Biospecimen Repository Core

生物样本存储库核心

基本信息

批准号：
10453630
负责人：
Hikmat Al-Ahmadie
金额：
$ 19.96万
依托单位：
SLOAN-KETTERING INST CAN RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-24 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10453630
关键词：
Animals Antibodies BCG Live Bacillus Calmette-Guerin Therapy Bioinformatics Biological Biological Assay Biological Markers Biological Specimen Banks Biometry Blood Blood specimen Cell Separation Cisplatin Clinical Clinical Chemistry Clinical Laboratory Improvement Amendments Clinical Research Communities Consent Consultations DNA DNA Damage DNA analysis Data Diagnostic Disease Disease Management Doctor of Philosophy Documentation Enrollment Ensure Evaluation Excision Formalin Freezing Fresh Tissue Gene Expression Profiling Genes Genetic Genomics Goals Guidelines Heterogeneity Human Image Immunohistochemistry In Situ Hybridization Inherited Institutional Review Boards Intervention Label Laboratories Lead Leadership Malignant Neoplasms Malignant neoplasm of ureter Malignant neoplasm of urinary bladder Measures Memorial Sloan-Kettering Cancer Center Methodology Microdissection Modeling Molecular Morphology Mus Mutate Mutation Needle biopsy procedure Neoplasm Metastasis Nucleic Acids Paraffin Embedding Pathologic Pathology Patient-Focused Outcomes Patients Pelvic Cancer Phenotype Pilot Projects Plasma Play Preparation Process Proteomics Protocols documentation Public Health Quality Control RNA Radical Cystectomy Recording of previous events Recurrence Renal carcinoma Research Research Personnel Research Project Grants Resource Sharing Risk Role Sampling Scanning Serum Services Specimen Standardization T cell clonality T cell receptor repertoire sequencing Therapeutic Therapeutic Human Experimentation Tissue Embedding Tissue Microarray Tissue Procurements Tissue Sample Tissues Translational Research Transurethral Resection Tube United States Urethral Cancer Urine Urothelium Whole Blood Work Xenograft Model advanced disease anticancer research base cell free DNA clinical sequencing data quality design digital exome sequencing exosome follow-up genetic analysis human tissue immune checkpoint blockade improved laser capture microdissection molecular diagnostics mouse model neoplasm resource next generation sequencing novel novel marker patient privacy predictive marker prognostic programmed cell death ligand 1 prospective prospective test quality assurance relational database response response biomarker tissue archive transcriptome sequencing tumor

项目摘要

PROJECT SUMMARY/ABSTRACT The Biospecimen Repository Core is designed to provide support to the basic translational research efforts of the SPORE in Bladder Cancer. The Core will play a central role in collecting, annotating, storing, distributing, and tracking urothelial cancer biospecimens (tissue, urine, and blood) from patients enrolled in biospecimen banking and therapeutic research protocols. Detailed biospecimen annotation, including documentation of pre- analytic processing variables, pathology findings, and patient clinical history information will be recorded in robust relational databases. We will conduct rigorous data quality assurance and quality control measures, and standardized longitudinal follow-up of all consented patients with materials in the urothelial cancer biospecimen repository. The Core will also provide SPORE investigators with expert histopathological evaluation of tumor samples both from patients enrolled on research protocols and from xenograft models. The Core will also provide assistance in performing and interpreting immunohistochemical and in situ hybridization assays, in selecting tissue for microdissection and construction of arrays. Given the significant morphologic heterogeneity of bladder cancers, these services require expertise beyond what MSK Pathology Core can provide, underscoring the importance of the role and input of the Core Directors. The specific aims of the core are: Specific Aim 1. To maintain and expand a model urothelial cancer resource designed to collect, annotate, store, process, and distribute biospecimens for translational urothelial cancer research. Specific Aim 2. To perform systematic pathologic evaluation of all human and animal biospecimens and preparation of appropriate biospecimens for use by SPORE investigators. Each of the research projects relies extensively on the Biospecimen Repository Core to achieve their translational research objectives. For RP-1, the core will aid with the prospective molecular characterization of urothelial cancers for mutations in DNA damage response genes and will assist with the preparation of tumor samples for whole-exome sequencing to identify novel biomarkers of cisplatin-sensitivity and with blood and urine samples for cell-free DNA analysis. For RP-2, the core will provide blood samples and matched tumor for next-generation sequencing analyses to identify genes, which when mutated are associated with increased inherited risk for urothelial cancer. For RP-3, the core will provide blood and tissue samples for multi-platform analyses (PD-L1 immunohistochemistry, T-cell clonality, tumor mutational load, whole exome sequencing (WES), RNAseq, TCRseq, immunoSEQ and MDSC quantitation) with the goal of identifying predictive biomarkers of response to immune checkpoint blockade. For RP-4, the Biospecimen Repository Core will assist in the characterization of xenograft models used to study novel strains of bacillus Calmette-Guérin (BCG) and with the analysis of samples from patients with urothelial cancer to identify predictive biomarkers of response to BCG immunotherapy.

项目摘要/摘要 Biospecimen Repository Core旨在为基本的翻译研究工作提供支持膀胱癌的孢子。核心将在收集，注释，存储，分发，并跟踪入学的患者的尿路上皮癌生物测量（组织，尿液和血液）银行和治疗研究方案。详细的生物传感注释，包括分析处理变量，病理发现和患者临床病史信息将记录在强大的关系数据库。我们将采取严格的数据质量保证和质量控制措施，以及所有同意患者在尿路上皮癌生物测试中的标准化纵向随访存储库。核心还将为孢子研究人员提供对肿瘤的专家组织病理学评估来自招募研究方案和异种移植模型的患者的样本。核心也将在执行和解释免疫组织化学和原位杂交分析方面提供帮助选择用于显微解剖和构建阵列的组织。鉴于明显的形态异质性在膀胱癌中，这些服务需要专业知识，而不是MSK病理核心提供的服务，强调核心董事角色和输入的重要性。核心的具体目的是：特定目的1。维护和扩展旨在收集，注释，存储，处理和分发生物测量，以进行翻译的尿路上皮癌研究。具体目的2。对所有人类和动物生物测量进行系统的病理评估以及准备适当的生物测量供孢子研究者使用。每个研究项目都完全依靠生物测量存储库来实现其翻译研究目标。对于RP-1，核心将有助于前瞻性分子表征尿路上皮癌用于DNA损伤反应基因突变，并将有助于制备肿瘤用于全外活体测序的样品，以鉴定顺铂敏感性以及血液和血液和血液的新生物标志物尿液样品进行无细胞DNA分析。对于RP-2，核心将提供血液样本和匹配的肿瘤下一代测序分析以鉴定基因，当突变与增加有关继承了尿路上皮癌的风险。对于RP-3，核心将为多平台提供血液和组织样本分析（PD-L1免疫组织化学，T细胞克隆性，肿瘤突变负荷，整个外显子组测序（WES），RNASEQ，TCRSEQ，IMMUNOSEQ和MDSC定量），目的是确定预测性对免疫检查点封锁的反应的生物标志物。对于RP-4，Biospecimen存储库核心将协助特征的异种移植模型用于研究新型芽孢杆菌Calmette-guérin的菌株（BCG）以及分析来自尿路上皮癌患者的样品，以鉴定对BCG免疫疗法的反应。