Pathogenic Mechanism of Intrathecally Synthesized Immunoglobulins in a Mouse Model of Progressive Multiple Sclerosis

鞘内合成免疫球蛋白在进行性多发性硬化症小鼠模型中的致病机制

• 批准号: 10673659
• 负责人: Michael R Linzey
• 金额: $ 0.73万
• 依托单位: DARTMOUTH-HITCHCOCK CLINIC
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-22 至 2023-09-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10673659
• 关键词: Affect; Agreement; Anaphylatoxins; Antibodies; Antigen-Antibody Complex; Antigens; Area; Automobile Driving; Axon; Binding; Biological Markers; Cells; Central Nervous System; Central Nervous System Diseases; Cerebrospinal Fluid; Characteristics; Chronic; Chronic Phase of Disease; Classical Complement Pathway; Clinical; Cognitive; Complement; Complement 1q; Complement-Dependent Cytotoxicity; Complex; Demyelinating Diseases; Demyelinations; Deposition; Development; Disease; Disease Outcome; Disease Progression; Engineering; Evaluation; Flow Cytometry; Gene Expression; Goals; Histologic; Histology; Imaging Techniques; Immune; Immunofluorescence Immunologic; Immunoglobulins; Immunologics; Inflammation; Inflammation Mediators; Inflammatory; Intraventricular; Lesion; Light; Lytic; Magnetic Resonance Imaging; Manuscripts; Measurement; Mentors; Modeling; Motor; Multiple Sclerosis; Mus; Nerve Degeneration; Nervous System Trauma; Neurodegenerative Disorders; Neurologic Symptoms; Opsonin; Outcome; Pathogenicity; Pathologic; Pathology; Phenotype; Play; Preparation; Proteins; Relapse; Role; Severity of illness; TMEV; Techniques; Testing; Therapeutic; Tissues; Work; antibody and antigen binding; antibody-dependent cell cytotoxicity; axon injury; blood-brain barrier crossing; chemokine; chronic inflammatory disease; cognitive function; complement pathway; complement system; cytokine; cytotoxic; cytotoxicity; diagnostic criteria; disability; drug discovery; follow-up; glial activation; gray matter; improved; in vivo; insight; molecular diagnostics; mouse model; multiple sclerosis patient; nanobodies; neurodegenerative phenotype; neurofilament; neuroinflammation; neuron loss; neuroprotection; novel; prevent; recruit; spatial relationship; success; white matter

Project Summary/Abstract Relapsing and progressive multiple sclerosis (MS) are characterized by an intrathecal synthesis of immunoglobulins (IIgS). This occurs throughout the disease and is the only widely agreed upon molecular diagnostic criteria for MS. A growing body of evidence also suggests that IIgS correlates with MS disease progression, although the pathological role of these antibodies remains to be determined. Cytotoxic activities in the central nervous system (CNS) are critical determinants of MS disease progression. Interestingly, antibodies can activate two distinct cytotoxic mechanisms: antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Therefore, I initially hypothesized that, in MS, IIgS triggers ADCC and CDC within the CNS, thereby accruing CNS damage and worsening disease progression. Thus, over the past few years, the primary goal of my work has been to define the cytotoxic activity of these Igs by using Theiler’s murine encephalomyelitis virus-induced demyelinating disease (TMEV-IDD) as a model of progressive MS. Particularly, I have been focusing on CDC and the classical complement pathway. The first component of the classical complement pathway (C1q) recognizes and binds to Igs complexed to antigen and initiates the complement cascade, ultimately triggering CDC. Since the results of my studies suggest higher levels of C1q in the CNS indicate more severe clinical and pathological disease, I developed a refined working hypothesis. Thus, I now hypothesize that therapeutic inhibition of C1q in the CNS of TMEV-IDD mice will prevent disease progression by reducing neuroaxonal damage and neuroinflammation. I will test this hypothesis by accomplishing the following three aims: Aim 1 will demonstrate that anti-C1q treatment improves clinical outcomes. To accomplish this aim, TMEV- IDD mice will be treated with either an intraventricularly injected murine anti-C1q antibody (M1) or a systemically administered anti-C1q camelid VHH antibody (aka nanobody). Motor and cognitive outcomes will be evaluated. Aim 2 will demonstrate that anti-C1q treatment reduces neuroaxonal damage. To accomplish this aim, neuroaxonal damage in treated vs. untreated mice will be assessed throughout the disease by 1) measurement of cerebrospinal fluid (CSF) biomarkers of neuroaxonal damage, 2) histology, and 3) quantitative magnetic resonance imaging (MRI) techniques. Aim 3 will demonstrate that anti-C1q treatment reduces neuroinflammation. To accomplish this aim, neuroinflammation will be assessed in treated and untreated mice by 1) flow cytometry, 2) immunofluorescence, and 3) measurement of intrathecally produced inflammatory biomarkers. Overall, this work will provide compelling evidence for the significant role played by IIgs in PMS, thereby defining a new paradigm for its treatment.

项目摘要/摘要 复发和进行性多发性硬化症（MS）的特征是鞘内合成 免疫球蛋白（IIG）。这发生在整个疾病中，是唯一一致的分子 MS的诊断标准。越来越多的证据也表明IIG与MS疾病相关 尽管这些抗体的病理作用仍有待确定。 中枢神经系统（CNS）中的细胞毒性活性是MS疾病进展的关键决定者。 有趣的是，抗体可以激活两种不同的细胞毒性机制：抗体依赖性细胞介导 细胞毒性（ADCC）和补体依赖性细胞毒性（CDC）。因此，我最初假设 MS，IIGS在中枢神经系统内触发ADCC和CDC，从而造成CNS损害和令人担忧的疾病 进展。在过去的几年中，我工作的主要目标是定义细胞毒性活性 通过使用Theiler的鼠脑脊髓炎病毒诱导的脱髓鞘疾病（TMEV-IDD）作为一种Ig 进行性MS的模型。特别是，我一直专注于CDC和经典补体途径。 经典补体途径（C1Q）的第一个组成部分识别并结合了IGS 抗原并启动完成级联，最终触发CDC。由于我的研究结果表明 中枢神经系统中较高水平的C1Q表明更严重的临床和病理疾病，我开发了一种精制 工作假设。我现在假设对TMEV-IDD小鼠中枢神经系统中C1Q的治疗性抑制作用 将通过减少神经司长损伤和神经炎症来预防疾病进展。我会测试这个 通过完成以下三个目标来假设： AIM 1将证明抗C1Q治疗可改善临床结果。为了实现这一目标，TMEV- IDD小鼠将用静脉注射的鼠抗C1Q抗体（M1）或全身治疗 施用的抗C1Q Camelid VHH抗体（又称纳米病）。将评估运动和认知结果。 AIM 2将证明抗C1Q治疗可减少神经司长损伤。为了实现这一目标， 在治疗中与未处理的小鼠中的神经司长损伤将在整个疾病中评估1）测量 脑脊液（CSF）神经司长损伤的生物标志物，2）组织学和3）定量磁性 共振成像（MRI）技术。 AIM 3将证明抗C1Q治疗减少神经炎症。为了实现这一目标， 神经炎症将在处理和未处理的小鼠中评估1）流式细胞仪，2）免疫荧光， 3）测量鞘内产生的炎症生物标志物。 总体而言，这项工作将为IIG在PMS中扮演的重要作用提供令人信服的证据 定义新的范式以进行治疗。