Imaging, Histology and Functional Diagnostics Core

影像、组织学和功能诊断核心

基本信息

批准号：
10681428
负责人：
Janet Ruthe Sparrow
金额：
$ 32.26万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10681428
关键词：
3D Print Achievement Adult Angiography Animal Disease Models Animal Experimentation Animal Model Animals Area Brain Buffers Clinical Clinical Research Collaborations Color Computer software Core Facility Core Grant Cornea Dedications Development Diagnostic Diagnostic tests Disease Doctor of Philosophy Electroretinography Embryo Ensure Equipment Equipment and Supplies Eye Faculty Film Fixatives Floor Funding Fundus Glaucoma Goals Histologic Histological Techniques Histology Human Resources Human Subject Research Image Imaging Device Individual Investigation Knowledge Laboratory Technicians Lasers Learning Letters Light Maintenance Microscopic Microtome - medical device Mission Modality Molds Monkeys Mus Neonatal Nursing Faculty Ophthalmology Ophthalmoscopes Optical Coherence Tomography Oryctolagus cuniculus Paraffin Pathology Patients Pattern Photoreceptors Postdoctoral Fellow Preparation Procedures Productivity Protocols documentation Qualifying Rattus Research Research Personnel Resolution Resources Retina Retinal Ganglion Cells Retirement Rodent Role Scanning Schedule Scientist Services Sparrows Specialist Stains Structure Structure of retinal pigment epithelium Students Study Section System Techniques Testing Thick Thinness Tissues Training Translational Research United States National Institutes of Health Universities Vision Vision research Water Work animal facility brain pathway clinical application cost cryostat equipment acquisition experience fundus imaging graduate student in vivo in vivo imaging instrument instrumentation lens multimodality pathogen postnatal programs recruit repaired research faculty skills student training time use tissue preparation tissue processing tool translational study transmission process

项目摘要

Summary/Abstract The broad goals of this Core are to provide facilities for vision scientists at Columbia University. Resources are provided for imaging, histology and electroretinographic recording. The module is administered so as to ensure equipment access, technical support and personnel training. The availability of shared state-of-the-art equipment and facilities operated by trained and highly qualified technicians with oversight by vision faculty substantially increases the quality and quantity of achievements earned by research programs. Productive functioning of these core facilities will i) facilitate collaborative efforts; ii) enable the tackling of gaps in our knowledge that require multiple areas of expertise; enhance the productivity of ongoing research making research funds more profitable; and enable the development of new research strategies. The clustering of clinically applicable instruments with in the Core enhances interactions between research and clinical faculty and encourages translational research. The availability of the resources of the Core is also an advantage when recruiting new faculty. The core provides training for students and postdoctoral associates in in vivo eye imaging using modalities of short-wavelength fundus autofluorescence, near-infrared fundus autofluorescence and optical coherence tomography (OCT). Training in tissue processing and sectioning for histological analysis is also made available as is training in electroretinographic (ERG) recording of visual function. The Core supports a clinical grade animal-dedicated confocal laser scanning ophthalmoscope (Heidelberg) equipped for short-wavelength fundus autofluorescence (AF), near-infrared fundus autofluorescence, angiography and spectral domain optical coherence tomography (SD-OCT). Two new high resolution Bioptigen SD-OCT (Envisu R2210 and R4310) enable non-contact in vivo SD-OCT imaging of the eye in animal models. This capability will advance investigations of cornea, tear film, lens, glaucoma and retinal structure in both the diseased and healthy eye. The instruments enable the study of animal models of disease in parallel with human subjects research. The core maintains 3 instruments for ERG testing. These machines are used to test the function of retinal pigment epithelium (bleach protocol, c-wave), photoreceptors (scotopic and photopic serial intensities), and retinal ganglion cells in rodents. Skilled personnel under the direction of Dr. Sparrow manage day to day activities including training in the use of the instruments. Reserving and tracking of usage time and scheduling of maintenance together with other day- to-day activities are managed by iLAB software.

摘要/摘要该核心的广泛目标是为哥伦比亚大学的视觉科学家提供设施。资源是提供成像，组织学和电子图记录。管理模块以确保设备访问，技术支持和人员培训。共享最新设备的可用性以及由经过培训且高素质的技术人员经营的设施，并受到视觉教师的监督提高了研究计划所获得的成就的质量和数量。生产力的功能这些核心设施将i）促进合作努力； ii）在我们的知识中，使差距能够解决差距需要多个专业领域；提高正在进行的研究的生产力，使研究基金更多有利可图;并能够制定新的研究策略。临床适用的聚类具有核心的乐器增强了研究与临床教师之间的相互作用，并鼓励翻译研究。招募新的核心资源的可用性也是一个优势学院。该核心为学生和博士后同伴提供了体内眼成像的培训短波长的眼底自动荧光，近红外底面自动荧光和光学连贯性断层扫描（OCT）。还提供了组织加工和分区的组织学分析的培训视觉功能的电网图（ERG）记录的训练也是如此。该核心支持临床级动物范围的共聚焦激光扫描眼镜镜（海德尔伯格）配备了短波长的眼底自动荧光（AF），近红外的眼睛自动荧光，血管造影和光谱域光学相干断层扫描（SD-OCT）。两个新的高分辨率Bioptigen SD-OCT（Envisu R2210和R4310）在动物模型中对眼睛的体内SD-OCT成像启用了非接触。这种能力将推动对两者的角膜，泪膜，镜头，青光眼和视网膜结构的研究患病和健康的眼睛。这些仪器能够与人类并行研究疾病模型受试者研究。核心维护3种用于ERG测试的工具。这些机器用于测试视网膜的功能色素上皮（漂白剂方案，C波），感光器（SCOTOPIC和PHOTOPIC系列强度）和啮齿动物中的视网膜神经节细胞。在Sparrow博士的指导下，熟练的人员管理日常活动，包括使用乐器。保留和跟踪使用时间和安排维护以及其他一天 - 今天的活动由ILAB软件管理。