Humanized Mouse Core

人性化鼠标核心

• 批准号: 10629167
• 负责人: Patrizia Caposio
• 金额: $ 30.99万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-15 至 2027-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10629167
• 关键词: AMD3100; Animal Model; CD34 gene; CSF3 gene; Cells; Chemicals; Clinical; Collaborations; Cytomegalovirus; Engraftment; Exhibits; Fetal Liver; Fetal Tissue Research; Generations; Genes; Goals; Growth; Health; Hematopoietic Stem Cell Mobilization; Hematopoietic stem cells; Human; Human Biology; Immune; In Vitro; Infection; Infrastructure; Investigation; Knowledge; Latent virus infection phase; Macrophage; Modeling; Molecular; Mus; Oregon; Progenitor Cell Engraftment; Protein Isoforms; Publishing; Reporting; Research; Research Project Grants; Role; Science; Signal Pathway; Signal Transduction; Source; Standardization; Supervision; Supporting Cell; System; Testing; Tissues; Umbilical Cord Blood; Universities; Vaccine Therapy; Viral; Virus; Virus Latency; Virus Replication; Work; cell type; design; experimental study; gene therapy; human fetus tissue; human tissue; humanized mouse; in vivo; inhibitor; mouse model; mutant; programs; reactivation from latency; small hairpin RNA; stem cells

SUMMARY - HUMANIZED MOUSE CORE (CORE A) The huNSG mouse model nicely recapitulates aspects of human biology that are not supported in other systems. This humanized mouse model also offers the only small animal model capable of supporting HCMV infection, latency, and reactivation. This PPG requires our highly standardized huNSG model in order to effectively characterize unique conditions and quantitatively differentiate viral growth and dissemination of different viral mutants and treatments. Core A will provide the infrastructure and knowledge required for completion of the proposed research projects involving huNSG mice and standardize analysis between all projects to allow for direct comparisons. By working directly with the Project leaders, Core A will aid in designing and execution of the proposed experiments.

摘要 - 人性化鼠标核心（核心 A） huNSG 小鼠模型很好地概括了其他模型不支持的人类生物学的各个方面 系统。这种人源化小鼠模型还提供了唯一能够支持 HCMV 的小动物模型 感染、潜伏期和重新激活。该 PPG 需要我们高度标准化的 huNSG 模型才能 有效地表征独特的条件并定量区分病毒的生长和传播 不同的病毒突变体和治疗方法。核心 A 将提供所需的基础设施和知识 完成涉及 huNSG 小鼠的拟议研究项目并对所有小鼠之间的标准化分析进行标准化 项目，以便进行直接比较。通过直接与项目负责人合作，核心 A 将帮助 设计和执行所提出的实验。