Biophysics Core

生物物理学核心

基本信息

批准号：
10650878
负责人：
Gregory B Melikian
金额：
$ 80.21万
依托单位：
SEATTLE CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-22 至 2027-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10650878
关键词：
Acylation Automobile Driving Beds Behavior Biological Biological Models Biophysics Capsid Cells Cellular Structures Complex Coupled Detection Development Educational workshop Environment Equipment Event Fluorescence Fluorescence Microscopy Fluorescence Resonance Energy Transfer Goals HIV HIV-1 Hydroxyl Radical Image In Vitro Investigation Joints Label Life Cycle Stages Machine Learning Macromolecular Complexes Mass Spectrum Analysis Membrane Proteins Mission Nuclear Import Nucleic Acids Ohio Persons Phase Photobleaching Primer Extension Process Protein Dynamics Proteins RNA RNA Probes Research Personnel Resources Services Spectrum Analysis Techniques Technology Testing Training Universities Viral Visit Visualization biophysical analysis biophysical properties biophysical techniques cellular imaging crosslink insight instrumentation new technology novel imaging technology particle protein complex quantitative imaging response single molecule stoichiometry structural biology superresolution imaging tool videoconference

项目摘要

ABSTRACT, CORE 3 Biophysical methods, such as single-molecule techniques, quantitative imaging/spectroscopy and native mass spectrometry (nMS) are powerful tools to gain mechanistic insights into key dynamic interactions and processes occurring during the early and late steps of the HIV-1 lifecycle. The goal of Core 3 (Biophysics Core) is to provide requisite instrumentation, expertise and training to augment the B-HIVE capabilities across all three center projects by enabling cutting-edge studies of protein-protein, protein-nucleic acid and protein-membrane interactions in living cells and in model systems. To accomplish these goals we will: (1) Provide advanced fluorescence microscopy capabilities to visualize early and late events of the HIV life cycle (Melikian); (2) provide advanced capabilities in single-molecule biophysics to probe dynamics of proteins, nucleic acids and their complexes (Millar & Musier-Forsyth); and (3) provide methods for biophysical characterization of macromolecular complexes (Musier-Forsyth and Wysocki). A critical part of the Core 3 mission is training the users remotely or in person, through visits to the labs of the Core co-leads, workshops and videoconferences. To accomplish this goal, the core will provide basic imaging, single-molecule and nMS services using established pipelines to all B-HIVE projects, as needed, implement advanced techniques and engage in long-term projects through collaborative efforts with the B-HIVE investigators, and develop novel technologies in response to the needs of Projects 1-3. 1

摘要，核心3 生物物理方法，例如单分子技术，定量成像/光谱和天然质量光谱法（NMS）是强大的工具发生在HIV-1生命周期的早期和晚期。核心3（生物物理学核心）的目标是提供必要的仪器，专业知识和培训，以增强所有三个中心的B hive功能通过实现蛋白质蛋白质，蛋白质核酸和蛋白质 - 膜的尖端研究活细胞和模型系统中的相互作用。为了实现这些目标，我们将：（1）提供高级荧光显微镜能力可视化HIV生命周期的早期和晚期事件（Melikian）；（2）提供单分子生物物理学的晚期能力可探测蛋白质，核酸及其的动力学复合体（Millar＆Musier-Forsyth）；（3）提供了生物物理表征的方法大分子复合物（Musier-Forsyth和Wysocki）。核心3任务的关键部分是培训用户远程或亲自访问核心共同领导，研讨会和视频会议的实验室。为了实现这一目标，核心将使用已建立的基本成像，单分子和NMS服务根据需要，所有B-HY项目的管道实施高级技术并参与长期项目通过与B-hive调查人员的合作努力，并开发出新的技术来应对项目1-3的需求。 1