Detecting RORgt Citrullination

检测 RORgt 瓜氨酸化

基本信息

批准号：
10304200
负责人：
I-CHENG HO
金额：
$ 8.56万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-11-17 至 2022-10-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10304200
关键词：
Address Affect Amino Acids Antibodies Arginine Attenuated Autoimmune Diseases Autoimmunity Cell physiology Cells Citrulline Clinical DNA Binding Detection Future GATA3 gene Generations Genetic Transcription Genomics Goals Health Human Immune response Infection Inflammation Invaded Knowledge Lymphocyte Lymphocyte Function Mammals Mediating Modification Monoclonal Antibodies Names Pathogenicity Peptides Play Post-Translational Protein Processing Process Protein-arginine deiminase Proteins Reagent Regulation Regulatory T-Lymphocyte Role Site Site-Directed Mutagenesis Th2 Cells Untranslated RNA Western Blotting design experimental study human disease immune function improved neutrophil new therapeutic target novel strategies polyclonal antibody protein function tool transcription factor

Address Affect Amino Acids Antibodies Arginine Attenuated Autoimmune Diseases Autoimmunity Cell physiology Cells Citrulline Clinical DNA Binding Detection Future GATA3 gene Generations Genetic Transcription Genomics Goals Health Human Immune response Infection Inflammation Invaded Knowledge Lymphocyte Lymphocyte Function Mammals Mediating Modification Monoclonal Antibodies Names Pathogenicity Peptides Play Post-Translational Protein Processing Process Protein-arginine deiminase Proteins Reagent Regulation Regulatory T-Lymphocyte Role Site Site-Directed Mutagenesis Th2 Cells Untranslated RNA Western Blotting design experimental study human disease immune function improved neutrophil new therapeutic target novel strategies polyclonal antibody protein function tool transcription factor

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项目摘要

Project Summary Many proteins are modified after they are produced in cells. Such modification can critically influence the function of proteins. One such modification is citrullination, which has been shown to regulate the function of immune cells, including lymphocytes and neutrophils. Abnormal citrullination has been associated with many human diseases, including infection and autoimmune diseases. Thus, manipulating protein citrullination can be beneficial in many clinical settings; however, our knowledge of citrullination is still very limited mainly due to lack of sensitive and specific tool for detecting citrullination. The objectives of this project are to develop a reagent that can reliably detect the citrullination of a protein named RORgt, which is important for the differentiation/function of lymphocytes, and to demonstrate the value of this reagent in a proof-of-principle experiment. The reagent thus generated will be a powerful tool for future studies on the regulatory roles of citrullination in the function of lymphocytes and very likely will lead to discoveries of novel therapeutic targets of human diseases.

项目摘要在细胞中产生许多蛋白质后，它们会经过修饰。这种修改会严重影响蛋白质的功能。一种这样的修改是柠檬化，已证明可以调节免疫细胞，包括淋巴细胞和中性粒细胞。瓜氨酸异常与许多人类疾病，包括感染和自身免疫性疾病。因此，操纵蛋白柠檬酸可以是在许多临床环境中有益；但是，我们对柠檬化的了解仍然非常有限，主要是由于缺乏敏感的特定工具来检测柠檬酸。该项目的目标是开发可以可靠地检测出一种名为rorgt的蛋白质的柠檬酸的试剂，这对淋巴细胞的分化/功能，并证明该试剂在原则上的值实验。因此，生成的试剂将是对未来研究调节作用的强大工具淋巴细胞功能的柠檬化，很可能会导致新的治疗靶标的发现人类疾病。