Biochemical Characterization of the CAMKV Pseudokinase

CAMKV 假激酶的生化特征

基本信息

批准号：
10666873
负责人：
Stefan W. Vetter
金额：
$ 14.5万
依托单位：
NORTH DAKOTA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10666873
关键词：
Affinity Amino Acids Antibodies Binding Binding Proteins Binding Sites Biochemical Biological Biological Assay Brain C-terminal Calmodulin Calorimetry Catalytic Domain Central Nervous System Data Development Disease Enzymes Foundations Gene Expression Genes Homologous Gene Human Immobilization Ions Knowledge Learning Length Libraries Ligand Binding Ligands Link Mass Spectrum Analysis Measures Memory Mental disorders Methods Molecular Molecular Conformation Mutation Neurons Nucleotides Pathologic Phosphotransferases Physiological Pilot Projects Play Post-Traumatic Stress Disorders Property Proteins Proteomics Psyche structure Publications Rattus Recombinants Regulation Research Role Signal Transduction Source Tail Thermodynamics Tissues Titrations Transition Temperature United States National Institutes of Health Vesicle brain tissue cell growth regulation cognitive function drug-like compound experimental study genome wide association study human disease inhibitor innovation insight interest kinase inhibitor preservation protein complex protein protein interaction scaffold

项目摘要

CAMKV is a brain specific pseudokinase and closely related to calmodulin regulated kinase 1 (CAMK1). Gene expression studies and genome wide association studies suggest a possible role of CAMKV in mental disorders, including post-traumatic stress disorder. CAMKV has been identified by the NIH – IDG initiative as a protein in need for additional research. The proposed research will contribute to the biochemical characterization of CAMKV by investigating its mechanism of regulation on the molecular level. In specific aim 1, ligands targeting the degenerated ATP binding pocket of CAMKV will be identified. Expected results, will provide insights in the cellular regulation of CAMKV by endogenous ligands and also identify small-drug like molecules as potential CAMKV inhibitors or activators. In specific aim 2, CAMKV interacting proteins will be isolated from brain tissue. Knowledge of CAMKV targets will be critically important to understand its role in normal neuronal tissue, as well as to provide a foundation for the role of CAMKV in mental diseases.

CAMKV是一种大脑特异性假子酶，与钙调蛋白常规激酶1密切相关（CAMK1）。 CAMKV的精神障碍，包括创伤后应激障碍由NIH -IDG计划确定为需要进行其他研究的蛋白质。 CAMKV的CAMKV对CAMKV的生化表征有助于支撑研究在特定目标1中研究其法规机制。靶向CAMKV的退化ATP结合袋。将通过内源配体和IDSO在CAMKV的细胞调节中提供见解小药物像分子一样作为潜在的CAMKV抑制剂或激活剂。相互作用的蛋白质将从脑组织中分离出来。了解其在正常神经元组织中的作用至关重要，以提供 CAMKV在精神疾病中的作用的基础。