Development and characterization of optical imaging probes

光学成像探头的开发和表征

基本信息

批准号：
10699713
负责人：
Keir Neuman
金额：
$ 17.42万
依托单位：
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10699713
关键词：
Address Antibodies Binding Blinking Bromides Carbon nanoparticle Carboxylic Acids Cesium Characteristics Chemical Structure Chemistry Detection Development Diamond Drug Delivery Systems Dyes Fingerprint Fluorescence Fluorescent Probes Goals In Vitro Individual Label Lead Magnetism Measurement Measures Methods Microscope Multimodal Imaging Nitrogen Noise Nucleosome Core Particle Optics Penetration Property Protocols documentation Publishing Scheme Side Signal Transduction South Korea Surface Surface Properties Techniques Testing Tissues United States National Institutes of Health Universities Woman Work biomaterial compatibility density fluorescence imaging fluorophore functional group imaging approach imaging probe imaging study improved in vivo in vivo evaluation in vivo imaging individual response interest molecular imaging multimodality nano nanocrystal nanodiamond nanoparticle nanoparticle delivery novel novel strategies optical imaging particle perovskite single molecule

项目摘要

Currently, there is one overarching project focused on functionalizing and characterizing fluorescent nanodiamonds: We are working on functionalizing and characterizing nitrogen vacancy center fluorescent nanodiamonds (FNDs) for use as multi-modal imaging probes. These are attractive fluorescence particles for in vivo and in vitro tracking and imaging studies as they are bright, non-blinking fluorophores that are excited in the green (560 nm) and emit in the far red spectrum (680-800 nm), which has superior tissue penetration and signal-to-noise characteristics compared with shorter wavelengths. Moreover, diamond is inert and the fluorescence arises from the nitrogen vacancy so the core particle contains no organic dyes or other potentially toxic material that would be problematic for in vivo applications. Remarkably, the FNDs can be as small as 10 nm, which is also advantageous for biocompatibility and clearing. We have established protocols to functionalize FNDs for biomedical applications. We recently published work describing methods to enhance the density of carboxylic acid groups on the surface of FNDs, and demonstrated that this approach enables the robust functionalization of FNDS with more than 10 different biologically important functional groups. This work provides a means of overcoming the inherent variability and inhomogeneity in the surface properties of FNDs and other carbon nanoparticles. Following up on our work developing FNDs as biocompatible multimodal probes for the development and detection of latent fingerprints, we worked with our collaborator, Junsang Cho, in the Department of Chemistry, Duksung Women's University, South Korea, to develop highly stanble cesium lead bromide perovskite nanocrystals as fluorescent probes for the detection of latent fingerprints. Work is continuing to expand the functionalization of the FNDs to produce multi-modal particles that can serves as both targeted fluorescent nanoparticles and drug delivery agents employing a novel mesoporous polydopamine encapsulation approach. Using a recently developed micro-mirror TIRF microscope combined with a magnetic tweezers, we developed a method to simultaneously determine the size and brightness of individual FNDs in a massively parallel measurement. This is an important measurement for the characterization of FNDs that has previously been exceedingly low throughput. This new approach allow affords us the opportunity to simultaneously measure the magnetic response of individual FNDs as a function of their size and brightness.

当前，有一个总体项目着重于功能化和表征荧光纳米木吗？我们正在努力化和表征氮空位中心荧光纳米座（FND），以用作多模式成像探针。这些是具有吸引人的荧光颗粒，用于体内和体外跟踪和成像研究，因为它们是明亮的，非闪烁的荧光团，在绿色（560 nm）中激发并在远红色谱（680-800 nm）中发射，与更短的组织穿透性和信号效率相比，它具有较高的组织穿透性和信号性特征。此外，钻石是惰性的，荧光是由氮的空缺产生的，因此核心颗粒不含有机染料或其他潜在有毒物质，这对于体内应用而言是有问题的。值得注意的是，FND可以小至10 nm，这对于生物相容性和清除也是有利的。我们已经建立了为生物医学应用功能功能化FND的协议。我们最近发表了描述方法来增强FND表面上羧酸基团密度的方法，并证明该方法能够具有10多种不同生物学上重要的功能组的FND的鲁棒功能化。这项工作提供了克服FND和其他碳纳米颗粒表面特性中固有的可变性和不均匀性的手段。 Following up on our work developing FNDs as biocompatible multimodal probes for the development and detection of latent fingerprints, we worked with our collaborator, Junsang Cho, in the Department of Chemistry, Duksung Women's University, South Korea, to develop highly stanble cesium lead bromide perovskite nanocrystals as fluorescent probes for the detection of latent fingerprints. 工作正在继续扩大FND的功能化，以产生可以用作靶向荧光纳米颗粒和使用新型介孔多巴胺封装方法的多模式颗粒。使用最近开发的微型晶状体TIRF显微镜与磁镊子结合使用，我们开发了一种方法来同时确定在大量平行测量中单个FND的大小和亮度。这是对以前吞吐量非常低的FND表征的重要测量。这种新方法使我们有机会同时测量单个FND的磁反应，这是其大小和亮度的函数。