A transgenic songbird to image brain premotor sequence

转基因鸣禽对大脑前运动序列进行成像

基本信息

批准号：
9034727
负责人：
CARLOS LOIS
金额：
$ 26.53万
依托单位：
CALIFORNIA INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-15 至 2017-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9034727
关键词：
Animal Model Animals Behavior Behavioral Biological Models Birds Brain Brain imaging Cell Nucleus Cells Clinical Complex Computers Data Development Disease Exhibits Generations Genetic Goals Head Image Individual Injection of therapeutic agent Injury Label Laboratories Lead Learning Mammals Maps Measurable Methods Modeling Monitor Motor Movement Movement Disorders Muscle Neurons Neurosciences Parkinson Disease Pathway interactions Pattern Prosthesis Reporter Resolution Sampling Science Sensory Signal Transduction Songbirds Stereotyped Behavior Stereotyping Stroke Synapsins System Techniques Training Transgenes Transgenic Animals Transgenic Organisms Ubiquitin C Variant Viral Viral Vector Virus brain machine interface cell type central nervous system injury effective therapy fluorescence microscope functional restoration improved insight learned behavior millisecond motor control motor deficit motor learning motor skill learning neuromechanism neuronal circuitry neuronal patterning next generation promoter public health relevance relating to nervous system response to injury sequence learning stereotypy tool zebra finch

项目摘要

DESCRIPTION (provided by applicant): The neural mechanisms underlying learned motor behaviors are one of the least understood aspects of neuroscience, yet treatment of motor deficits after injury or disease is a significant clinical need. A better understanding of the organization of motor control could lead to effective treatments that provide enhancements of motor plasticity, or brain machine interfaces for functional restoration. However, at the cellular level, little is known about how brain circuits are able to learn and reproduce temporal sequences. One the greatest challenges involved in investigating natural motor control is the lack of repeatability of the learned motor skill. To image neuronal activity during the execution o a highly complex, yet stereotyped, behavior we propose to develop a transgenic songbird expressing the genetically-encoded Ca++ indicator GCaMP6. We will use the zebra finch, a species that learns to produce an extremely stereotyped song pattern, with strikingly small trial-to trial variations. Thus, this system provides a rare model from which to tightly correlate functional changes in neuronal activity to specific and readily measurable behavioral changes. Uncovering principles relating motor behavior and cell type specific circuit activity will provide key insights governing sensory-motor learning, adaptive plasticity in response to injury, and also provide insights for the development of next generation brain-machine interfaces.

描述（由申请人提供）：习得运动行为背后的神经机制是神经科学中最不为人所知的方面之一，但损伤或疾病后运动缺陷的治疗是一个重要的临床需求，更好地理解运动控制的组织可能会导致这种需求。然而，在细胞水平上，人们对大脑回路如何学习和复制时间序列知之甚少，这是研究自然运动的最大挑战之一。控制是缺乏为了对执行高度复杂但刻板的行为过程中的神经活动进行成像，我们建议开发一种表达基因编码的 Ca++ 指示剂 GCaMP6 的转基因鸣禽。产生极其刻板的歌曲模式，并且试验之间的变化非常小，因此，该系统提供了一种罕见的模型，可以将神经活动的功能变化与特定且易于测量的行为变化紧密相关。与运动行为和细胞类型特定电路活动相关的原理将为控制感觉运动学习、响应损伤的适应性可塑性提供关键见解，并为下一代脑机接口的开发提供见解。