Pediatric Biospecimen Procurement Center (BPC) Supporting the Developmental Gene Expression (dGTEx) Project

儿科生物样本采购中心 (BPC) 支持发育基因表达 (dGTEx) 项目

基本信息

批准号：
10311912
负责人：
THOMAS G BLANCHARD
金额：
$ 335万
依托单位：
NATIONAL DISEASE RESEARCH INTERCHANGE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-09 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10311912
关键词：
Address Adult Anatomy Authorization documentation Bioinformatics Brain Brain Pathology Brain imaging Caring Cells Cellular biology Child Childhood Clinical Clinical Investigator Collection Communities Data Data Analyses Data Collection Data Storage and Retrieval Development Developmental Gene Diagnosis Disease Evaluation Family Gene Expression Genetic Variation Genotype Genotype-Tissue Expression Project Grant Hospitals Human body Informatics Infrastructure Investigation Laboratories Lead Maryland Melissa Methodology Methods Modeling Molecular Molecular Profiling Organ Procurements Pathologic Pathologist Pathology Pediatric Hospitals Pediatric Research Pediatric cohort Philadelphia Physiology Population Principal Investigator Procedures Process Quality Control Recovery Reproducibility of Results Research Research Design Research Personnel Resources Risk Sampling Scientist Ships Site Specimen Structure Technical Expertise Techniques Tissue Banks Tissue Donations Tissue Sample Tissue imaging Tissues Training United States National Institutes of Health Universities biobank body system brain research data exchange design diversity and equity early childhood ethical legal social implication experimental analysis health disparity health equity improved insight medical specialties novel operation pathology imaging prepuberty programs quality assurance racial and ethnic research and development single cell analysis social culture success whole slide imaging

项目摘要

Program Director/Principal Investigator (Bell, Thomas, J.): Project Summary: Accelerating scientific discoveries across the entire spectrum of pediatric research requires advancing our current understanding of the unique molecular profiles and physiologies in all healthy tissues across the human body. The developmental Genotype-Tissue Expression (dGTEx) project will establish the first comprehensive public resource correlating gene expression and genetic variation in pediatric tissues from all major organ systems in the human body. This project will identify the molecular profiles in ~30 distinct tissue types from four developmental groups: Early post-natal, Early childhood, Pre-pubertal, and Post-pubertal. dGTEx will capitalize on new emerging methodologies to capture detailed evaluations on the developing brain and include options for single cell analysis on multiple tissues. To advance our understanding of the Ethical, Legal, Social Implications (ELSI) in pediatric tissue donation, dGTEx also includes an ELSI study to analyze these factors. The data from dGTEx will provide an unrivaled research resource for clinicians and scientists to gain new insights on the molecular milestones of regulatory processes that direct the development of healthy tissues. To support the advancement of dGTEx, this proposal will establish the pediatric Biospecimen Procurement Center (BPC) to provide the pediatric Laboratory Data Analysis Collection Center (LDACC) with suitable tissue samples for their analysis. Our proposal includes a multi-institutional effort with extensive expertise in pediatric recoveries, research, pathology, imaging, biobanking, brain research, and cutting-edge molecular techniques, such as single cell analysis. The objectives for our BPC recovery team will be led by a collaborative effort amongst: 1) National Disease Research Interchange (NDRI) to provide project management and coordinate the recovery collection effort from a network of TSS, 2) Children's Hospital of Philadelphia (CHOP) to process, store, and coordinate pathological review of all non-brain tissues and manage the bioinformatics workflow, and 3) NeuroBiobank at University of Maryland (NBB-Maryland) to process, store, and provide pathological review of brains. This breadth of expertise is essential for the BPC to provide well-annotated, suitable biospecimens to enable reliable and reproducible results from the LDACC's cutting-edge experimental methods and rigorous data interpretations. Improving our understanding of ELSI-specific challenges in pediatric tissue donation is an unmet need for the research community. To enable a more complete analysis of these challenges, Johns Hopkins All Children's Hospital (JHACH) will coordinate the dGTEx ELSI study to include evaluations of Tissue Requesters (TRs) and Family Decision Makers (FDMs) of Deceased and At-Risk Children. Collectively, our BPC team is committed to developing an all-encompassing, unparalleled BPC for the dGTEx project to empower the advancement of pediatric research, ELSI challenges, and the development of new clinical approaches to treat pediatric disorders. OMB No. 0925-0001/0002 (Rev. 03/2020 Approved Through 02/28/2023) Page Continuation Format Page

计划主任/首席研究员（Bell，Thomas，J。）：项目摘要：在整个小儿研究中加速科学发现需要提高我们的当前对人类所有健康组织中所有健康组织中独特的分子谱和生理的了解身体。发展基因型 - 组织表达（DGTEX）项目将建立第一个全面的公共资源将所有主要器官的小儿组织的基因表达和遗传变异相关联人体中的系统。该项目将在〜30种不同的组织类型中识别分子曲线。发展群体：早期产后，幼儿期，素前和素后。 DGTEX将大写关于新的新兴方法，以捕获有关发展中大脑的详细评估，并包括多个组织的单细胞分析。促进我们对道德，法律，社会影响的理解（ELSI）在小儿组织捐赠中，DGTEX还包括一项ELSI研究，以分析这些因素。 DGTEX的数据将为临床医生和科学家提供无与伦比的研究资源，以获得新的见解关于指导健康组织发展的调节过程的分子里程碑。支持 DGTEX的进步，该提案将建立小儿生物遇到采购中心（BPC）为小儿实验室数据分析收集中心（LDACC）提供合适的组织样品分析。我们的建议包括一项多机构的努力，在儿科恢复方面具有广泛的专业知识，研究，病理，成像，生物群，大脑研究和尖端分子技术，例如单个细胞分析。我们BPC恢复团队的目标将由以下合作努力领导：1）国家疾病研究互换（NDRI）提供项目管理并协调恢复收集 TSS网络的努力，2）费城儿童医院（CHOP）进行处理，存储和协调所有非脑组织的病理评论并管理生物信息学工作流程，以及3）神经ob。马里兰州大学（NBB-Maryland）处理，存储和提供对大脑的病理评论。这个广度专业知识对于BPC提供良好的，合适的生物测量以启用可靠和 LDACC的尖端实验方法和严格的数据解释可再现的结果。提高我们对小儿组织捐赠中ELSI特定挑战的理解是对研究社区。为了对这些挑战进行更完整的分析，约翰·霍普金斯（Johns Hopkins）所有儿童医院（JHACH）将协调DGTEX ELSI研究，包括对组织请求者（TRS）和死者和高危儿童的家庭决策者（FDMS）。总的来说，我们的BPC团队承诺为DGTEX项目开发一个无与伦比的无与伦比的BPC，以增强儿科研究，ELSI挑战以及新的临床方法治疗小儿疾病的发展。 OMB No. 0925-0001/0002（Rev. 03/2020通过02/28/2023批准）页面延续格式页面