Functional Genomics Core

功能基因组学核心

基本信息

批准号：
10704366
负责人：
Leah Claire Kottyan
金额：
$ 16.05万
依托单位：
CINCINNATI CHILDRENS HOSP MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2028-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10704366
关键词：
ATAC-seq Acceleration Affinity Alleles Animal Model Autoimmune Automobile Driving Binding Biological Assay Biological Process Board Certification Cells ChIP-seq Chromatin Chromatin Loop Clinical Clinical Management Clinical assessments Collaborations Communities Complex Consultations DNA DNA Sequence DNA sequencing Development Disease Disease model Doctor of Philosophy Electrophoretic Mobility Shift Assay Enhancers Epidemiology Etiology Family Genes Genetic Genetic Risk Genetic Transcription Genetic study Genomic approach Genomics Genotype Histones Immune Immune System Diseases Immunoprecipitation Individual Inflammatory Infrastructure Investigation Libraries Luciferases Measures Medical center Molecular Molecular Biology Pathogenesis Pathologist Pathology Patients Pediatric Hospitals Peer Review Phenotype Physicians Plasmids Population Precipitation Preparation Productivity Proteins Protocols documentation Publications RNA Reagent Regulation Regulator Genes Reporter Research Research Design Research Personnel Research Project Grants Resources Rheumatism Role Running Scientist Services System Technical Expertise Technology Time Tissue Sample Tissues Transfection Translations autoinflammatory base chromatin immunoprecipitation cost deep sequencing experimental study functional genomics genetic association genetic regulatory protein genetic variant genome-wide genomic data histone modification innovation insight molecular diagnostics novel therapeutic intervention pediatric rheumatic diseases polygenic risk score promoter risk variant screening single-cell RNA sequencing tissue preparation tool transcriptome sequencing transcriptomics

项目摘要

Project Summary/Abstract – Functional Genomics Core (FGC) The Functional Genomics Core (FGC) will provide reagents, strategic, and technical expertise required for efficient investigation of genetic variants likely to be mechanistically important in pediatric rheumatic and inflammatory diseases. In doing so, the Functional Genomics Core will enhance research productivity and efficiency by effectively reducing the “start-up” time required to implement these complex experimental systems, thus reducing costs and effort required and allowing for better study designs and higher quality studies than if investigators were to individually establish this technology. Further, this core will provide access to state-of-the- art technology without requiring the users to become experts in these technologies. The Functional Genomics Core will be a practical vehicle for the translation of basic advances to clinical disease applications and their very important insights into pathogenesis. We will specifically provide consultation, collaboration, hands-on technical expertise, and starter reagents to the research community to perform and analyze functional genomic experiments (Aim1) and identify genotype-dependent gene regulatory mechanisms (Aim 2) to assess the biological function of genetic variants associated with of autoimmune, autoinflammatory, and immune-related rheumatic pediatric diseases. We expect the Functional Genomics Core to be a hub of innovation for the research community, connecting scientists with the tools and expertise to take their research projects to the next level and to accelerate the discovery of the molecular mechanisms that are responsible for the genetic associations observed.

项目摘要/摘要 - 功能基因组学核心（FGC）功能基因组学核心（FGC）将提供试剂，战略和技术专长遗传变异的效力投资可能在小儿风湿性和炎症性疾病。通过有效减少实施这些复杂的实验系统所需的“启动”时间来效率，因此，降低了所需的成本和精力，并允许更好地研究设计和更高的研究调查人员将进一步建立这项技术。艺术技术要求用户成为这些技术的专家核心将是将基本进步转化为临床疾病应用及其非常实用的工具对发病机理的重要见解。专业知识和研究界的起动试剂执行和分析功能基因组实验（AIM1）并确定基因型依赖性基因调节机制（AIM 2）以评估您与自身免疫性，自身炎症和免疫相关的遗传变异的生物学功能风湿病的儿科疾病。社区，将科学家与工具和专业知识联系到他们的研究项目的一个新水平，并且加速负责遗传关联的分子机制观察到。