Animal, Imaging, and Cytometry Core

动物、成像和细胞计数核心

基本信息

批准号：
8792578
负责人：
Kewal Asosingh
金额：
$ 18.24万
依托单位：
CLEVELAND CLINIC LERNER COM-CWRU
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-04-15 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8792578
关键词：
Abbreviations Allergens Allergic Animal Experiments Animals Asthma Biological Models Biology Breeding Cells Complex Cyclic AMP Cytometry Data Databases Electroporation Emission-Computed Tomography Figs - dietary Functional Imaging Gene Proteins Generations Genes Histologic IACUC Image Image Cytometry Knock-out Knockout Mice Lung Maintenance Measurement Mitochondria Modeling Mouse Strains Mus NOS1 gene NOS2A gene NOS3 gene Nitric Oxide Nuclear Ovalbumin Oxidoreductase Pathway interactions Performance Phosphatidylinositols Phosphorylation Phosphotransferases Photons Positron-Emission Tomography Procedures Process Process Measure Pulmonary Inflammation Pyroglyphidae Radiation Radio Radioactive Receptor Signaling Research Safety Sampling Services Smooth Muscle Soluble Guanylate Cyclase Structure of parenchyma of lung Tissues airway hyperresponsiveness airway inflammation animal imaging arginase beta-adrenergic receptor cost in vivo innovation instrument knockout animal molecular imaging programs pyroglyphid research study success tissue processing

项目摘要

ABSTRACT - CORE D The Animal Imaging and Cytometry Core will support all Projects of the Program by providing key services, including: generation, breeding, and maintenance of genetically modified mouse colonies, murine asthma models (ovalbumin allergen, dust mite, and intratracheal IL-25 and in vivo NOS electroportion), mouse airway hyper-reactivity measures, processing and histological analyses of mouse lung tissues, murine data repository, in vivo-molecular imaging by Positron Emission Tomography (PET) and Single Photon Emission Computerized Tomography (SPECT) for iNOS activity and adherent cell cytometric analyses for intracellular probes. All required instruments and expertise are found in the Core. The Core expedites results and reduces cost of Projects through the use of shared model systems. New model systems and functional imaging increase overall innovation of the Program.

摘要 - 核心 D 动物成像和细胞计数核心将通过提供关键服务来支持该计划的所有项目，包括：转基因小鼠群体的产生、繁殖和维持、小鼠哮喘模型（卵清蛋白过敏原、尘螨、气管内 IL-25 和体内 NOS 电击）、小鼠气道小鼠肺组织的高反应性测量、处理和组织学分析、小鼠数据存储库、通过正电子发射断层扫描 (PET) 和单光子发射计算机化进行体内分子成像用于 iNOS 活性的断层扫描 (SPECT) 和用于细胞内探针的贴壁细胞计数分析。全部所需的工具和专业知识可以在核心中找到。核心加速结果并降低成本通过使用共享模型系统进行项目。新模型系统和功能成像增加方案整体创新。