Resource and Service Center

资源与服务中心

• 批准号: 10670783
• 负责人: John Richard Seavitt
• 金额: $ 42.8万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10670783
• 关键词: Address; Affect; Alleles; Animal Experiments; Animal Model; Animal Testing; Applications Grants; Archives; Area; Bioinformatics; Clinic; Communication; Communities; Community Outreach; Data Analyses; Disease; Disease model; Drosophila genus; Ensure; Environment; Fee-for-Service Plans; Genetic Engineering; Genetic Identity; Genetic Variation; Goals; Health Services Accessibility; Heart; Infrastructure; Knockout Mice; Leadership; Link; Macaca mulatta; Maintenance; Methods; Modeling; Mus; Patient risk; Patients; Phenotype; Preservation Technique; Procedures; Process; Production; Publishing; Reproducibility; Research; Research Design; Research Personnel; Resources; Rice; Risk Assessment; Sample Size; Services; Site; Standardization; Statistical Methods; Structure; Technology; Text; Translations; animal breeding; animal resource; cost; fly; gene discovery; genome editing; genome integrity; human disease; human genome sequencing; instrumentation; meetings; model organism; network models; news; operation; precision medicine; preclinical study; preservation; programs; public repository; quality assurance; screening; social media; somatic cell gene editing; web site

ABSTRACT Poor reproducibility of published research employing model organisms and lack of transparency of study designs have become areas of concern for the scientific community. These issues can profoundly affect the interpretation of preclinical studies and must be addressed as genome editing technologies permit rapid modeling of patient- specific, disease-associated genetic variation and translation to the clinic in the forms of patient risk assessment, management, and treatment. At the heart of reproducibility issues are the model organism themselves. Stability and identity of the genetic background, genome integrity, and specifics of genetically engineered alleles often vary between studies due to use of similar but independently developed models or differences in the quality of model maintenance. Centralization and standardization of high-quality production and maintenance of animal models can begin to address issues with study reproducibility and transparency and has been highly successful for our existing animal modeling programs. The Resource and Service Section of the BCM Center for Precision Medicine Modeling will build on existing expertise and infrastructures of the Undiagnosed Diseases Network Model Organism Screening Center Drosophila Core at BCM, the BCM Knockout Mouse Production and Phenotyping site, the BCM-Rice Small Animal Testing Center for the Somatic Cell Genome Editing Program, and a large rhesus macaque sequence resource in the Human Genome Sequencing Center to (1) acquire, characterize, and maintain high-quality fly and mouse resources, (2) preserve and distribute fly and mouse resources, (3) coordinate Center fee-for-service activities for biomedical researchers, (4) communicate the availability and quality of Center resources and services, and (5) enact procedures to evaluate Section operations.

抽象的 使用模型生物和缺乏研究设计的透明度的已发表研究的可重复性差 已经成为科学界关注的领域。这些问题可以深刻影响解释 临床前研究必须通过基因组编辑技术来解决，允许对患者进行快速建模 特定的，与疾病相关的遗传变异和以患者风险评估的形式转化为诊所， 管理和治疗。可重复性问题的核心是模型生物本身。稳定 遗传背景，基因组完整性和基因工程等位基因的细节的身份经常 由于使用相似但独立开发的模型或质量差异而导致的研究之间有所不同 模型维护。高质量生产和维护动物的集中和标准化 模型可以开始解决研究可重复性和透明度的问题，并且非常成功 对于我们现有的动物建模计划。 BCM精确中心的资源和服务部分 医学建模将基于未诊断疾病网络的现有专业知识和基础设施 在BCM，BCM敲除小鼠的产生和 表型位点，BCM-RICE小动物测试中心，用于体细胞基因组编辑程序， 在人类基因组测序中心（1）获取中的恒河猴猕猴序列资源， 表征并保持高质量的苍蝇和鼠标资源，（2）保存和分发苍蝇和鼠标 资源，（3）为生物医学研究人员协调中心的服务费活动，（4）交流 中心资源和服务的可用性和质量，以及（5）制定评估部分的程序 运营。