Behavior Core

行为核心

基本信息

批准号：
10705994
负责人：
Adam Christopher Mar
金额：
$ 57.84万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-15 至 2028-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10705994
关键词：
3-Dimensional Affect Agonistic Behavior Animal Behavior Animal Welfare Animals BRAIN initiative Behavior Behavior assessment Behavior monitoring Behavioral Biological Assay Breeding Collaborations Communication Complex Computer Vision Systems Computer software Computers Core Facility Custom Darkness Data Data Analyses Data Collection Data Science Core Data Set Decision Making Detection Development Ensure Environment Ethology Experimental Designs Funding Genetic Home Hour Housing Image Individual Infrastructure Investments Laboratories Light Machine Learning Measures Methods Modality Molecular Monitor Mus Neurosciences Oxytocin Parents Partner in relationship Postdoctoral Fellow Posture Protocols documentation Quantitative Evaluations Research Resources Rodent Scientist Social Development Social Environment Social Hierarchy Social Interaction Spatial Behavior Specific qualifier value Standardization System Techniques Testing Training Transgenic Mice Validation Vendor Work affiliative behavior analysis pipeline analytical tool behavior test commune data pipeline data streams deep learning design experience facility renovation flexibility improved information processing innovation learning network medical schools member neural neural circuit neuroregulation next generation novel optogenetics parallel processing predictive modeling social success tool touchscreen training project

项目摘要

Project Summary: Behavior Core Long-term (days to months) and continuous (24 hours/day, 7 days/week) behavioral monitoring of mouse socio- spatial behavior is fundamental to this BRAIN Initiative proposal on “Oxytocin Modulation of Neural Circuit Function and Behavior”. Each Project requires behavioral assessment of mice in home cages or in larger more complex environments, often longitudinally and in conjunction with neural recordings, optogenetics, or other manipulations of cellular and network-level activity. A number of recent technical advances across levels now enable a deeper understanding of neuromodulation on flexible information processing and behavior in mice. Some of these innovations have been pioneered by our laboratories and the Projects/Cores of the Oxytocin U19 in the previous funding cycle. Specifically, it is now possible- if not routine- to apply advanced machine learning and computer vision methods for quantitative behavioral analysis of animal poses and trajectories from video data. This includes video tracking of multiple animals interacting, from different camera angles, across the light/dark cycle, and with simultaneous neural recordings. Over the last five years we have developed hardware, software, and other infrastructure to perform this continuous behavioral monitoring in our facility. Our successes have led to considerable investment in space and computer storage for this facility by NYU Langone. We now capitalize on the development of this facility and behavioral monitoring system to support the next-generation studies of multi-animal behavior and modulation of neural circuit function by the Oxytocin U19 teams. Aim 1 is to improve our capacity for behavioral home cage monitoring, both in terms of number of animals monitored per cage and the pipeline for data collection and analysis. Aim 2 is to develop more complex housing environments for studies of socio-spatial behavior and decision-making in mouse colonies of small to moderate sizes. Aim 3 is to provide a suite of additional social, spatial and other oxytocin-related testing available to Project teams and assisted by Behavior Core staff, including breeding and housing the set of transgenic mouse lines required for this U19 proposal.

项目摘要：行为核心对小鼠社会行为进行长期（数天至数月）和连续（24小时/天，7天/周）行为监测空间行为是 BRAIN Initiative 关于“神经回路催产素调节”提案的基础每个项目都需要对家庭笼子或更大的笼子中的小鼠进行行为评估。复杂的环境，通常是纵向的并与神经记录、光遗传学或其他相结合细胞和网络级活动的操纵现在有一些跨级别的最新技术进步。使人们能够更深入地了解神经调节对小鼠灵活信息处理和行为的影响。其中一些创新是由我们的实验室和催产素 U19 项目/核心开创的具体来说，现在可以应用先进的机器学习，即使不是常规的。和计算机视觉方法，用于对视频中的动物姿势和轨迹进行定量行为分析这包括从不同摄像机角度对多个动物进行交互的视频跟踪。光/暗循环，并通过同步神经记录在过去五年中我们开发了硬件，软件和其他基础设施在我们的设施中执行这种持续的行为监控我们的成功。纽约大学朗格尼分校对该设施的空间和计算机存储进行了大量投资。我们现在利用该设施和行为监控系统的开发来支持催产素 U19 对多种动物行为和神经回路功能调节的下一代研究团队的目标 1 是提高我们对家庭笼子的行为监测的能力，无论是在动物数量方面。监控每个笼子和管道以进行数据收集和分析。目标 2 是开发更复杂的住房。研究中小型小鼠群体的社会空间行为和决策的环境目标 3 是为项目提供一套额外的社会、空间和其他催产素相关测试。团队并在行为核心人员的协助下，包括培育和饲养一组转基因小鼠品系此 U19 提案所需。