Acquisition of a Fluidigm C1 Single-Cell Auto Prep System

收购 Fluidigm C1 单细胞自动制备系统

• 批准号: 8825724
• 负责人: STEVEN R. GILL
• 金额: $ 21.33万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8825724
• 关键词: Area; Bioinformatics; Biology; Cancer Biology; Cancer Center; Cells; Clonal Evolution; Clonality; Consultations; Data Analyses; Dentistry; Faculty; Funding; Future; Gene Expression; Genome; Genomics; Goals; Grant; High-Throughput Nucleotide Sequencing; Laboratories; Libraries; Medicine; Methods; Minor; Mission; Molecular Profiling; Monitor; Population; Preparation; Research; Research Design; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Scientist; Stem Cell Research; Stem cells; System; Technology; United States National Institutes of Health; Universities; base; cancer type; cost effective; experience; genome sequencing; genome-wide analysis; insight; instrument; instrumentation; interest; medical schools; member; response; transcriptome sequencing

 DESCRIPTION (provided by applicant): Single-cell genome and transcriptome sequencing methods have transformed many areas of biology and medicine. These methods enable scientists to identify biologically relevant differences between single cells in a population at higher resolution than previously possible. The goal of this proposal is to acquire funds for a Fluidigm C1 Single-Cell Auto Prep system that will enable the University of Rochester Genomics Research Center (GRC) to provide single-cell genome resources to our faculty and investigators. The GRC,, located within the James P. Wilmot Cancer Center (JPWCC) at the University of Rochester School of Medicine and Dentistry (SMD), is a shared resource laboratory whose mission is to provide high-throughput genome-level analysis to all University faculty and investigators. High-throughput sequencing resources in the GRC include an Illumina HiSeq2500 that was acquired through a 2013 NIH Shared Instrumentation Grant and an Illumina MiSeq that was purchased with internal University funds. The Fluidigm C1 Single-Cell Auto Prep system represents a significant advance in genome-wide analysis at single-cell resolution. Using the C1 Auto Prep in conjunction with existing GRC high-throughput sequencing resources, investigators on the University Main Campus (UR) and SMD will be able to explore several areas of interest, including: 1) determine gene expression profiles of phenotypically defined subpopulations of cells to give insight into heterogenetic responses, 2) investigate clonality and clonal evolution o various cancer types and 3) identify and monitor expression profiles unique to stem cells and their progeny. The Fluidigm instrument will primarily be used by a group of 5 Major and 5 Minor Users who are members of an existing strong Illumina user base with a diversity of NIH funded projects. Support provided by the GRC for high-throughput sequencing includes initial consultation on study design and rationale, sample preparation, library construction, sequencing and Bioinformatic data analysis. Computational support for the GRC is integrated with the Center for Integrated Research Computing (CIRC). These combined resources and support will allow experienced investigators as well as investigators unfamiliar with single-cell genomics to take immediate advantage of the Fluidigm system. Addition of Fluidigm technology as a cost effective shared resource will enable UR/SMD investigators to be at the forefront of cancer biology and stem cell research, provide a substantial benefit to multiple NIH funded projects and stimulate additional projects among new investigators, enabling them develop future NIH funded projects and compete at the highest scientific level.

 描述（由申请人提供）： 单细胞基因组和转录组测序方法已经改变了生物学和医学的许多领域，这些方法使科学家能够以比以前更高的分辨率识别群体中单细胞之间的生物学相关差异。 Fluidigm C1 单细胞自动制备系统将使罗切斯特大学基因组学研究中心 (GRC) 能够为我们的教师和研究人员提供单细胞基因组资源，该中心位于 James P. Wilmot 癌症中心 (JPWCC)。在罗彻斯特大学医学和牙科学院 (SMD) 是一个共享资源实验室，其使命是为所有大学教职员工和研究人员提供高通量基因组水平分析。 GRC 中的高通量测序资源包括 Illumina HiSeq2500， Fluidigm C1 单细胞自动制备系统是通过 2013 年 NIH 共享仪器拨款和大学内部资金购买的 Illumina MiSeq 获得的，代表了一项重大进步。利用 C1 Auto Prep 与现有的 GRC 高通量测序资源进行单细胞分辨率的全基因组分析，大学主校区 (UR) 和 SMD 的研究人员将能够探索多个感兴趣的领域，包括： 1) 确定表型定义的细胞亚群的基因表达谱，以深入了解异基因反应，2) 研究各种癌症类型的克隆性和克隆进化，3) 识别和监测干细胞及其后代特有的表达谱。 Fluidigm 仪器将主要由 5 个主要用户和 5 个次要用户组成，这些用户是 Illumina 现有强大用户群的成员，拥有 NIH 资助的各种项目，GRC 为高通量测序提供支持，包括研究设计的初步咨询。 GRC 的基本原理、样品制备、文库构建、测序和生物信息学数据分析与综合研究计算中心 (CIRC) 相结合，这些综合资源和支持将为经验丰富的研究人员和不熟悉的研究人员提供支持。单细胞基因组学立即利用 Fluidigm 系统 作为一种具有成本效益的共享资源，Fluidigm 技术的加入将使 UR/SMD 研究人员能够处于癌症生物学和干细胞研究的前沿，为多个 NIH 资助的机构带来巨大利益。项目并刺激新研究人员开展更多项目，使他们能够开发未来 NIH 资助的项目并在最高科学水平上竞争。