Maternal obesity, AMPK and Developmental Programming
孕产妇肥胖、AMPK 和发育规划
基本信息
- 批准号:10672327
- 负责人:
- 金额:$ 37.71万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-09-27 至 2027-07-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Maternal obesity, AMPK and Developmental Programming
Over 30% of pregnant American women are obese and an additional 30% are over-weight, conditions which
negatively affect fetal development with long-term consequences for offspring health, including pre-disposition
to obesity and type 2 diabetes (T2D). The underlying mechanisms remain poorly defined. Skeletal muscle (SM)
accounts for >30% body weight and is a key tissue for the oxidation of fatty acids and glucose, as is brown
adipose tissue (BAT). Under the support of our previous award, we demonstrated that maternal obesity (MO)
elicits early onset of fibrotic and fatty infiltration (FFI) in offspring SM and BAT, which impairs their functions
and programs metabolic disorders in offspring. We found that AMP-activated protein kinase (AMPK), a known
target for metformin, is robustly inhibited due to MO, correlates with FFI and worsened offspring SM/BAT
functions. In the early embryo, both myogenic and fibrogenic cells are derived from progenitor cells (PCs) in
the dermomyotome. While the embryonic myogenic cells are the sources of both myogenic and brown
adipogenic cells, embryonic fibrogenic cells are sources of fibrogenic and white adipogenic cells in offspring
SM/BAT. Because of this, our previous studies point to embryonic origins for developmental abnormalities of
offspring SM/BAT due to MO, but this is yet to be examined. Using single cell RNA sequencing (scRNA-seq),
we found that embryonic myogenesis is attenuated in E9.5 MO embryos. We hypothesize that MO suppresses
AMPK, which inhibits myogenic commitment and drives uncommitted PCs to fibrogenesis during embryonic
development, programming FFI in offspring SM/BAT. Accordingly, we have three specific aims: 1) Study AMPK
inhibition due to MO in impairing embryonic myogenic commitment; 2) Evaluate AMPK in linking MO to
enhanced embryonic fibrogenesis; and 3) Analyze AMPK as a target for improving embryonic SM/BAT
development of MO and the resulting offspring SM/BAT functions. We will use single cell “omics” for analyzing
embryonic tissues, and embryoid body culture for mechanistic exploration. Knowledge obtained will identify
molecular targets for therapeutics to improve embryonic SM/BAT development and subsequent metabolic
health of MO offspring, helping the increasing populations of obese mothers to deliver healthy children.
孕产妇肥胖,AMPK和发育节目
超过30%的孕妇妇女肥胖,另外30%的疾病是
负面影响胎儿的发育,对后代健康的长期后果,包括前置前的影响
肥胖和2型糖尿病(T2D)。基本机制的定义仍然很差。骨骼肌(SM)
占体重> 30%的体重,是氧化脂肪酸和葡萄糖的关键组织,棕色也是
脂肪组织(蝙蝠)。在我们以前的奖项的支持下,我们证明了物物肥胖症(MO)
在后代SM和BAT中引起纤维化和脂肪浸润(FFI)的早期发作,这会损害其功能
和后代的代谢疾病。我们发现AMP激活的蛋白激酶(AMPK)是已知的
二甲双胍的靶标,由于MO而受到牢固抑制,与FFI相关并恶化后代SM/BAT
功能。在早期的胚胎中,肌原性和纤维基因均源自祖细胞(PC)
皮肤病组。胚胎肌原性细胞是肌原性和棕色的来源
掺杂细胞,胚胎纤维生成细胞是后代中纤维生成和白色掺杂细胞的来源
SM/蝙蝠。因此,我们以前的研究指出了胚胎起源于发育异常
由于mo而导致的后代SM/蝙蝠,但这尚待检查。使用单细胞RNA测序(SCRNA-SEQ),
我们发现胚胎肌发生在E9.5 mo胚胎中减弱。我们假设MO抑制
AMPK,它抑制肌源性承诺并在胚胎期间驱动未承诺的PC进行纤维化
开发,后代SM/BAT的编程FFI。根据,我们有三个具体的目标:1)研究AMPK
由于MO引起的抑制作用会损害胚胎肌源性承诺; 2)评估将MO链接到与的AMPK
增强的胚胎纤维发生; 3)分析AMPK作为改善胚胎SM/BAT的目标
MO的开发以及由此产生的后代SM/BAT功能。我们将使用单个单元格进行分析
胚胎组织和用于机械探索的胚胎体培养。获得的知识将确定
用于改善胚胎SM/BAT发育和随后代谢的治疗的分子靶标
莫后代的健康,帮助肥胖母亲越来越多的人群分娩健康的孩子。
项目成果
期刊论文数量(59)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
AMPK/α-Ketoglutarate Axis Dynamically Mediates DNA Demethylation in the Prdm16 Promoter and Brown Adipogenesis.
- DOI:10.1016/j.cmet.2016.08.010
- 发表时间:2016-10-11
- 期刊:
- 影响因子:29
- 作者:Yang Q;Liang X;Sun X;Zhang L;Fu X;Rogers CJ;Berim A;Zhang S;Wang S;Wang B;Foretz M;Viollet B;Gang DR;Rodgers BD;Zhu MJ;Du M
- 通讯作者:Du M
Myostatin Attenuation In Vivo Reduces Adiposity, but Activates Adipogenesis.
- DOI:10.1210/en.2015-1546
- 发表时间:2016
- 期刊:
- 影响因子:4.8
- 作者:Naisi Li;Qiyuan Yang;R. G. Walker;T. Thompson;M. Du;B. D. Rodgers
- 通讯作者:Naisi Li;Qiyuan Yang;R. G. Walker;T. Thompson;M. Du;B. D. Rodgers
Embryonic exposure to hyper glucocorticoids suppresses brown fat development and thermogenesis via REDD1.
- DOI:10.1016/j.scib.2020.10.015
- 发表时间:2021-03-15
- 期刊:
- 影响因子:18.9
- 作者:Chen YT;Hu Y;Yang QY;Liu XD;Son JS;de Avila JM;Zhu MJ;Du M
- 通讯作者:Du M
Nutrigenomic regulation of adipose tissue development - role of retinoic acid: A review.
- DOI:10.1016/j.meatsci.2016.04.003
- 发表时间:2016-10
- 期刊:
- 影响因子:7.1
- 作者:Wang B;Yang Q;Harris CL;Nelson ML;Busboom JR;Zhu MJ;Du M
- 通讯作者:Du M
Meat Science and Muscle Biology Symposium: extracellular matrix in skeletal muscle development and meat quality.
- DOI:10.2527/jas.2011-4937
- 发表时间:2012-03
- 期刊:
- 影响因子:3.3
- 作者:Du M;Carlin KM
- 通讯作者:Carlin KM
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MIN DU的其他基金
Maternal obesity, AMPK and fetal brown adipogenesis
母亲肥胖、AMPK 和胎儿棕色脂肪形成
- 批准号:99814279981427
- 财政年份:2010
- 资助金额:$ 37.71万$ 37.71万
- 项目类别:
Maternal obesity, AMPK and fetal brown adipogenesis
母亲肥胖、AMPK 和胎儿棕色脂肪形成
- 批准号:93800799380079
- 财政年份:2010
- 资助金额:$ 37.71万$ 37.71万
- 项目类别:
Maternal obesity, AMPK and fetal brown adipogenesis
母亲肥胖、AMPK 和胎儿棕色脂肪形成
- 批准号:97513509751350
- 财政年份:2010
- 资助金额:$ 37.71万$ 37.71万
- 项目类别:
Maternal Obesity affects AMP-Kinase in Muscle Cell Differentiation
母亲肥胖影响肌细胞分化中的 AMP 激酶
- 批准号:80230088023008
- 财政年份:2010
- 资助金额:$ 37.71万$ 37.71万
- 项目类别:
Maternal obesity, AMPK and fetal brown adipogenesis
母亲肥胖、AMPK 和胎儿棕色脂肪形成
- 批准号:1022009010220090
- 财政年份:2010
- 资助金额:$ 37.71万$ 37.71万
- 项目类别:
Maternal obesity, AMPK and Developmental Programming
孕产妇肥胖、AMPK 和发育规划
- 批准号:1053528710535287
- 财政年份:2010
- 资助金额:$ 37.71万$ 37.71万
- 项目类别:
Maternal Obesity affects AMP-Kinase in Muscle Cell Differentiation
母亲肥胖影响肌细胞分化中的 AMP 激酶
- 批准号:87055528705552
- 财政年份:2010
- 资助金额:$ 37.71万$ 37.71万
- 项目类别:
Maternal Obesity affects AMP-Kinase in Muscle Cell Differentiation
母亲肥胖影响肌细胞分化中的 AMP 激酶
- 批准号:83067448306744
- 财政年份:2010
- 资助金额:$ 37.71万$ 37.71万
- 项目类别:
Maternal Obesity affects AMP-Kinase in Muscle Cell Differentiation
母亲肥胖影响肌细胞分化中的 AMP 激酶
- 批准号:85107008510700
- 财政年份:2010
- 资助金额:$ 37.71万$ 37.71万
- 项目类别:
Maternal Obesity affects AMP-Kinase in Muscle Cell Differentiation
母亲肥胖影响肌细胞分化中的 AMP 激酶
- 批准号:81504088150408
- 财政年份:2010
- 资助金额:$ 37.71万$ 37.71万
- 项目类别:
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