Untargeted Analysis Resource

无针对性的分析资源

基本信息

批准号：
10453436
负责人：
Manish Arora
金额：
$ 89.47万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-11 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10453436
关键词：
Age Automatic Data Processing Behavior Biological Biological Assay Biological Markers Cellular Structures Chemicals Child Client Communication Complex Computer software Computing Methodologies Consultations Counseling Data Data Reporting Data Set Database Management Systems Development Developmental Biology Diet Dietary Component Disease Elements Ensure Environment Environmental Exposure Environmental Health Environmental Pollutants Environmental Risk Factor Exposure to Funding Generations Genetic Genome Scan Grant Health Hormones Human Industrialization Information Management Ions Journals Knowledge Label Laboratories Learning Libraries Life Life Cycle Stages Longevity Mass Spectrum Analysis Measures Metabolic Pathway Metadata Methodology Methods Molecular Molecular Profiling Molecular Target Monitor Nature Occupations Ontology Pathogenicity Pharmaceutical Preparations Phenotype Physiology Preparation Procedures Process Productivity Proteomics Protocols documentation Publications Quality Control Reporting Research Research Design Research Personnel Resources Robin bird Sampling Sampling Studies Scanning Science Services Shapes Specimen Systems Biology Testing Time United States National Institutes of Health Urine Work analytical method base cost effectiveness data centers data quality design environmental chemical experience high standard high throughput technology human error instrument laboratory equipment lipidomics meetings metabolomics microbial microbiome microorganism multidimensional data multidisciplinary novel outreach prenatal environmental exposure prevent programs quality assurance response screening social stressor symposium toxic metal ultra high resolution virome wasting

Age Automatic Data Processing Behavior Biological Biological Assay Biological Markers Cellular Structures Chemicals Child Client Communication Complex Computer software Computing Methodologies Consultations Counseling Data Data Reporting Data Set Database Management Systems Development Developmental Biology Diet Dietary Component Disease Elements Ensure Environment Environmental Exposure Environmental Health Environmental Pollutants Environmental Risk Factor Exposure to Funding Generations Genetic Genome Scan Grant Health Hormones Human Industrialization Information Management Ions Journals Knowledge Label Laboratories Learning Libraries Life Life Cycle Stages Longevity Mass Spectrum Analysis Measures Metabolic Pathway Metadata Methodology Methods Molecular Molecular Profiling Molecular Target Monitor Nature Occupations Ontology Pathogenicity Pharmaceutical Preparations Phenotype Physiology Preparation Procedures Process Productivity Proteomics Protocols documentation Publications Quality Control Reporting Research Research Design Research Personnel Resources Robin bird Sampling Sampling Studies Scanning Science Services Shapes Specimen Systems Biology Testing Time United States National Institutes of Health Urine Work analytical method base cost effectiveness data centers data quality design environmental chemical experience high standard high throughput technology human error instrument laboratory equipment lipidomics meetings metabolomics microbial microbiome microorganism multidimensional data multidisciplinary novel outreach prenatal environmental exposure prevent programs quality assurance response screening social stressor symposium toxic metal ultra high resolution virome wasting

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项目摘要

SUMMARY UNTARGETED RESOURCE The Untargeted Analysis Resource is a multi-disciplinary exposomic program designed to integrate environmental measures and biological response into existing NIH studies. We hold weekly Steering Committee meetings of the full Lab Hub team to discuss jobs, troubleshoot workflows, and prioritize resources and activities in order to provide state-of-the-art service to HHEAR clients. Our team has considerable expertise in matching the underlying systems biology of untargeted exposure biomarkers to the study design and health phenotypes, with a range the biological specimen types that would be typically available in health studies. We also factor in subject age, occupation and the likely exposure scenarios. Our lab feasibility assessments help shape the science of a client's submission towards higher impact studies. We also counsel clients on preventing the wasting of precious samples on assays they may request, but which are deemed likely to be of limited values based on our many years of research experience and understanding of exposure scenarios. We work with the Administrative Core to coordinate services, including sample management, receiving, labeling and tracking. We serve as expert consultants with the Coordinating Center, PI/Clients and other HHEAR Resources. We collaborate with the Data Center in the management of results reporting, ensuring that data are properly formatted and have sufficient metadata, as well as participate in the analysis of exposure/phenotype relationships. We use automated sample handling and preparation methods and high throughput analytical procedures and rigorous inter-laboratory QA/QC procedures which include real time analysis monitoring and database management through state-of-the-art laboratory information management software, as well as automated data processing to reduce human error. Our methods ensure proper sample tracking and data reporting and avoid mismatches of samples to results. With substantial institutional resources at its disposal, the Untargeted Resource has doubled the number of mass spectrometry instruments to 14 in the last 3 years, ensuring our capacity to serve the needs of the HHEAR Network. Our team also conducts extensive outreach and was the only CHEAR lab hub to organize a National Exposome Symposium (held Nov 2-3, 2018 at Mount Sinai and themed on Exposomics) as described in the Administrative Core. QA/QC protocols and standard operating procedures for all target compounds have been established to maintain the quality of the chemical analysis. We participated in all CHEAR mandated QA/QC and PT programs including the round-robin analysis of a pooled urine sample between all Untargeted CHEAR Labs, and will do so for HHEAR. Overall, we process and analyze samples with a high degree of efficiency and accuracy, provide expert scientific consultation and integrate our work with the other Hub components while maintaining the highest standards of productivity and cost-effectiveness.

摘要非目标资源未定位的分析资源是一个旨在集成的多学科展开计划环境措施和生物学反应对现有的NIH研究。我们每周转向完整实验室中心团队的委员会会议讨论工作，解决工作流程和资源优先级和活动，以便为Hhear客户提供最先进的服务。我们的团队有很大的与研究设计相匹配的基础系统生物学的专业知识和健康表型，范围是通常可以在健康中获得的生物标本类型研究。我们还考虑主题年龄，职业和可能的暴露情况。我们的实验室可行性评估有助于塑造客户提交高度影响研究的科学。我们也建议客户可能会要求浪费珍贵样品的客户，但被认为是根据我们多年的研究经验和对暴露的理解，可能具有有限的价值方案。我们与行政核心合作以协调服务，包括样本管理，接收，标签和跟踪。我们是协调中心，PI/客户的专家顾问其他HHEAR资源。我们与数据中心合作，管理结果报告的管理确保数据的格式正确并具有足够的元数据，并参与分析暴露/表型关系。我们使用自动样品处理和制备方法以及高吞吐量分析程序和严格的实验室间质量检查/QC程序，包括实时通过最先进的实验室信息管理分析监视和数据库管理软件以及自动数据处理以减少人为错误。我们的方法可确保正确样本跟踪和数据报告，并避免样本不匹配到结果。具有实质性的机构不靶向资源的资源使质谱工具的数量翻了一番在过去的三年中，到14岁，确保我们满足HHEAR网络需求的能力。我们的团队也是如此进行广泛的外展活动，并且是唯一组织全国性展览会研讨会的奇尔实验室中心（2018年11月2日至3日在西奈山举行，主题为Exposomics）如行政核心中所述。已经确定了所有目标化合物的QA/QC协议和标准操作程序保持化学分析的质量。我们参加了所有Chear命令的QA/QC和PT 包括对所有未靶向的Chear Labs之间合并尿液样本的旋转分析的程序，并为HHEAR做到这一点。总体而言，我们以高效率处理和分析样品，准确性，提供专家科学咨询，并将我们的工作与其他枢纽组件集成在一起保持最高的生产率和成本效益。