Imaging and Surgery Core

影像和手术核心

• 批准号: 10674672
• 负责人: Gwendalyn J Randolph
• 金额: $ 45.09万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10674672
• 关键词: Antibodies; Behavior; Biology; Blood Vessels; Blood flow; Brain region; Cells; Collaborations; Color; Coupled; Data; Data Analyses; Data Set; Diameter; Erythrocytes; Grant; High Density Lipoproteins; Image; Image Analysis; Image-Guided Surgery; Immune; Individual; Laboratories; Lipoproteins; Lymphatic; Macrophage; Meningeal; Meninges; Methods; Modality; Molecular; Natural Immunity; Operative Surgical Procedures; Oxygen; Pathogenesis; Pathology; Penetration; Procedures; Reagent; Refractory; Research; Research Personnel; Resolution; Role; Sampling; Science; Services; Speed; Stains; Standardization; Surgeon; Technology; Thinness; Three-Dimensional Imaging; Tissue imaging; Tissues; Tracer; Transplantation; combinatorial; cranium; experience; improved; indexing; innovation; intravital imaging; light microscopy; lymphatic vasculature; mouse model; neuroimmunology; novel; oxygen transport; photoacoustic imaging; programs; protocol development; skills; tissue fixing; tool; trafficking; two photon microscopy; two-photon

ABSTRACT – Core B The imaging and surgical core will provide state-of-the-art imaging services to the program. Dr. Randolph’s experience in organizing collaborative teams and her laboratory's background in macrophage biology and lymphatic/vascular trafficking of immune cells and lipoproteins will facilitate applications in imaging that will be tailored to the science of each project. The core will incorporate major contributions from highly expert co- investigators. Dr. Bernd Zinselmeyer is an expert in multiple modalities of light microscopy, including its application in studies of the CNS. He will contribute several lines of expertise in the grant, from confocal and 2- photon microscopy to high-speed epifluorescence imaging. Dr. Song Hu is an innovator in photoacoustic imaging with demonstrated expertise in using this technology to study the vasculature of the CNS. Dr. Igor Smirnov is an expert microsurgeon who will provide and oversee highly skilled surgical manipulations for the projects, including procedures like skull transplant and skull thinning. These co-investigators bring more than their state-of-the-art expertise; they also all bring demonstrated collaborations with one or more project leaders. By working together, this core team of investigators and affiliated staff will offer to all projects the following range of expert surgical procedures and imaging applications. The aims of the imaging and surgical core are to (1) carry out deep-tissue imaging of meninges and outer regions of the brain, (2) apply combinatorial methods (2-photon, high-speed widefield, and photoacoustic imaging) for intravital imaging to investigate macrophage behavior and blood flow dynamics in the meninges, and (3) employ established and novel tools to quantify molecular outflow from the meningeal space.

摘要 - 核心B 成像和手术核心将为程序提供最新的成像服务。兰道夫博士 在组织合作团队及其实验室在巨噬细胞生物学方面的背景方面的经验和 免疫细胞和脂蛋白的淋巴/血管运输将促进成像中的应用 根据每个项目的科学量身定制。核心将结合高度专家共同的主要贡献 调查人员。 Bernd Zinselmeyer博士是多种光学显微镜的专家，包括其 在中枢神经系统研究中的应用。他将在赠款中贡献几条专业知识，共聚焦和2-- 光子显微镜至高速落荧光成像。 Song Hu博士是光声成像的创新者 在使用这项技术研究中枢神经系统的脉管系统方面具有专业知识。 Igor Smirnov博士是 专业的微型外科医生将为项目提供并监督高技能的手术操纵 诸如头骨移植和颅骨稀疏之类的程序。这些共同投资者带来的不仅仅是最先进的 专业知识;他们还与一个或多个项目负责人进行了演示的合作。通过一起工作， 这个调查人员和会员人员组成的核心团队将为所有项目提供以下专家手术范围 程序和成像应用。成像和手术核心的目的是（1）进行深度组织 脑膜和大脑外部区域的成像，（2）采用组合方法（2-Photon，高速 广阔的菲尔德和光声成像）用于浸润成像以研究巨噬细胞行为和血流 脑膜中的动态和（3）员工建立了新的工具，可以量化从 脑膜空间。