Coordination Core

协调核心

基本信息

批准号：
10700253
负责人：
David W. Rowe
金额：
$ 53.5万
依托单位：
UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-21 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10700253
关键词：
3-Dimensional Address Adoption Advocate Affect Awareness Back Bar Codes Biology Biopsy Cartilage Cell physiology Cells Cellular Structures Collaborations Communities Complex Confocal Microscopy Data Data Analyses Dental Discipline Disease Educational workshop Ensure Environment Ethnic Origin Faculty Gene Expression Profile Heterogeneity Histologic Human Human BioMolecular Atlas Program Image Individual Institutional Review Boards Intuition Joints Laboratories Lead Leadership Letters Logistics Management Information Systems Maps Metadata Minerals Molecular Output Participant Pathologic Processes Pathway interactions Process Protocols documentation Publications Quality Control RNA Regulation Research Resolution Resource Development Resources Role Sampling Scanning Shapes Skeleton Structure System Technology Testing Tissue Embedding Tissues Tooth structure Visual arm base career development cell behavior cell type data management data repository data sharing expectation experience high resolution imaging histological image human disease information display interest meetings member mineralization multidisciplinary multimodality outreach preference programs recruit scientific organization sex skeletal skeletal tissue skills substantia spongiosa tool

项目摘要

ABSTRACT: COORDINATION CORE The coordination core has an administrative and scientific function. It will initiate the administrative process of procuring the human skeletal tissues that will be used by the mineralized tissue project. Our LIMS will be modified to capture all the workflow activities from the acquisition to handoff to the mineralized tissue project group. This includes recording the orientation of the sample relative to the tissue of origin, obtaining a µCT scan of the mineral distribution, embedding the tissue for serial sectioning that incorporates four registration points that define a grid structure for cell localization. After widefield histological images are recorded, the same sections are transferred to the mineralization core for the seqFISH studies that identify cell type and individual cell RNA transcriptone profile. Using the same grid system, a 3D cell map will be generated by the data analysis core which in turn will interact with the coordination core to align the histological and 3D cell map. Together the two cores will build a visual representation of the cells within a tissue block that interchangeably relate a specific cell with its transcriptional profile and the active molecular pathways. In an iterative process that will be driven by the coordination core, quality control standards for identification of cell types as well as subdivisions within a cell type will be developed that will serve as the basis for imputing the activity of interacting cells or comparing differences in regulation of cell behavior that underlie dimorphic differences based on sex or ethnicity. As we become proficient in the technologies and data management process of cell mapping, we will shape our data presentation format to that used by other members of the HIVE. By direct interaction with other scientific groups in HuBMAP, we hope to expand the multimodal interrogation of our skeletal tissues and contribute to the technological capabilities of other HuBMAP members. The coordination core will also reach out to the skeletal biology community to make them aware of the resources of the HIVE and provide workshops to help other major skeletal biology groups implement this technology in their research environment. The technologies and concepts of HuBMAP will be transformational for understanding normal and pathological processes in human disease. We want to adapt this experimental platform to tissues of the mineralized skeleton and advocate for its adoption throughout the skeletal research community.

摘要：协调核心协调核心具有行政和科学职能，它将启动行政管理。我们的矿化组织项目将使用的人体骨骼组织的采购过程。 LIMS 将进行修改，以捕获从采集到移交到交付的所有工作流程活动。这包括记录样本相对于矿化组织的方向。原始组织，获得矿物分布的 µCT 扫描，包埋组织以进行连续处理包含四个注册点的切片，这些注册点定义了用于细胞定位的网格结构。记录宽场组织学图像，将相同的切片转移到矿化核心用于识别细胞类型和单个细胞 RNA 转录谱的 seqFISH 研究。在相同的网格系统中，数据分析核心将生成 3D 单元图，而该图又将进行交互与协调核心对齐组织学和 3D 细胞图谱，这两个核心将共同构建一个。组织块内细胞的视觉表示，可将特定细胞与其可互换地联系起来在由其驱动的迭代过程中。协调核心、细胞类型和细分鉴定的质量控制标准将开发细胞类型内的模型，作为估算相互作用活性的基础细胞或比较基于二态性差异的细胞行为调节差异随着我们精通细胞技术和数据管理流程。映射，我们将根据 HIVE 其他成员使用的格式调整我们的数据呈现格式。与 HuBMAP 中其他科学小组的直接互动，我们希望扩大多模式询问我们的骨骼组织并为其他 HuBMAP 的技术能力做出贡献协调核心还将联系骨骼生物学界来制作它们。了解 HIVE 的资源并提供研讨会来帮助其他主要骨骼生物学团体在他们的研究环境中实施该技术 HuBMAP 的技术和概念。对于理解人类疾病的正常和病理过程将具有革命性的意义。希望使这个实验平台适应矿化骨骼的组织并倡导其整个骨骼研究界的采用。