ShEEP Request for iNSCOPIX nVue System

SheEEP 对 iNSCOPIX nVue 系统的请求

• 批准号: 10534510
• 负责人: Priyattam J. Shiromani
• 金额: --
• 依托单位: RALPH H JOHNSON VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10534510
• 关键词: Abnormal Cell; Address; Affect; Alcohol consumption; Algorithms; Animals; Area; Behavior; Biological; Biosensor; Blood flow; Brain; Brain Injuries; Brain imaging; Calcium; Cells; Collaborations; Computer software; Data; Data Analyses; Data Store; Devices; Disease; Equipment; Female; Fluorescence; Fright; Funding; Genetic Engineering; Goals; Image; Individual; Joints; Manufacturer Name; Maps; Measures; Medical center; Microscope; Monitor; Motion; Mus; Nerve Degeneration; Neurodegenerative Disorders; Neuroglia; Neurons; Neurosciences; Output; Pathologic; Pattern; Post-Traumatic Stress Disorders; Productivity; Request for Applications; Research; Research Personnel; Rodent; Sample Size; Services; Sheep; Signal Transduction; Special Equipment; Substance abuse problem; System; Technology; Time; Training; Translational Research; Traumatic Brain Injury; Vendor; Visualization; analog; brain cell; brain circuitry; cell type; data acquisition; denoising; digital; fluorophore; independent component analysis; indexing; instrumentation; interest; lens; male; miniaturize; neurotransmitter release; peer; response; sex; solid state; student training; substance use; synergism; traumatic stress; user-friendly

The brain is no longer considered a “black box” because new devices and genetically- engineered biosensors have made it possible to monitor activity of cells deep in the brain. However, the brain is comprised of many different types of cells, and a device is needed that monitors activity of different types of cells adjacent to each other especially in animals actively engaged in behavior. Over the last year a new breakthrough technology was made available - a miniature microscope that weighs only 2g, makes it possible to monitor activity of two different cells in freely-behaving rodents. This device identifies activity patterns of brain cells during specific behaviors, allowing investigators to construct brain activity maps of specific brain circuits regulating purposeful behavior. Researchers can determine how neurodegenerative disease or specific conditions such as traumatic brain injury or substance abuse affects circuit function. The nVue miniscope system enables investigators to identify activity at the single cell level and how dysfunctional activity at the local level can cause pathological behavior. However, the necessary core instrumentation that would serve to measure critical indices of cell signaling, quantification and imaging, are not currently available to an individual investigator at RHJ VAMC. The instrumentation requested in this proposal will directly address this issue. The underlying hypothesis of this proposal is that abnormal cell signaling in specific brain circuits leads to pathological behavior. A device is needed to monitor activity of brain circuits. This equipment will accomplish the following specific aims: i) image brain circuits activated during purposeful behavior, and ii) identify change in circuit activity in response to disease, substance abuse, and traumatic stress or brain injury. By mapping activity in local circuits it will be possible to reestablish activity and restore normal behavior.

大脑不再被认为是“黑匣子”，因为新设备和一般的设备 - 工程化的生物传感器使监测大脑深处细胞的活动成为可能。 但是，大脑由许多不同类型的单元组成，需要一个设备 监视彼此相邻的不同类型细胞的活性，尤其是在动物中 从事行为。在过去的一年中，采用了新的突破性技术 - 仅加权2G的微型显微镜，可以监视两个不同的活动 自由行为的啮齿动物中的细胞。该设备确定了脑细胞的活动模式 特定的行为，使研究人员能够构建特定大脑的大脑活动图 电路降低有目的的行为。研究人员可以确定神经退行性 疾病或特定疾病（例如脑外伤或药物滥用）会影响电路 功能。 NVUE Miniscope系统使研究人员能够识别单个单元的活动 水平以及在地方一级的功能失调的活性如何引起病理行为。然而， 必要的核心仪器，以测量细胞信号的关键指标， 数量和成像目前尚不适用于RHJ的个别研究者 vamc。本提案中要求的仪器将直接解决此问题。 该提议的基本假设是特定的细胞信号异常 脑电路导致病理行为。需要设备来监视大脑的活动 电路。该设备将完成以下特定目的：i）图像脑电路 在有目的的行为期间激活，ii）确定电路活动的变化 疾病，药物滥用和创伤性压力或脑损伤。通过在本地绘制活动 电路将有可能重新建立活动并恢复正常行为。