Deciphering dopaminergic circuits required for food anticipatory activity in mice

破译小鼠食物预期活动所需的多巴胺能回路

基本信息

批准号：
10629786
负责人：
Andrew David Steele
金额：
$ 14.7万
依托单位：
CALIFORNIA STATE POLY U POMONA
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-05-01 至 2027-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10629786
关键词：
Activity Cycles Adaptive Behaviors Address Anatomy Animals Back Behavior Behavioral Biological Process Brain Caring Circadian Rhythms Corpus striatum structure Data Dependovirus Disease Dopamine Dorsal Eating Enzymes Exhibits Food Fright Genetic Genetic Identity Health Hunger Hyperphagia Hypothalamic structure Injections Knockout Mice Light Link LoxP-flanked allele Maps Measures Medial Mediating Medical Modernization Mus Nature Neurobiology Neurons Physiological Play Population Production Rattus Recording of previous events Regulation Research Role Signal Transduction Site Societies Stimulus Substantia nigra structure System Techniques Temperature Testing Time Translational Research Tyrosine 3-Monooxygenase United States Viral Vision behavior observation calbindin circadian conditional knockout cost designer receptors exclusively activated by designer drugs dopamine transporter dopaminergic neuron experimental study feeding feeding schedule genetic manipulation grasp light entrainment nerve supply neural circuit neurotransmission response restoration retrograde transport suprachiasmatic nucleus tool translational impact

Activity Cycles Adaptive Behaviors Address Anatomy Animals Back Behavior Behavioral Biological Process Brain Caring Circadian Rhythms Corpus striatum structure Data Dependovirus Disease Dopamine Dorsal Eating Enzymes Exhibits Food Fright Genetic Genetic Identity Health Hunger Hyperphagia Hypothalamic structure Injections Knockout Mice Light Link LoxP-flanked allele Maps Measures Medial Mediating Medical Modernization Mus Nature Neurobiology Neurons Physiological Play Population Production Rattus Recording of previous events Regulation Research Role Signal Transduction Site Societies Stimulus Substantia nigra structure System Techniques Temperature Testing Time Translational Research Tyrosine 3-Monooxygenase United States Viral Vision behavior observation calbindin circadian conditional knockout cost designer receptors exclusively activated by designer drugs dopamine transporter dopaminergic neuron experimental study feeding feeding schedule genetic manipulation grasp light entrainment nerve supply neural circuit neurotransmission response restoration retrograde transport suprachiasmatic nucleus tool translational impact

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项目摘要

Project Summary/Abstract Given the over-abundance of food in modern society, it is easy to lose sight of the importance of feeding to animals in nature. For most animals, food is extremely scarce and being wise to opportunities to eat is essential for survival. As such, the circadian system has evolved to receive a number of different stimuli— i.e., light, temperature, food, and even fear—to keep biological processes coordinated and allow for adaptation to ever changing conditions. The ability to tell time relative to feeding has a long history of research, going back more than one hundred years hundred years with behavioral observations of honeybees and rats. However, the neural circuitry behind adaptation of behavior to timed feeding has continually escaped our grasp. In this project, I will utilize conditional genetics, viral restorations, and chemogenetic tools to identify the dopamine population (both anatomically and genetically) that is required for the behavioral expression of food anticipatory activity in lab mice. From here, we will build out a more comprehensive characterization of how a select population of dopamine neurons projecting to the striatum can inform the time keeping systems of the brain that food is available.

项目摘要/摘要鉴于现代社会中食物过度的过度，很容易忽视喂养的重要性自然界中的动物。对于大多数动物而言，食物极为稀缺，并且明智地吃饭的机会是生存至关重要。因此，昼夜节律已经发展为接收许多不同的刺激 - 即光，温度，食物甚至恐惧 - 保持生物过程协调并允许适应不断变化的条件。相对于喂养，分辨时间的能力具有悠久的历史研究，可以追溯到一百年以上蜜蜂和老鼠。但是，行为适应定时喂养背后的神经回路具有继续逃脱了我们的掌握。在这个项目中，我将利用条件遗传学，病毒修复和鉴定多巴胺种群的化学生成工具（在解剖学和遗传学上）是所需的用于实验室小鼠食物预期活动的行为表达。从这里，我们将建立更多综合表征多巴胺神经元如何投射到纹状体可以通知将大脑的时间保留的时间可用。