Host and parasite factors influencing P. vivax RBC invasion and asexual development

影响间日疟原虫红细胞侵袭和无性发育的宿主和寄生虫因素

基本信息

批准号：
10641026
负责人：
David Serre
金额：
$ 70.63万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-08 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10641026
关键词：
Antibodies Antigens Area Binding Biology Blood Blood specimen Cambodia Cambodian Cell Cycle Progression Cell physiology Cell surface Cells Cellular Indexing of Transcriptomes and Epitopes by Sequencing Characteristics Collaborations Complement DNA Data Development Erythrocytes Exposure to Foundations G6PD gene Gene Expression Gene Expression Profile Gene Expression Profiling Genetic Polymorphism Genomic approach Genotype Geographic Distribution Glucosephosphate Dehydrogenase Deficiency Growth Hemoglobin Hemoglobinopathies Human Immune Immunity In Vitro Individual Infection Inflammation Invaded Leukocytes Malaria Malaria Vaccines Measures Molecular Parasitemia Parasites Participant Pathway interactions Patients Peripheral Blood Mononuclear Cell Phenotype Plasmodium Plasmodium vivax Population Predisposition Process Proteins RNA Regulation Role Sampling Serology Severity of illness Sex Differentiation Solid Symptoms Testing Transcriptional Regulation Variant Vivax Malaria Whole Blood acquired immunity asexual clinically relevant cytokine density experience experimental study genome-wide human pathogen improved in vivo insight novel parasite invasion patient subsets prevent resilience response single-cell RNA sequencing transcriptome transcriptome sequencing transcriptomics vaccine trial

项目摘要

Abstract Plasmodium vivax threatens half of the world’s population and is surprisingly resilient to on-going malaria elimination efforts, partially due to its unique characteristics and our poor understanding of its biology. Here, we propose to conduct state-of-the-art transcriptomic analyses using materials derived directly from Cambodian patients to rigorously assess the importance of red blood cell polymorphisms, host immunity and parasite factors in modulating the efficacy of red blood cell invasion and P. vivax intraerythrocytic development. Our studies will not only provide a better understanding of the fundamental molecular and cellular processes underlying blood stage P. vivax infections but will also provide a solid foundation to develop better malaria vaccines against this important but understudied human pathogen.

抽象的疟原虫Vivax威胁到世界人口的一半，令人惊讶地有弹性消除努力，部分原因是它的独特特征和我们对其生物学的不良理解。在这里，我们提议使用直接衍生自柬埔寨的材料进行最先进的转录组分析患者严格评估红细胞多态性，宿主免疫学和寄生虫因子的重要性在调节红细胞浸润和腹腔内发育的效率时。我们的研究将不仅可以更好地理解血液基本过程的基本分子和细胞过程阶段P. Vivax感染，但也将为此类提供稳固的基础来开发更好的疟疾疫苗重要但了解人类病原体。