Cannabis Vapor Exposure Impacts Reproduction

大麻蒸气暴露影响繁殖

• 批准号: 10703501
• 负责人: KANAKO HAYASHI
• 金额: $ 18.48万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10703501
• 关键词: Abnormal placentation; Adult; Adverse effects; Affect; Age; American; Apoptosis; Cannabidiol; Cannabis; Chronic; Consumption; Control Groups; DNA; DNA Damage; DNA Methylation; DNA Modification Methylases; Data; Defect; Discipline of Nursing; Drug usage; Epigenetic Process; Estrous Cycle; Ethics; Exhibits; Exposure to; Female; Fertility; First Pregnancy Trimester; Generations; Germ Cells; Health; Human; Inherited; Knowledge; Menstruation; Mus; Neonatal; Outcome; Ovary; Partner in relationship; Paternal Exposure; Pharmaceutical Preparations; Phenotype; Plants; Plasma; Population; Pregnancy; Pregnant Women; Premature Birth; Psychoses; Psychotropic Drugs; Reporting; Reproduction; Risk; Socioeconomic Factors; Sperm Count Procedure; Sperm Motility; Standardization; Surveys; Testing; Testis; Tetrahydrocannabinol; Toxicology; United States; Vagina; bisphenol A; design; histone methyltransferase; histone modification; in utero; insight; male; marijuana legalization; marijuana use; marijuana user; methylome; offspring; postnatal; pregnant; reproductive; reproductive function; sperm cell; steroid hormone; stillbirth; transcriptome; transmission process; vapor

Project Summary Cannabis is considered the most widely used psychoactive drug in the United States and all over the world. In 2020, the National Survey on Drug Use and Health (NSDUH) has reported that over 48 million Americans (~16% of the population) ages 12 and older used cannabis in the past year. This number has increased due to the expanding legalization of cannabis consumption in the US. Higher potency strains of cannabis plants have emerged over the past two decades. Unsurprisingly, adverse health outcomes associated with cannabis consumption have also been increasing. The proposed study will provide the first impact, which is the transgenerational effects of in utero and nursing cannabis vapor exposure on phenotypic defects of male and female reproductive functions. Because cannabis is a psychoactive drug, chronic or frequent use of cannabis is associated with a variety of adverse health outcomes. However, little is known about its effects on reproduction, especially the long-term consequences of cannabis on offspring and subsequent generations. To date, no systematic studies examining reproductive phenotypic defects and their associated mechanisms caused by cannabis have been performed. In addition, it is entirely unknown whether the adverse effects of cannabis on reproduction are sustained via germline transmission to subsequent generations. Therefore, this application proposes as a first step to examine the transgenerational effects of cannabis vapor exposure on male and female reproductive functions. We will characterize how cannabis vapor exposure during pregnancy and nursing affects male and female reproductive parameters in neonatal and adult testes and ovaries to understand paternally or maternally transmitted alterations in male or female germ cells. Because at least 7% of pregnant women use cannabis throughout pregnancy (~12% during the first trimester and 5.3% daily users), an appreciation of the impact of cannabis use on reproductive functions in subsequent offspring is crucial.

项目摘要 大麻被认为是美国和世界各地最广泛使用的精神活性药物。在 2020年，全国毒品使用与健康调查（NSDUH）报告说，超过4800万美国人 （约占人口的16％）12岁及以上的年龄在过去一年中使用了大麻。由于这个数字增加了 美国大麻消费的合法化不断扩大。大麻植物的效力较高 在过去的二十年中出现了。毫不奇怪，与大麻相关的不利健康结果 消费也在增加。拟议的研究将提供第一个影响，即 子宫和护理大麻蒸气暴露对男性和 女性生殖功能。因为大麻是一种精神活性药物，所以长期或频繁使用大麻 与各种不良健康结果有关。但是，关于其对其对的影响知之甚少 繁殖，尤其是大麻对后代和后代的长期后果。到 日期，没有系统的研究检查生殖表型缺陷及其相关机制 由大麻引起的。另外，完全未知是否 繁殖的大麻通过生殖线传播到随后的几代。因此，这个 应用建议是检查大麻蒸气暴露对跨代作用的第一步 男性和女性生殖功能。我们将表征怀孕期间大麻蒸气的暴露 护理会影响新生儿和成人睾丸和卵巢中的男性和女性生殖参数 了解男性或雌性生殖细胞的父亲或母体传播改变。因为至少7％ 在整个怀孕期间，孕妇都使用大麻（在孕期约12％，每天5.3％的用户）， 对大麻使用对随后后代生殖功能的影响的欣赏至关重要。