Lightsheet Microscope

光片显微镜

• 批准号: 10632804
• 负责人: George M Smith
• 金额: $ 59.98万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-15 至 2024-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632804
• 关键词: Blood Vessels; Cells; Collaborations; Complex; Development; Equipment; Faculty; Foundations; Funding; Grant; Image; Imaging Device; Individual; Lasers; Light; Microscope; Mission; Pattern; Pediatric Hospitals; Pediatric Research; Privatization; Publications; Qualifying; Request for Applications; Research; Research Personnel; Research Support; Resolution; Sampling; System; Technology; Three-Dimensional Imaging; Training; Universities; cell motility; cost effective; experience; instrument; instrumentation; medical schools; multi-photon; neural circuit; programs; recruit

Project Summary/Abstract: Three-dimensional imaging of large samples is becoming a vital technology for identifying complex cell migration patterns, vascular integrity, developmental cell patterning and neural circuit complexity, all essential components of research at Lewis Katz School of Medicine at Temple (LKSOM). To support these research efforts, this shared instrument application requests funds for a lightsheet microscope. This lightsheet microscope will be an important addition to the Imaging Core supported over the past 10 years by grant funding through Shriners Hospitals for Pediatric Research. The mission of the imaging core is to provide a full range of state-of-the-art imaging equipment, training, and technology at a cost-effective rate. The imaging core presently has a 2 confocal, 1 multi-photon, TIRF, stereological system, laser capture, and 3 wide-field epifluorescent scopes, with over 20 researchers at Shriners Research Center, LKSOM, and Temple University who have acknowledged this core in their publications and in over 45 federal or private foundation grants. The lightsheet microscope is an important and necessary addition to the imaging core by supporting high resolution 3D imaging not capable by our present imaging devices. It will have a fundamental and significant impact on currently PHS funded projects as well as biomedical discoveries disseminated through high impact publications. The need for a lightsheet microscope developed through interactions and collaboration with multiple investigators for the need of this technology to significantly enhance their current and future research efforts. Additionally, this shared instrument will also enhance our ability to recruit new faculty who have a critical need for 3D imaging for their research program. As with all high-end imaging instrumentation, individual labs have insufficient funds to purchase or maintain a lightsheet microscope. Therefore, it is highly relevant instrument to be shared by multiple investigators and supported by an established imaging core with 10 years of experience and funding, qualified to operate, manage and maintain this microscope.

项目摘要/摘要： 大型样本的三维成像正在成为识别的重要技术 复杂的细胞迁移模式，血管完整性，发育细胞模式和神经 电路复杂性，所有研究的基本组成部分在刘易斯·卡茨医学院 寺庙（Lksom）。为了支持这些研究工作，该共享工具应用 请求资金用于照明显微镜。这个灯表显微镜将是重要的 在过去的10年中，还通过资助通过 小儿研究的Shriners医院。成像核心的使命是提供一个完整的 以具有成本效益的速度，最先进的成像设备，培训和技术范围。 成像核心目前具有2个共聚焦，1个多光子，TIRF，立体系统，激光 捕获和3个宽场落叶范围，有20多名Shriners Research的研究人员 Center，Lksom和Temple University在其出版物中承认了这一核心 以及超过45个联邦或私人基金会赠款。 灯表显微镜是对成像芯的重要且必要的补充 支持高分辨率的3D成像，我们目前的成像设备无法使用。它将有 对当前PHS资助的项目以及生物医学的基本和重大影响 通过高影响出版物传播的发现。需要灯书 显微镜是通过与多个研究人员的互动和合作开发的 这项技术需要大大增强其当前和未来的研究工作。 此外，这种共享工具还将增强我们招募拥有的新教师的能力 对他们的研究计划的3D成像的关键需求。与所有高端成像一样 仪器，个别实验室没有足够的资金购买或维护灯表 显微镜。因此，这是高度相关的工具，由多个调查员共享 并得到拥有10年经验和资金的既定成像核心的支持， 有资格操作，管理和维护此显微镜。