High Speed Single Pixel Hyperspectral Spatial Frequency Domain Imaging

高速单像素高光谱空间频域成像

• 批准号: 8954906
• 负责人: Randy A. Bartels
• 金额: $ 21.05万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8954906
• 关键词: Algorithms; Animal Model; Biological; Biopsy Specimen; Clinical; Complex; Computer Simulation; Custom; Data; Dimensions; Disease; Exhibits; Fluorescence; Frequencies; Functional Imaging; Goals; Health; Image; Imaging problem; Imaging technology; Lead; Length; Light; Lighting; Maps; Measures; Medical Imaging; Methods; Modeling; Monitor; Operative Surgical Procedures; Optics; Patients; Pattern; Performance; Phase; Printing; Process; Property; Real-Time Systems; Resolution; Sampling; Scanning; Source; Specimen; Speed; Structure; System; Technology; Testing; Time; Tissue Engineering; Tissues; Update; Variant; absorption; attenuation; base; cost; data acquisition; detector; image processing; image reconstruction; imaging modality; imaging platform; imaging system; innovative technologies; instrument; molecular imaging; optical spectra; pre-clinical; quantitative imaging; response; spectrograph; technology development; tissue phantom; tomography

 DESCRIPTION (provided by applicant): Spatial Frequency Domain Imaging (SFDI) is a model-based, wide-field, non-contact method for measuring the absorption, scattering, and fluorescence properties of biological tissue. Optical properties are determined in each pixel simultaneously, by measuring the attenuation (or fluorescence) of sinusoidal patterns of light projected onto the sample at varying spatial frequencies and phases. The mean interrogation depth at a given wavelength is controlled by the spatial frequency of projection, and frequency-dependent differences in path length are used to calculate tissue optical properties using computational models. Images processed to extract SFDI images are recorded on a conventional camera system, which are costly and limited in data acquisition speed. The tissue properties recovered with SFDI vary with optical wavelength. Conventional camera systems are not able to resolve optical wavelengths, so SFDI imaging of the spectral variation in tissue properties would require switching illumination wavelengths and sequentially acquiring SFDI data. Alternatively, complex and expensive hyperspectral cameras can be used, but the cost is prohibitive for general clinical use. To overcome the limitations on SFDI image speed and to enable high speed hyperspectral SFDI imaging, we will develop the capability of SPatIal Frequency modulation for Imaging (SPIFI) to be used for SFDI image extraction with a broad optical spectrum. SPIFI exploits light modulated in space and time to encode spatial information into modulation frequency to that after illuminating an object, light collected on a single pixel detector can be decoded to form images. Single pixel detectors exhibit bandwidths many orders of magnitude larger than camera systems. The large detector bandwidth opens the capability for recording data for spatial and spectral information with a single photodetector that can be processed into hyperspectral images. The strategy of merging SPIFI, SFDI, and hyperspectral imaging will open up capabilities for unprecedented imaging rates of quantitative spectroscopic tissue imaging. In addition, modulation formats applied to the illumination light are adaptable - making single pixel hyperspectral SFDI an extremely agile imaging platform that can adapt the imaging, speed, resolution, and spectral resolution dynamically to optimize imaging conditions for specific target applications. The ability to adaptively optimize imaging of absorption, scattering, or florescence in tissues and build up depth-resolved hyperspectral images will open new capabilities for tissue imaging.

 描述（由申请人提供）： 空间频域成像（SFDI）是一种基于模型的宽视场非接触方法，用于同时确定每个像素的吸收、散射和荧光特性。 ，通过测量以不同的空间频率和相位投射到样品上的光的正弦图案的衰减（或荧光），给定波长下的平均询问深度由投射的空间频率控制，并且路径长度的频率依赖性差异用于使用计算模型来计算组织光学特性，这些图像被处理以提取 SFDI 图像，并记录在传统的相机系统上，该系统成本高昂且数据采集速度有限。传统的相机系统无法解析光学波长，因此对组织特性的光谱变化进行 SFDI 成像需要切换照明波长并依次采集 SFDI 数据，或者可以使用复杂且昂贵的高光谱相机。一般禁止为了克服 SFDI 图像速度的限制并实现高速高光谱 SFDI 成像，我们将开发用于成像的空间调频 (SPIFI) 功能，用于利用宽光谱进行 SFDI 图像提取。在空间和时间上进行调制，将空间信息编码为调制频率，在照射物体后，单像素探测器上收集的光可以被解码以形成图像。单像素探测器表现出许多带宽。比相机系统大几个数量级的探测器带宽使得能够使用单个光电探测器记录空间和光谱信息数据，并将其处理成高光谱图像。融合 SPIFI、SFDI 和高光谱成像的策略将开启多种功能。实现前所未有的定量光谱组织成像成像速率 此外，应用于照明光的调制格式具有适应性 - 使单像素高光谱 SFDI 成为一个极其灵活的成像平台，可以适应成像、速度、分辨率、动态调整光谱分辨率以优化特定目标应用的成像条件，自适应优化组织中的吸收、散射或荧光成像并建立深度分辨高光谱图像的能力将为组织成像开辟新的功能。