LUMICKS's C-Trap

LUMICKS 的 C 型陷阱

• 批准号: 10641464
• 负责人: Arne Gennerich
• 金额: $ 60万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10641464
• 关键词: Acceleration; Applications Grants; Area; Award; Biological; Biological Process; Biophotonics; Cancer Center; Chromatin; Collaborations; Color; Consultations; DNA; DNA Repair; DNA biosynthesis; Faculty; Fluorescence; Fluorescence Microscopy; Funding; Genetic Transcription; Goals; Health; Human; Immunology; Investigation; Malignant Neoplasms; Measurement; Medicine; Microfluidics; Microtubules; Names; Nature; Nobel Prize; Pathway interactions; Phase; Physics; RNA Editing; RNA replication; RNA-Protein Interaction; Regulation; Research; Research Personnel; System; Technology; Testing; Training; Transcriptional Regulation; Translations; United States National Institutes of Health; Writing; anticancer research; cancer therapy; cell motility; college; confocal imaging; innovation; instrument; invention; laser tweezer; mechanotransduction; molecular imaging; novel; optic tweezer; single molecule; therapeutic candidate

Project Summary The goal of this proposal is to acquire a state-of-the-art optical tweezers instrument combined with multi-color single-molecule imaging for the recently created Core for Single-Molecule Analysis (CSMA) within the Gruss- Lipper Biophotonics Center (GLBP) at the Albert Einstein College of Medicine (Einstein). Specifically, we propose to make LUMICKS’ C-Trap (dual optical tweezers combined with 3-color confocal imaging and multi-channel microfluidics) available to the researchers of Einstein through expert training, consultation and collaborations. Due to the integration of optical tweezers with multi-color fluorescence microscopy, the C-Trap allows correlative force (mechanosensing) and fluorescence measurements to be performed, enabling investigation of a wide range of biological processes, including single-molecule studies on DNA- and RNA-protein interactions (transcription, DNA and RNA replication, and DNA repair), translation, proteasomal degradation, phase separation, and immunology, to name a few areas of research. Placement of the C-Trap in the CSMA will greatly enhance and accelerate NIH-funded projects at Einstein, many of which are ideal for single-molecule studies and would greatly benefit from access to this technology. In particular, the C-Trap will be used to study the regulation of the microtubule transport system, chromatin organization and function, transcriptional regulation, DNA replication and repair, RNA editing, and bacterial motility. The requested technology will therefore open up novel possibilities for research on biological pathways relevant to cancer—including the testing of candidate therapeutics—and enable junior faculty to write R01 grant applications that could otherwise not be written. In doing so, the C-Trap will place Einstein at the forefront of innovative, single-molecule cancer research and thereby increase the recognition of Einstein, its faculty, and the Einstein Cancer Center in particular. Just four years ago, the Nobel prize for physics was awarded to Arthur Ashkin for the invention of optical tweezers, highlighting the innovative nature of this technology and the timely manner of this proposal. In making the C- Trap available to cancer biologists at Einstein, new cancer therapies and treatments can be developed, which will benefit human health.

项目概要 该提案的目标是获得最先进的光镊仪器与多色相结合 Gruss- 中最近创建的单分子分析核心 (CSMA) 的单分子成像 具体来说，我们建议阿尔伯特·爱因斯坦医学院（Einstein）的利珀生物光子学中心（GLBP）。 制作 LUMICKS 的 C-Trap（双光镊结合 3 色共焦成像和多通道 爱因斯坦的研究人员可以通过专家培训、咨询和合作来使用微流体技术。 由于光镊与多色荧光显微镜的集成，C-Trap 允许相关 进行力（机械传感）和荧光测量，从而能够进行广泛的研究 一系列生物过程，包括 DNA 和 RNA-蛋白质相互作用的单分子研究 （转录、DNA 和 RNA 复制以及 DNA 修复）、翻译、蛋白酶体降解、相 C-Trap 放置在 CSMA 中将极大地促进分离和免疫学等研究领域的发展。 加强和加速 NIH 资助的爱因斯坦项目，其中许多项目非常适合单分子研究 并将受益于这项技术，特别是 C-Trap 将用于研究 微管运输系统的调节、染色质组织和功能、转录调节、 DNA 复制和修复、RNA 编辑和细菌运动因此将开放。 研究与癌症相关的生物途径的新可能性——包括候选药物的测试 疗法——并使初级教师能够撰写原本无法撰写的 R01 资助申请。 这样做，C-Trap 将使爱因斯坦处于创新、单分子癌症研究的前沿， 从而提高对爱因斯坦、其教员以及爱因斯坦癌症中心的认可度，尤其是四个。 几年前，诺贝尔物理学奖授予阿瑟·阿什金（Arthur Ashkin），以表彰他发明光镊， 强调了这项技术的创新性以及这项提案的及时性。 爱因斯坦的癌症生物学家可以使用陷阱，可以开发新的癌症疗法和治疗方法，这 将有益于人类健康。