Novel coated nanostructured implant surfaces to increase osseointegration and decrease peri-implantitis in a physiologic rat model

新型涂层纳米结构种植体表面可增加生理大鼠模型中的骨整合并减少种植体周围炎

• 批准号: 10645782
• 负责人: Josephine F. Esquivel-Upshaw
• 金额: $ 65万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-21 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10645782
• 关键词: Address; Adhesions; Animal Model; Anodes; Anti-Bacterial Agents; Apoptosis; Bacteria; Bacterial Adhesion; Bone Growth; Bone Tissue; Cell Wall; Cells; Ceramics; Cervical; Charge; Chemicals; Consensus; Corrosion; Corrosives; Custom; Data; Dental; Dental Implants; Dental crowns; Deposition; Development; Diabetes Mellitus; Disease; Disease Progression; Educational workshop; Effectiveness; Etiology; Europe; Evaluation; Extravasation; Failure; Fracture; Funding; Future; Goals; Grant; Histologic; Human; Implant; Implantation procedure; In Vitro; Incidence; Industry; Infection; Inflammation; Inflammatory; Investments; Lead; Link; Maintenance; Mediating; Modality; Modeling; Modification; Mucositis; Mucous Membrane; Nanostructures; Nitrogen; Oral health; Oryctolagus cuniculus; Osseointegration; Osteoblasts; Osteogenesis; Outcome; Outcome Study; Oxides; Pathogenesis; Patients; Periodontal Diseases; Periodontitis; Physiological; Plasma Enhancement; Positioning Attribute; Prevalence; Prevention; Prevention strategy; Prognosis; Proliferating; Property; Prosthesis; Public Health; Rattus; Reaction; Recording of previous events; Recurrence; Regimen; Research; Research Project Grants; Residual state; Resistance; Risk Factors; Role; Smoking; Structure; Surface; Technology; Testing; Tissues; Titanium; Tooth structure; Translational Research; United States; Visualization; alveolar bone; bone; bone loss; cost; design; effective therapy; effectiveness evaluation; experimental study; implant design; improved; in vivo; keratinization; microbial; microbiome; nano; novel; osteoblast proliferation; particle; peri-implantitis; prevent; restoration; silicon carbide; soft tissue; standard of care; success; vapor

PROJECT SUMMARY/ABSTRACT: This R01 competing resubmission renewal is focused on establishing the efficacy of nanostructure technology with a protective coating in increasing osseointegration, and minimizing bacterial proliferation and corrosion around titanium dental implants. This is a continuation of our current grant, which developed and optimized a coating for corrosion resistance on ceramic with the goal of producing fracture-resistant restorations. Our data demonstrated excellent corrosion resistance, anti-bacterial and bone forming properties of an optimized silicon carbide (SiC) coating and this competing renewal will provide an expanded application for this coating with dental implants. The long-term goal of this research is to develop an implant which will increase osseointegration and prevent peri-implantitis to improve long-term restoration outcomes. The overall objective is to critically evaluate the anti-corrosive, anti-bacterial and bone integration properties of dental implants with a pioneering design that will incorporate nanostructured topography and protective coatings, which we developed in the previous funding cycle, using a rat model that will physiologically simulate the etiology of peri-implantitis. This unique, translational research project will employ the use of a rat model with physiologically induced bacterial-mediated peri-implantitis. A combination of nanostructure technology through anodization of the titanium surface, customized for osteofunctional and anti-bacterial efficacy, will be applied in conjunction with protective coatings (Silicon Carbide and Quaternized Silicon Carbide) developed during the last funding cycle. This combination is expected to potentiate the osseointegrative, anti-bacterial and anti-corrosive properties of both surface modifications to create a predictable implant restoration. Initial in vitro experiments will be conducted to determine ideal nanostructure surface for osseointegration. We will coat these customized nanostructures to develop a new implant design for placement in animal models to determine osseointegration, anti-bacterial and anti-corrosive properties using state of the art nanoCT visualization of the in vivo pathogenesis of peri-implantitis. We propose the following aims to test our central hypothesis: Aim 1: To optimize nanostructure design for increasing osseointegrative potential on Ti surfaces in vitro; Aim 2: To optimize the effect of customized nanostructured coated implant surfaces on bacterial adhesion and proliferation, and corrosion in vitro; Aim 3: To determine in vivo the rate and amount of osseointegration in implants with customized nanostructure topography during early and late stage implant placement in rabbits using stability evaluation, nano-CT evaluation and histological analysis; Aim 4: To determine the effectiveness of customized nanostructured coated implants in osseointegration and prevention of peri-implantitis in vivo in the rat model of polymicrobial peri- implantitis, which physiologically mimics human peri-implantitis.

项目摘要/摘要： R01竞争重新提交的续订重点是建立纳米结构技术的功效 使用保护性涂层，以增加骨整合，并最大程度地减少细菌增殖和腐蚀 围绕钛牙齿植入物。这是我们目前赠款的延续，该赠款开发和优化了 在陶瓷上具有耐腐蚀性的涂层，目的是产生抗裂缝的修复体。我们的数据 表现出极好的耐腐蚀性，优化硅的抗细菌和骨形成特性 碳化物（SIC）涂料和这种竞争的更新将为这种涂层提供扩展的应用 植入物。这项研究的长期目标是开发一种植入物，该植入物将增加骨整合和 防止种植体植入炎，以改善长期恢复结果。总体目标是批判性评估 具有开拓性设计的牙科植入物的抗腐蚀性，抗菌和骨整合特性 我们将结合纳米结构的地形和保护涂料，我们在以前的资金中开发了这些涂料 循环，使用大鼠模型，该模型将在生理上模拟植物周围炎的病因。 这个独特的转化研究项目将采用与生理诱导的大鼠模型的使用 细菌介导的种植体炎性炎。通过阳极氧化纳米结构技术的结合 钛表面是针对骨功能和抗细菌功效定制的，将与 在最后一个融资周期中，生长了防护涂层（碳化硅和五碳化碳化硅）。 这种组合有望增强骨整合性，抗细菌和抗腐败特性 两种表面修饰以创建可预测的植入物修复。将进行初始体外实验 确定用于骨整合的理想纳米结构表面。我们将涂上这些定制的纳米结构 开发一种新的植入物设计，用于在动物模型中放置以确定骨整合，抗菌和 抗腐蚀性特性，使用植入术的体内发病机理的纳米纳克可视化状态。 我们提出以下旨在测试我们的中心假设的目的：目标1：优化纳米结构设计 在体外提高Ti表面上的骨整合潜力；目标2：优化定制的效果 纳米结构涂层的植入物表面在细菌粘附和增殖上，并在体外腐蚀；目标3： 确定带有定制纳米结构的植入物中骨整合的速率和数量 使用稳定性评估，纳米CT的早期和晚期植入物放置的地形 评估和组织学分析；目标4：确定定制纳米结构涂层的有效性 植入骨整合和预防体内植入体炎的大鼠模型的多因素周期 植入炎，生理上模仿人类植入体炎。

项目成果

Properties of SiCN Films Relevant to Dental Implant Applications.

• DOI: 10.3390/ma16155318
• 发表时间: 2023-07-28
• 期刊: MATERIALS
• 影响因子: 3.4
• 作者: Xia, Xinyi;Chiang, Chao-Ching;Gopalakrishnan, Sarathy K.;Kulkarni, Aniruddha V.;Ren, Fan;Ziegler, Kirk J.;Esquivel-Upshaw, Josephine F.
• 通讯作者: Esquivel-Upshaw, Josephine F.

Enhancing the Hydrophobicity and Antibacterial Properties of SiCN-Coated Surfaces with Quaternization to Address Peri-Implantitis.

• DOI: 10.3390/ma16175751
• 发表时间: 2023-08-22
• 期刊: Materials (Basel, Switzerland)