messenger RNA; Mole the mammal; Molecular; Oligonucleotides; Parents; Play; Production; Proteins; RNA; RNA methylation; Reagent; Regulation; Request for Proposals; Research; Resolution; United States National Institutes of Health; demethylation; experimental study; large scale production; nucleobase; screening

ABSTRACT: Our NIH R35 ESI MIRA entitled ‘Selectivity and regulation of mRNA demethylation by iron- dependent dioxygenases’ focuses on understanding the structural and molecular determinants of AlkBH-family RNA demethylase enzymes that play key roles in RNA function and some human cancers. A critical challenge to carrying out some of the proposed structural experiments has been in obtaining sufficient quantities of modified RNA oligonucleotide substrates for crystallographic screening and biophysical binding assays. This administrative supplement requests funds to purchase an LGC MerMade 4 oligonucleotide synthesizer that will transform and greatly accelerate our ability to access key modified RNA oligonucleotide reagents to support multiple goals of our MIRA projects.

抽象的： 我们的NIH R35 ESI MIRA标题为“通过铁对mRNA脱甲基化的选择性和调节 依赖性二氧酶的重点是理解结构和分子决定者 在RNA功能中起关键作用和某些人 癌症。进行一些提出的结构实验的关键挑战具有 我们正在获得足够数量的改性RNA寡核苷酸底物 晶体学筛选和生物物理结合测定法。这种行政补充 请求资金购买LGC Mermade 4寡核苷酸合成器，该合成器将转换 并极大地加速了我们访问密钥修饰的RNA寡核苷酸试剂的能力 MIRA项目的多个目标。