Optical Microscopy Core

光学显微镜核心

• 批准号: 10630255
• 负责人: MARK A. MC NIVEN
• 金额: $ 18.92万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630255
• 关键词: Antibodies; Bioprobe; Biosensor; Cells; Charge; Collection; Computers; Confocal Microscopy; Consult; Continuing Education; Coupled; Data Analyses; Databases; Development; Digestive System Disorders; Disease; Doctor of Philosophy; Dyes; Education; Epithelium; Equipment; Experimental Designs; Faculty; Feedback; Fluorescence; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Fluorescent Probes; Funding; Gastroenterology; Goals; Grant; Hepatology; Histology; Hour; Human Resources; Imaging technology; Individual; Infrastructure; Instruction; Laboratories; Light; Lipids; Liquid substance; Liver; Maintenance; Manuscripts; Measures; Methods; Microinjections; Microscope; Microscopic; Microscopy; Modeling; Nucleotides; Optics; Organoids; Pancreas; Photobleaching; Physiological; Preparation; Protein Dynamics; Proteins; Publications; Publishing; Reagent; Research; Research Personnel; Resources; Services; Signal Pathway; Signal Transduction; Signaling Molecule; Students; Techniques; Technology; Three-Dimensional Image; Time; Tissues; Training; cellular imaging; cost; digital; experience; fluorescence microscope; image reconstruction; imaging modality; imaging software; innovation; live cell imaging; member; multiphoton microscopy; novel; optical imaging; programs; protein protein interaction; recruit; response; superresolution microscopy; technology development; tool; user-friendly

PROJECT SUMMARY: OPTICAL MICROSCOPY CORE The objective of the C-SiG Optical Microscopy Core, is to be a state-of-the-art, user-friendly service that connects investigators with the many optical technologies and applications at a reasonable cost. Under the direction of Dr. Mark McNiven, a well-established cell biologist, the Core integrates existing resources from individual investigators in the Division of Gastroenterology and Hepatology (GIH) with new equipment added during this funding cycle (histology, live cell imaging, and lightsheet microscopes). The C-SiG Optical Microscopy Core also collaboratively partners with the Mayo Microscopy and Cell Analysis (MMCA) Core to enhance resources available to center members by providing GI-relevant expertise and training for C-SiG members. The Specific Aims of this core are three-fold. First, to provide reliable, accessible, state-of-the-art microscopic technology to all C-SiG members that will facilitate their study of GI cellular signaling cascades. Second, to educate and train C-SiG members in the use of both basic and sophisticated cellular imaging methods. Emphasis is placed on providing technical instruction as well as educating faculty on how such approaches can expand the scope and breadth of their scientific programs. Third, to develop and apply state- of-the-art optical imaging technologies, including fluorescent probes, vital dyes, and biosensors, to study GI tissues and/or cells. The most popular Core service is access to the well-maintained C-SiG Confocal Microscopes, for which utilization has more than doubled in the past 4.5 years. The Core also provides instruction, technical advice, data interpretation, and development of novel, innovative optical approaches to the study of signaling pathways in GI cells and tissues. These services cover a wide range of topics including: real-time computer/video imaging of live cells; confocal microscopy coupled with computer-based 3-D image reconstruction; Fluorescence Resonance Energy Transfer (FRET) applications to measure dynamic protein- protein interactions; Fluorescence Recovery After Photobleaching (FRAP) that allows the quantitation of protein recruitment/turnover; Fluorescence Loss in Photobleaching (FLIP); microinjection of living cells; expression and use of fluorescence-based bioprobes that facilitates the study and localization of specific signaling molecules including both proteins and lipids; the development and application of specific photo- activatable caged-compounds that allow a precise temporal and spatial activation of desired signaling molecules in live cells; Total internal reflection (TIRF) microscopy; multiphoton microscopy, and super- resolution microscopy. Over the past 4.5 years, the C-SiG Optical Microscopy Core has provided services to 55 members [47 current members (69% of current membership)] and supported 133 publications.

项目摘要：光学显微镜核心 C-SiG 光学显微镜核心的目标是成为最先进、用户友好的服务， 以合理的成本将研究人员与许多光学技术和应用联系起来。下 在知名细胞生物学家 Mark McNiven 博士的指导下，该核心整合了来自 胃肠病学和肝病学 (GIH) 部门的个体研究人员增加了新设备 在此资助周期内（组织学、活细胞成像和光片显微镜）。 C-SiG 光学 显微镜核心还与 Mayo 显微镜和细胞分析 (MMCA) 核心合作， 通过为 C-SiG 提供 GI 相关专业知识和培训，增强中心成员可用的资源 成员。该核心的具体目标有三个方面。首先，提供可靠、可访问、最先进的 为所有 C-SiG 成员提供显微技术，这将有助于他们对胃肠道细胞信号级联的研究。 其次，教育和培训 C-SiG 成员使用基本和复杂的细胞成像 方法。重点放在提供技术指导以及教育教师如何进行此类培训 方法可以扩大其科学计划的范围和广度。第三，制定和应用国家 最先进的光学成像技术，包括荧光探针、活体染料和生物传感器，用于研究胃肠道 组织和/或细胞。最受欢迎的核心服务是使用维护良好的 C-SiG Confocal 显微镜，其利用率在过去 4.5 年中增加了一倍多。核心还提供 指导、技术建议、数据解释以及新颖、创新的光学方法的开发 胃肠道细胞和组织中信号通路的研究。这些服务涵盖广泛的主题，包括： 活细胞的实时计算机/视频成像；共焦显微镜与基于计算机的 3D 图像相结合 重建;荧光共振能量转移 (FRET) 应用测量动态蛋白质 蛋白质相互作用；光漂白后的荧光恢复 (FRAP) 可定量 蛋白质招募/周转；光漂白中的荧光损失 (FLIP)；活细胞显微注射； 基于荧光的生物探针的表达和使用，有助于特定的研究和定位 信号分子，包括蛋白质和脂质；特定照片的开发与应用 可激活的笼状化合物，可以精确地在时间和空间上激活所需的信号传导 活细胞中的分子；全内反射（TIRF）显微镜；多光子显微镜和超 分辨率显微镜。在过去的 4.5 年里，C-SiG 光学显微镜核心为以下客户提供了服务： 55 名会员 [47 名现任会员（占现任会员的 69%）] 并支持 133 种出版物。