Investigating the effects of adolescent binge drinking on prelimbic somatostatin circuitry

研究青少年酗酒对边缘前生长抑素回路的影响

• 批准号: 10535596
• 负责人: Avery Sicher
• 金额: $ 3.79万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10535596
• 关键词: Adolescence; Adolescent; Adult; Affect; Age; Agonist; Alcohol abuse; Alcohol consumption; Alcohol dependence; Alcohols; Animal Model; Animals; Behavioral; Blood; Calcium Signaling; Cells; Closure by clamp; Clozapine; Consumption; Development; Electrophysiology (science); Fiber; Goals; Heavy Drinking; Intervention; Lead; Life; Link; Modeling; Mus; Neurons; Oxides; Pattern; Pharmacological Treatment; Photometry; Population; Public Health; Reporting; Risk; Risk Factors; Role; Somatostatin; Synapses; Techniques; Virus; Vulnerable Populations; adolescent alcohol effect; adolescent alcohol exposure; adolescent alcohol misuse; adolescent binge drinking; alcohol content; alcohol effect; alcohol exposure; alcohol risk; alcohol use disorder; binge drinking; designer receptors exclusively activated by designer drugs; drinking; early adolescence; effective intervention; experimental study; in vivo; neuroadaptation; neuronal excitability; neuropsychiatry; patch clamp; substance use; therapeutic target; underage drinking; voltage clamp

PROJECT ABSTRACT Binge drinking during adolescence has been identified as a potential risk factor for alcohol use disorder (AUD) in adulthood. Despite this, the precise neuronal populations which are altered by adolescent drinking, and which may contribute to changes in adulthood drinking, have not been fully characterized. Somatostatin (SST)- expressing neurons in the prelimbic (PL) cortex are altered by adult binge drinking in animals models, and manipulating the activity of these neurons can reduce binge drinking. The goal of this proposal is to determine the role of SST neurons following adolescent binge drinking, as well as during escalated adulthood alcohol consumption following adolescent exposure. The first set of experiments will use whole-cell patch-clamp electrophysiology and fiber photometry to understand how adolescent alcohol use affects SST neurons in the PL cortex. The second set of experiments will use behavioral and chemogenetic manipulations to identify a causal role for SST neurons in changes in adulthood alcohol consumption after adolescent binge drinking.

项目摘要 青春期的暴饮暴食已被确定为酒精使用障碍的潜在危险因素（AUD） 成年。尽管如此，通过青少年饮酒而改变的精确神经元种群 可能导致成年饮酒的变化，尚未充分表征。生长抑素（SST） - 在动物模型中成人暴饮暴食和 操纵这些神经元的活性可以减少暴饮暴食。该提议的目的是确定 SST神经元在青少年暴饮暴食后以及成年期间的饮酒期间的作用 青少年接触后的消费。第一组实验将使用全细胞贴片钳 电生理学和纤维光度法了解青少年酒精的使用如何影响PL中的SST神经元 皮质。第二组实验将使用行为和化学遗传操纵来识别因果关系 SST神经元在青少年暴饮暴食后成年饮酒变化中的作用。