Ambystoma Genetic Stock Center

钝口螈遗传资源中心

• 批准号: 10569468
• 负责人: Stephen Randal Voss
• 金额: $ 2.05万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10569468
• 关键词: Adolescent; Adult; Ambystoma; Ambystoma mexicanum; Animals; Area; Arts; Awareness; Biological; Biomedical Research; Body part; Brain; Breeding; Businesses; Cellular biology; Collaborations; Collection; Communities; Computers; Cryopreservation; Custom; Development; Disease; Embryo; Embryonic Development; Ensure; Environment; Evolution; Funding; Future; Genes; Genetic; Genome; Genomics; Health; Heart; Hour; Human; IACUC; Informatics; Infrastructure; Institution; International; Jaw; Kentucky; Knock-out; Life; Limb structure; Louisiana; Medicine; Methods; Modeling; Natural regeneration; Neurobiology; Newsletter; Online Systems; Organ Model; Physiology; Play; Population; Practice Management; Procedures; Protocols documentation; Reproducibility; Research; Research Personnel; Research Support; Resources; Retina; Role; Salamander; Science; Shipping; Site; Spinal Cord; System; Tail; Technology; Time; TimeLine; Tissues; Transgenic Organisms; Translating; United States National Institutes of Health; Universities; Vertebrates; Work; animal resource; college; cost; experience; genetic resource; knowledge base; learning strategy; meetings; operation; organ regeneration; preservation; single-cell RNA sequencing; sperm cell; sperm cryopreservation; tissue repair; user-friendly; web site; welfare

OVERALL COMPONENT - PROJECT SUMMARY This application seeks support to continue operations of the Ambystoma Genetic Stock Center (AGSC) at the University of Kentucky (UK). The AGSC is the only federally funded resource center that distributes Mexican axolotls (Ambystoma mexicanum), a salamander that provides living materials in support of biomedical research nationally and internationally. Most notably, axolotls are unique among vertebrates in being able to regenerate numerous tissues and body parts. These include the spinal cord, limbs, jaw, retina, brain, heart, and tail, all of which can be regenerated at any point during embryonic, larval, and adult life. Also, axolotls are used by researchers that work in additional areas, including neurobiology, ecotoxicology, development, physiology, cell biology, genetics, genomics, and evolution. This is an exciting time for the axolotl as it’s incredibly large genome (32 Gb) was recently sequenced and assembled. New and assessable resources are allowing cutting edge technologies like gene-editing and single cell RNA sequencing to be performed for the first time, approaches that are rapidly accelerating discoveries using axolotl models. In just the past 4 years, NIH support for axolotl research has tripled and there has been a significant increase in transgenic and knock- out lines in the community. PI’s in the salamander community recently came together to discuss future opportunities and challenges, and the enabling role the AGSC will play in moving community efforts forward. During the previous funding period, the AGSC moved internally from the College of Arts and Sciences to the College of Medicine (COM) at UK. This move has strengthened the AGSC in several significant ways that clearly demonstrate long term commitment by the host institution for this irreplaceable resource. Specifically, funding was made available to replace re-circulating systems and create a more user friendly website to facilitate axolotl stock purchasing and more efficiently disseminate information that is useful for working with axolotls. Additionally, COM-UK has extensive experience working with university core research centers and thus provides a more stable administrative environment to support AGSC business activities and to better ensure regulatory compliance and animal health and welfare. Over the next 5 years, the AGSC will build upon current momentum by maintaining and generating high quality, axolotl stocks that are needed by a growing number of NIH-funded investigators. Husbandry and management practices will be modified to increase availability of post-embryonic stocks that are seeing increasing demand by researchers. Also, transgenic and knock-out stocks will be prioritized for import into the AGSC and cryopreservation methods will be developed to preserve and more efficiently manage stocks. The AGSC will continue to serve as an informatics hub where investigators obtain information about the collection, technical procedures, potential collaborators, and research findings. Overall, the project will ensure distribution and long-term sustainability of axolotl resources to NIH-funded investigators. !

总体组件 - 项目摘要 该申请寻求支持继续在Ambystoma遗传储备中心（AGSC）的运营 肯塔基大学（英国）。 AGSC是唯一分发墨西哥的联邦资助资源中心 Axolotls（Ambystoma Mexicanum），一种提供生物医学的活材料的sal 在国内和国际上进行研究。最值得注意的是，在能够 再生许多组织和身体部位。其中包括脊髓，四肢，下颌，视网膜，大脑，心脏， 和尾巴，所有这些都可以在胚胎，幼虫和成人生活期间的任何时刻再生。另外，Axolotls是 在其他领域工作的研究人员，包括神经生物学，生态学，发展， 生理，细胞生物学，遗传学，基因组学和进化。对于Axolotl来说，这是一个令人兴奋的时刻 最近对大型基因组（32 GB）进行了测序并组装。新的和可评估的资源是 允许对基因编辑和单细胞RNA测序等尖端技术进行 第一次，使用Axolotl模型快速加速发现的方法。在过去的四年中， NIH对Axolotl研究的支持增加了两倍，转基因和敲门率显着增加 淘汰社区。 PI的Salamander社区最近聚集在一起讨论未来 机遇和挑战，以及AGSC将在推动社区努力前进中发挥的作用。 在上一个资金期间，AGSC内部从艺术与科学学院移动到 英国医学院（COM）。这一举动以几种重要的方式加强了AGSC 清楚地证明了东道机构对这种不可替代的资源的长期承诺。具体来说， 资金可用于替换重新循环系统，并创建一个更具用户友好的网站 促进Axolotl股票的购买，并更有效地传播可用于合作的信息 Axolotls。此外，COM-UK在大学核心研究中心和 因此，提供了一个更稳定的行政环境，以支持AGSC业务活动并改善 确保监管合规性以及动物健康和福利。在接下来的5年中，AGSC将基于 通过维护和产生高质量的Axolotl股票，这是当前的动力 NIH资助的调查人员数量。畜牧业和管理实践将被修改以增加 可获得研究人员需求不断增加的胚胎后股票。另外，转基因和 淘汰库存将优先考虑到AGSC，将开发冷冻保存方法 保存和更有效地管理股票。 AGSC将继续作为信息枢纽 调查人员获得有关收集，技术程序，潜在合作者以及的信息 研究结果。总体而言，该项目将确保Axolotl资源的分配和长期可持续性 致国立卫生研究院资助的调查人员。 呢