Endothelial Instruction of Macrophage Fate in Inflammatory Lung Injury

炎症性肺损伤中巨噬细胞命运的内皮指令

基本信息

批准号：
10701931
负责人：
Jalees Rehman
金额：
$ 42.8万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-20 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10701931
关键词：
ATP Citrate (pro-S)-Lyase Acetyl Coenzyme A Acute Lung Injury Adult Alveolar Macrophages Anti-Inflammatory Agents Binding Blood Vessels Cell Line Cell Nucleus Cells Characteristics Collaborations Complex Data Endothelial Cells Endothelium Enzymes Epigenetic Process G-Protein-Coupled Receptors Generations Genetic Transcription Histone Acetylation Homeostasis Human ITGAM gene Image Immune In Vitro Individual Inflammation Inflammatory Injury Instruction Leucine-Rich Repeat Licensing Life Lipoprotein Receptor LoxP-flanked allele Lung Macrophage Measures Mediating Metabolic Metabolism Mitochondria Modeling Modification Mus NADH Patients Phenotype Population Process Proteins Regulation Resolution Respiration Role Signal Transduction Testing Tissues Transcription Coactivator Transcriptional Activation Transcriptional Regulation WNT Signaling Pathway beta catenin cell type cofactor epigenetic regulation epigenome high resolution imaging in vivo interstitial lung injury metabolomics migration mitochondrial metabolism monocyte mouse model optogenetics prenatal programs receptor response to injury single-cell RNA sequencing synthetic biology tissue injury tissue repair transcriptomics

项目摘要

PROJECT SUMMARY/ABSTRACT Studies have shown much broader roles for macrophages in responding to inflammation and tissue injury than previously recognized, such as promoting inflammation resolution, tissue repair and restoring tissue homeostasis and this recognition of macrophage plasticity has shifted the focus away from the simple notion of M1 and M2 dichotomies. Project 3 focuses on delineating the signals mediating monocyte transition to tissue macrophages and their potentially essential role in tissue repair in acute lung injury (ALI). As the lung endothelium is the entry point of the monocytes transmigrating into tissue, endothelial cells (ECs) may modify the downstream macrophage phenotype, and thus the interaction between cells may be of great consequence. Project 3 will delineate mechanisms of generation of how ECs instruct macrophages and their role in resolving ALI. We will address fundamental questions including: What are the EC signals mediating transition to the reparative macrophage populations? What signals in macrophages in turn convert the cells to repair tissue? What are the epigenetic and transcriptomic features of these phenotype-shifted macrophages? In Project 3 we will test the hypothesis that the lung endothelium via Wnt signaling mediates macrophage phenotype transition through modifying mitochondrial metabolism and epigenome that initiates specific transcriptional programs. In Aim 1, we will define the role of endothelial Wnt signaling in regulating the differentiation of monocytes to macrophages in lungs. Here we will determine the role of the Wnt signaling regulator Rspondin 3 (Rspo3) derived from ECs in signaling the transition of monocytes to lung macrophages. In Aim 2, we will determine the role of metabolic reprogramming and epigenetic modifications of macrophages in mediating phenotype transition and thereby reducing the extent of lung injury as well as promoting its resolution.

项目概要/摘要研究表明，巨噬细胞在应对炎症和组织损伤方面的作用比先前认识到的，例如促进炎症消退、组织修复和恢复组织稳态这种对巨噬细胞可塑性的认识将焦点从 M1 和 M2 的简单概念转移开二分法。项目 3 重点描述介导单核细胞转变为组织巨噬细胞的信号和它们在急性肺损伤（ALI）的组织修复中具有潜在的重要作用。由于肺内皮是单核细胞迁移到组织中，内皮细胞（EC）可能会改变下游巨噬细胞表型，因此细胞之间的相互作用可能产生重大影响。项目3将描绘 ECs 指导巨噬细胞的产生机制及其在解决 ALI 中的作用。我们将解决基本问题包括：介导向修复性巨噬细胞转变的 EC 信号是什么人口？巨噬细胞中的哪些信号反过来将细胞转化为修复组织？什么是表观遗传和这些表型转变的巨噬细胞的转录组特征？在项目 3 中，我们将测试以下假设：肺内皮通过 Wnt 信号传导通过修饰线粒体介导巨噬细胞表型转变启动特定转录程序的代谢和表观基因组。在目标 1 中，我们将定义以下角色：内皮 Wnt 信号传导调节肺中单核细胞向巨噬细胞的分化。在这里我们将确定源自 EC 的 Wnt 信号调节因子 Rspondin 3 (Rspo3) 在信号转导中的作用单核细胞到肺巨噬细胞。在目标 2 中，我们将确定代谢重编程和表观遗传的作用巨噬细胞在介导表型转变中的修饰，从而减少肺损伤的程度并推动其解决。