Agilent 6490 Triple Quadrupole Mass Spectrometer

Agilent 6490 三重四极杆质谱仪

• 批准号: 8640509
• 负责人: JULIE D SABA
• 金额: $ 52.34万
• 依托单位: CHILDREN'S HOSPITAL & RES CTR AT OAKLAND
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640509
• 关键词: Amino Acids; Binding Proteins; Biological Markers; Cardiovascular Diseases; Cells; Clinical; Complex; Core Facility; Coupled; Detection; Diabetes Mellitus; Disease; Drug Delivery Systems; Equipment; Funding; Genetic; Health; High Pressure Liquid Chromatography; Immunology; Inflammation; Institutes; Lipid Binding; Lipids; Lipoproteins; Liquid Chromatography; Longevity; Metabolism; Methods; Nanotechnology; Nutrient; Pediatric Hospitals; Pediatric Research; Performance; Pharmaceutical Preparations; Pharmacologic Substance; Phospholipids; Proteins; Recruitment Activity; Reproducibility; Request for Proposals; Research; Research Institute; Research Personnel; Research Project Grants; Resources; Sampling; Scientist; Sickle Cell Anemia; Sphingolipids; System; Technology; Thalassemia; Time; United States National Institutes of Health; Vitamin D; anticancer research; arm; experience; innovation; instrument; instrumentation; mass spectrometer; metabolomics; millisecond; multiple reaction monitoring; nutrition; obesity prevention; programs; ultra high pressure; vaccine development

DESCRIPTION (provided by applicant): Children's Hospital Oakland Research Institute (CHORI) is a free-standing institute that serves as the research arm of the Children's Hospital & Research Center Oakland. Our research program is among the top pediatric research departments in the nation with regard to funding from the National Institutes of Health (NIH). Research is heavily focused on lipids and lipoproteins in health and disease. This proposal requests funds to purchase an Agilent 6490 Triple Quadrupole mass spectrometer (MS) equipped with 1290 Infinity Quaternary liquid chromatography (LC) system. This LC/triple quadrupole MS combined system would replace an out-of- date LC/MS system that is rapidly approaching the end of its lifespan. LC/MS instrumentation is a critical resource for a large and diverse array of research projects involving metabolomics, analysis of biomarkers of disease and inflammation, drug delivery using nanotechnology, lipid quantitation, and analysis of lipid-binding proteins. The instrument would be used to quantify target molecules including sphingolipids, vitamin D, amino acid metabolites, thiolated and oxidized proteins, phospholipids, oxysterols and metabolites of statin drugs. Twelve major users representing a cross-section of CHORI's major scientific centers, including Cancer Research; Sickle Cell Disease & Thalassemia; Nutrition & Metabolism; Immunology & Vaccine Development; Prevention of Obesity, Cardiovascular Disease & Diabetes; and Genetics will be supported by the instrument. The 6490, with iFunnel Technology, enables zeptomole detection limits at conventional HPLC flow rates. The 6490 instrument provides unsurpassed sensitivity for the analysis of pharmaceutical compounds, trace-level nutrients, clinical samples, drugs, metabolites, and biomarkers. With 1 millisecond dwell times and no collision cell cross talk, the 6490 QqQ is ideally coupled with the 1290 ultra high-pressure/performance LC (UHPLC) system for fast sample analyses, allowing reliable peak integration and quantitation across even 1 second-wide UPHLC peaks. For very complex sample mixtures, the 6490 QqQ allows dynamic multiple reaction monitoring (MRM) methods with up to 4,000 transitions per method and polarity switching in as little as 30 milliseconds. The Agilent 6490 QqQ is a versatile, state-of-the-art instrument with high sensitivity, linearity, dynamic range, reproducibility and robustness and which represents a technology with which our user group is highly familiar. Thus, we have chosen this as the optimal system from a number of competing MS systems. The new LC/MS system will significantly advance the current projects and add to the pool of shared equipment at CHORI. CHORI's scientists are adept at LC/MS; we have long-standing experience in pioneering MS technology and maintaining an active MS Core Facility. We have also recruited external scientific advisors to further insure the instrument is utilized properly and to the highet capacity possible for innovative research by the largest number of CHORI investigators and potentially by external investigators through collaborative research endeavors.

描述（由申请人提供）： 奥克兰儿童医院研究所 (CHORI) 是一个独立机构，是奥克兰儿童医院和研究中心的研究机构。在美国国立卫生研究院 (NIH) 的资助下，我们的研究项目位居全美顶级儿科研究部门之列。研究主要集中在健康和疾病中的脂质和脂蛋白。该提案要求资金购买配备 1290 Infinity 四元液相色谱 (LC) 系统的 Agilent 6490 三重四极杆质谱仪 (MS)。这种 LC/三重四极杆 MS 组合系统将取代过时的 LC/MS 系统，该系统的使用寿命很快即将结束。 LC/MS 仪器是大量多样化研究项目的关键资源，涉及代谢组学、疾病和炎症生物标志物分析、使用纳米技术的药物输送、脂质定量和脂质结合蛋白分析。该仪器将用于量化目标分子，包括鞘脂、维生素 D、氨基酸代谢物、硫醇化和氧化蛋白质、磷脂、氧甾醇和他汀类药物的代谢物。代表 CHORI 主要科学中心（包括癌症研究中心）各个领域的 12 个主要用户；镰状细胞病和地中海贫血；营养与代谢；免疫学与疫苗开发；预防肥胖、心血管疾病和糖尿病；仪器将支持遗传学。 6490 采用 iFunnel 技术，可在传统 HPLC 流速下实现 zeptomole 检测限。 6490 仪器为药物化合物、痕量营养素、临床样品、药物、代谢物和生物标志物的分析提供了无与伦比的灵敏度。 6490 QqQ 的停留时间为 1 毫秒，无碰撞池串扰，可与 1290 超高压/高性能 LC (UHPLC) 系统完美结合，实现快速样品分析，即使在 1 秒宽的 UPHLC 中也能实现可靠的峰积分和定量峰。对于非常复杂的样品混合物，6490 QqQ 允许动态多反应监测 (MRM) 方法，每种方法最多可进行 4,000 个离子对，极性切换仅需 30 毫秒。 Agilent 6490 QqQ 是一款多功能、最先进的仪器，具有高灵敏度、线性度、动态范围、重现性和稳健性，代表了我们的用户组非常熟悉的技术。因此，我们从众多竞争性 MS 系统中选择了该系统作为最佳系统。新的 LC/MS 系统将显着推进当前的项目，并增加 CHORI 的共享设备库。 CHORI 的科学家擅长 LC/MS；我们在开拓 MS 技术和维护活跃的 MS 核心设施方面拥有长期的经验。我们还招募了外部科学顾问，以进一步确保该仪器得到正确使用，并确保尽可能多的 CHORI 研究人员以及外部研究人员通过合作研究努力进行创新研究。

项目成果

Randomized nutrient bar supplementation improves exercise-associated changes in plasma metabolome in adolescents and adult family members at cardiometabolic risk.

随机营养棒补充剂可改善具有心脏代谢风险的青少年和成年家庭成员与运动相关的血浆代谢组变化。

• 发表时间: 2020
• 影响因子: 3.7
• 作者: Mietus;Narayanan, Nisha;Krauss, Ronald M;Laine;McCann, Joyce C;Shigenaga, Mark K;McHugh, Tara H;Ames, Bruce N;Suh, Jung H
• 通讯作者: Suh, Jung H

Efficacy of AAV9-mediated SGPL1 gene transfer in a mouse model of S1P lyase insufficiency syndrome.

AAV9 介导的 SGPL1 基因转移在 S1P 裂解酶不足综合征小鼠模型中的功效。

• 发表时间: 2021
• 影响因子: 8
• 作者: Zhao, Piming;Tassew, Gizachew B;Lee, Joanna Y;Oskouian, Babak;Muñoz, Denise P;Hodgin, Jeffrey B;Watson, Gordon L;Tang, Felicia;Wang, Jen;Luo, Jinghui;Yang, Yingbao;King, Sarah;Krauss, Ronald M;Keller, Nancy;Saba, Julie D
• 通讯作者: Saba, Julie D

Gene therapy with AAV9-SGPL1 in an animal model of lung fibrosis.

在肺纤维化动物模型中使用 AAV9-SGPL1 进行基因治疗。

• DOI: 10.1002/path.6256
• 发表时间: 2024-02-09
• 期刊: The Journal of Pathology
• 作者: Aritra Bhattacharyya;Ranjha Khan;Joanna Y Lee;G. Tassew;Babak Oskouian;M. L. Allende;Richard L. Proia;Xiaoyang Yin;J. G. Ortega;Mallar Bhattacharya;Julie D. Saba
• 通讯作者: Julie D. Saba