Cyclic nucleotide phosphodiesterase regulation in vascular calcification

血管钙化中环核苷酸磷酸二酯酶的调节

基本信息

  • 批准号:
    10641917
  • 负责人:
  • 金额:
    $ 62.97万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-06-15 至 2026-05-31
  • 项目状态:
    未结题

项目摘要

PROJECT SUMMARY Arterial calcification results from the deposition of calcium hydroxyapatite crystals in the vessel wall. It is highly prevalent in patients with chronic kidney disease (CKD) and diabetes as well as those with peripheral artery disease (PAD). When located in the arterial media, calcification is strongly associated with increased cardiovascular morbidity and mortality. Calcification is a highly regulated process controlled by a series of endogenous stimulators and inhibitors. Elevated phosphate levels can induce osteogenic transformation of vascular smooth muscle cells (SMCs). These cells release extracellular matrix-degrading enzymes including the matrix metalloproteinases (MMPs) that contribute to calcification. Despite significant progress, arterial calcification continues to be poorly understood and no useable drugs to prevent or treat it have been developed. For this reason, the process of identifying novel molecular targets, and developing therapies to target them are critical for improving outcomes in our vascular patients. The second messenger cyclic nucleotides cAMP and cGMP play important regulatory roles in a variety of human diseases that are controlled by distinct cyclic nucleotide PDE isozymes that have proven to be ideal and feasible drug targets for the treatment of human diseases. The function and regulation of PDEs in arterial calcification, however, remains unknown. This proposal is based on our preliminary data showing that phosphodiesterase 1 0A (PDE10A) is the most highly induced isoform among all PDE genes in a rodent calcification model. PDE10A is also markedly increased in calcifying VSMCs in vitro, calcified arteries in vivo, and calcified human tibial arteries from patients with PAD. Knockdown and inhibition of PDE10A significantly attenuate phosphate-induced VSMC osteogenic transformation and calcification in vitro, and deficiency of PDE10A reduces arterial calcification in vivo. Using bioinformatics analyses and a loss-of-function strategy, we have shown that the MMP family member 3 (MMP- 3, stromelysin-1) can be regulated by PDE10A in VSMCs cultured in a calcification medium. Our further preliminary mechanistic results have shown that both knockdown and inhibition of PDE1 0A block p38 MAPK activation in VSMCs during calcification. We have additionally found that inhibition of p38 MAPK attenuates MMP-3 upregulation under calcifying conditions. In this project, we propose that PDE10A mediates arterial calcification by regulating p38 MAPK-MMP-3 signaling. In this series of experiments, we will establish the role of PDE10A in arterial calcification and provide insights into the potential use of PDE10A inhibition strategies to reduce calcification in patients with CKD and PAD. Our aims are to 1) investigate the actions of PDE10A in vascular SMC osteogenic transformation and arterial calcification, 2) examine the therapeutic potential of PDE10A inhibition in arterial calcification, and 3) assess the significance of p38-MAPK-MMP-3 signaling in PDE10A-mediated vascular calcification.
项目摘要 动脉钙化是由羟基磷灰石晶体在血管壁中的沉积而产生的。这是高度的 患有慢性肾脏疾病(CKD)和糖尿病的患者以及周围动脉的患者流行 疾病(PAD)。当位于动脉介质中时,钙化与增加密切相关 心血管发病率和死亡率。钙化是一个受高度调节的过程,由一系列 内源性刺激剂和抑制剂。升高的磷酸盐水平可以诱导成骨转化 血管平滑肌细胞(SMC)。这些细胞释放细胞外基质降解酶,包括 有助于钙化的基质金属蛋白酶(MMP)。尽管取得了重大进展,但动脉 钙化仍然是对预防或治疗的可用药物的理解,已经开发出来。 因此,鉴定新的分子靶标的过程以及开发靶向目标的疗法的过程是 对于改善血管患者的预后至关重要。第二个信使循环核苷酸营地和 CGMP在各种由不同环节控制的人类疾病中起重要的调节作用 核苷酸PDE同工酶已被证明是理想的和可行的药物靶标 疾病。然而,PDE在动脉钙化中的功能和调节仍然未知。这 建议基于我们的初步数据,表明磷酸二酯酶1 0a(PDE10A)是最高的 啮齿动物钙化模型中所有PDE基因中诱导的同工型。 PDE10A也明显增加 在体外钙化VSMC,体内钙化动脉,并从PAD患者中钙化人胫骨动脉。 PDE10A的敲低和抑制显着减弱了磷酸盐诱导的VSMC成骨剂 体外的转化和钙化,PDE10A的缺乏降低了体内动脉钙化。使用 生物信息学分析和功能丧失策略,我们已经表明MMP家族成员3(MMP- 3,Stromelysin-1)可以在钙化培养基中培养的VSMC中受PDE10A调节。我们的进一步 初步机械结果表明,PDE1 0A块P38 MAPK的敲低和抑制 钙化过程中VSMC的激活。我们还发现,抑制p38 mapk衰减 MMP-3在钙化条件下上调。在这个项目中,我们建议PDE10A介导动脉 通过调节p38 MAPK-MMP-3信号传导钙化。在这一系列实验中,我们将确定角色 PDE10A在动脉钙化中的of of pde10a抑制策略的潜在用途提供了见解 减少CKD和PAD患者的钙化。我们的目的是1)调查PDE10A在 血管SMC成骨转化和动脉钙化,2)检查的治疗潜力 PDE10A抑制动脉钙化,3)评估p38-MAPK-MMP-3信号在 PDE10A介导的血管钙化。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
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Yujun Cai其他文献

Yujun Cai的其他文献

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{{ truncateString('Yujun Cai', 18)}}的其他基金

Cyclic nucleotide phosphodiesterase regulation in vascular calcification
血管钙化中环核苷酸磷酸二酯酶的调节
  • 批准号:
    10521675
  • 财政年份:
    2022
  • 资助金额:
    $ 62.97万
  • 项目类别:

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Cyclic nucleotide phosphodiesterase regulation in vascular calcification
血管钙化中环核苷酸磷酸二酯酶的调节
  • 批准号:
    10521675
  • 财政年份:
    2022
  • 资助金额:
    $ 62.97万
  • 项目类别:
Arterial Medial Calcification and Cell Differentiation
动脉中层钙化和细胞分化
  • 批准号:
    7920600
  • 财政年份:
    2009
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  • 项目类别:
Arterial Medial Calcification and Cell Differentiation
动脉中层钙化和细胞分化
  • 批准号:
    7261506
  • 财政年份:
    2007
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  • 项目类别:
Arterial Medial Calcification and Cell Differentiation
动脉中层钙化和细胞分化
  • 批准号:
    7805556
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    2007
  • 资助金额:
    $ 62.97万
  • 项目类别:
Arterial Medial Calcification and Cell Differentiation
动脉中层钙化和细胞分化
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    8056615
  • 财政年份:
    2007
  • 资助金额:
    $ 62.97万
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