Senescence tissue mapping and SASP Atlas for human somatic and reproductive tissues

人类体细胞和生殖组织的衰老组织图谱和 SASP 图谱

• 批准号: 10684947
• 负责人: Malene Hansen
• 金额: $ 51.51万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10684947
• 关键词: Adherence; Age; Aging; Applications Grants; Artificial Intelligence; Atlases; Award; Biological; Biological Markers; Breast; Cell Aging; Cells; Collaborations; Communication; Data; Data Analyses; Data Coordinating Center; Development; Drug or chemical Tissue Distribution; Ensure; Funding; Gene Expression Profile; Goals; Guidelines; Human; Human Resources; Knowledge; Leadership; Mammary Gland Parenchyma; Maps; Ovary; Pilot Projects; Policies; Procedures; Proteomics; Protocols documentation; Quality Control; Reporting; Reproducibility; Research; Research Activity; Research Personnel; Resources; Scientist; Site; Skeletal Muscle; Specimen; Technology; Tissue Banks; Tissues; Training; Travel; United States National Institutes of Health; Validation; Visit; Work; analysis pipeline; candidate marker; data access; data acquisition; data analysis pipeline; data quality; data resource; data sharing; data submission; design; experimental study; frailty; human tissue; improved; instrumentation; knowledge base; meetings; member; muscle aging; new technology; novel; reproductive; senescence; skills; success; synergism; technology development; tissue mapping; tool; transcriptome sequencing; transcriptomics

ADMINISTRATIVE CORE - PROJECT SUMMARY This Buck Institute Tissue Mapping Center (TMC) grant application proposes to map senescent cells in three human somatic and reproductive tissues, i.e., ovaries, breast tissue, and skeletal muscle. It is crucial to provide tissue maps of cellular senescence as well as a SASP Atlas of for these human tissues as (i) ovaries are one of the first tissues to age, (ii) breast tissue has been shown to rapidly age and display high SASP gene expression signatures in previous RNA Seq experiments, and (iii) muscle aging typically leads to impacted mobility or frailty. The Admin core will oversee a leadership plan, a tissue-mapping coordination plan and a network coordination plan to generate senescence tissue maps as part of the SenNet Consortium. The Admin Core will oversee that data sharing pipelines within the TMC and with the SenNet network will be implemented and improved. The collaboration between the Admin Core with the 3 other scientific Buck Institute TMC cores, the Biospecimen Core, Biological Analysis Core and Data Analysis Core, will enable a streamlined tissue collection, data acquisition and data analysis pipeline, in order to generate and prioritize senescence biomarkers expressed in the somatic and reproductive tissues. Regular discussions and meetings between the Admin Core leaders and leaders of the other scientific cores will allow efficient information exchange between all stakeholders. The Admin Core in collaboration with the Data Analysis Core will develop and implement plans for submission of data, protocols and resources to the SenNet Consortium and its data coordination center. The Admin Core will ensure that any data submitted to the consortium complies with network-wide standard operating procedures and SenNet policies. Collaborations with other TMC centers will enable comparisons of senescence markers between tissues, and applying different technologies that are available at different TMC centers for tissue mapping of multiple tissues, as the Admin Center will encourage all TMC centers to work together and to engage in cross-training of technological skill-sets, use of novel instrumentations for scientists between centers, and expanding intellectual knowledge about cellular senescence. Throughout the award period novel technological advancements will be implemented more broadly into TMC workflows and tissue biological analysis pipelines as they become available for network use. The Admin Core will coordinate such implementation in collaboration with other Technology Development Centers and other TMC centers. In summary, the Admin Core will coordinate all efforts of the TMC and build upon the existing strong scientific expertise in cellular senescence research at the Buck Institute to establish a new Senescence Tissue Mapping Atlas resource focusing on human ovaries, breast tissue, and skeletal muscle.

行政核心 - 项目摘要 这个Buck Institute组织映射中心（TMC）授予申请提议将三个细胞映射 人体细胞和生殖组织，即卵巢，乳腺组织和骨骼肌。提供的至关重要 这些人体组织的细胞衰老的组织图以及SASP地图 （ii）乳腺组织已显示出迅速年龄并显示出高SASP基因表达 以前的RNA SEQ实验和（iii）肌肉衰老的签名通常会导致迁移率或脆弱性。 管理员核心将监督领导计划，组织映射协调计划和网络协调 计划作为Sennet财团的一部分生成衰老组织图。管理员核心将监督 TMC和Sennet网络内的数据共享管道将被实施和改进。这 管理员核心与其他3个科学雄鹿研究所TMC核心之间的合作 核心，生物分析核心和数据分析核心将使精简组织收集，数据 获取和数据分析管道，以生成和优先考虑在 体细胞和生殖组织。管理员核心领导者与会议的定期讨论和会议 其他科学核心的领导者将允许所有利益相关者之间有效的信息交流。这 管理员核心与数据分析合作将制定并实施提交计划 向Sennet联盟及其数据协调中心提供的数据，协议和资源。管理员核心将 确保提交给财团的任何数据都符合网络范围的标准操作程序 和Sennet政策。与其他TMC中心的合作将启用衰老标记的比较 在组织之间，以及在不同TMC中心使用的不同技术 绘制多个组织，因为管理中心将鼓励所有TMC中心一起工作并参与 在技​​术技能集的交叉培训中，为中心之间的科学家使用新颖的仪器， 扩大有关细胞衰老的智力知识。在整个奖励期间小说技术 进步将在TMC工作流程和组织生物分析管道中更广泛地实施 它们可用于网络使用。管理员核心将协调此类实施 与其他技术开发中心和其他TMC中心。 总而言之，管理员核心将协调TMC的所有努力，并建立在现有的强大科学的基础上 Buck研究所的细胞衰老研究专业知识，以建立新的衰老组织映射 集中于人类卵巢，乳腺组织和骨骼肌的Atlas资源。