Arcturus XT-TI Laser Capture Microdissection Instrument

Arcturus XT-TI 激光捕获显微切割仪器

• 批准号: 8948705
• 负责人: JAMES Richard GOLDENRING
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-01-01 至 2015-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8948705
• 关键词: 10 year old; Academic Medical Centers; Address; Back; Biological Markers; Cancer Biology; Cell membrane; Cells; Charge; Computer software; Core Facility; Data; Diabetes Mellitus; Disease; Dissection; Equipment; Evaluation; Fee-for-Service Plans; Fluorescence; Fluorescent Probes; Fluorescent in Situ Hybridization; Freezing; Funding; Gastroenterology; Healthcare Systems; Histology; Image; Image Analysis; Immunofluorescence Immunologic; Individual; Investigation; Lasers; Manuals; Methods; Microscope; Modality; Neoplasm Circulating Cells; Nuclear; Paraffin; Population; Process; Proteins; Reporter; Research; Research Personnel; Resolution; Resource Sharing; Resources; Scanning; Secure; Services; Slide; Specimen; Speed; Staining method; Stains; System; Tennessee; Time; Tissue Sample; Tissues; Training; Translational Research; Update; angiogenesis; base; digital; digital imaging; imaging system; instrument; instrumentation; laser capture microdissection; medical schools; meetings; mouse model; public health relevance; quantitative imaging; research study; web site

 DESCRIPTION (provided by applicant): Laser Capture Microdissection (LCM) allows rapid and accurate isolation of discrete cell populations from sections of histological tissues. The present Arcturus Veritas LCM instrument located at Vanderbilt was obtained in 2006 through combined internal resources and has been utilized by VA and Vanderbilt investigators in a variety of investigations in cancer biology, gastroenterology and diabetes research. The present Arcturus instrument is no longer eligible for service and is now non-functional. We are therefore seeking to replace the present instrument with an updated Arcturus XT-TI LCM system. In addition to replacing the capacities of the previous instruments, the present system has a second UV cutting laser that allows rapid isolation of larger tissue regions. In addition, the new instrument allows LCM on fluorescent cells from tissue samples. This capability is important for dissection of cells from an expanding number of mouse models expressing fluorescent reporter proteins and for dissection of lineages after immunostaining. In addition, the new instrument automatically focuses the laser. Previous systems have required manual realignment and focusing by a service technician. The access to the LCM instrument will be facilitated through the Digital Histology Shared Resource (DHSR) at Vanderbilt on an hourly fee-for-service basis. DHSR staff will provide LCM training as well as frozen and paraffin sectioning assistance as necessary. The DHSR also maintains supplies of LCM consumables for investigators, with appropriate charge back. This upgraded LCM capacity will allow VA investigators uninterrupted access to LCM capabilities in support of their Merit Review- funded projects.

 描述（由申请人提供）： 激光捕获显微切割 (LCM) 可以从组织学组织切片中快速准确地分离离散细胞群。位于范德比尔特的现有 Arcturus Veritas LCM 仪器于 2006 年通过内部资源联合获得，并已被 VA 和范德比尔特研究人员用于各种领域。癌症生物学、胃肠病学和糖尿病研究领域的研究中，现有的 Arcturus 仪器不再符合使用条件，因此目前无法使用。 Arcturus XT-TI LCM 系统除了取代以前仪器的功能外，还具有第二个紫外切割激光器，可以快速隔离较大的组织区域。此外，新仪器还可以在荧光细胞上进行 LCM。 这种功能对于从表达荧光报告蛋白的越来越多的小鼠模型中进行细胞解剖以及在免疫染色后进行谱系解剖非常重要。此外，以前的系统需要手动重新对准和聚焦。由服务技术人员提供 LCM 仪器的使用将通过范德比尔特的数字组织学共享资源 (DHSR) 进行，按小时收费 DHSR 工作人员将提供 LCM 培训以及冷冻和冷冻。 DHSR 还为研究人员提供 LCM 耗材供应，并提供适当的退款。升级后的 LCM 容量将使 VA 研究人员能够不间断地使用 LCM 功能，以支持其绩效审查资助的项目。