Employing smart underwear to measure gut microbial hydrogen sulfide production

利用智能内衣测量肠道微生物硫化氢的产生量

• 批准号: 10425072
• 负责人: Reza Ghodssi
• 金额: $ 22.82万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-10 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10425072
• 关键词: Address; Affect; Anogenital region; Biological Availability; Calories; Clinical Trials; Cohort Studies; Collection; Colorectal Cancer; Complex; Coupled; Cysteine; Devices; Diet; Dietary Factors; Dietary Intervention; Disease; Enzymes; Etiology; Filtration; Flatulence; Future; Gases; Goals; Health; Human; Hydrogen; Hydrogen Sulfide; In Vitro; Intake; Measurement; Measures; Metabolism; Methane; Methods; Miniaturization; Noise; Nutrient; Outcome; Participant; Phase; Physiological; Physiology; Play; Production; Randomized; Research; Role; Signal Transduction; Signaling Molecule; Source; System; Techniques; Testing; Time; Ulcerative Colitis; Validation; arm; comparison intervention; design; dietary; experience; feeding; flexibility; gut microbiota; human microbiota; metal oxide; microbial; microbiome; miniaturized device; precision nutrition; prototype; rectal; sensor; software development; temporal measurement; trimethylamine; wearable device

PROJECT SUMMARY/ABSTRACT The circulating gasotransmitter H₂S is endogenously produced by tightly-regulated human enzymes and exogenously produced in a diet-dependent manner by the human microbiota. Exogenous H2S production by the human gut microbiota influences human health by modulating systemic H2S bioavailability. Excessive gut microbial H2S production is hypothesized to be involved in the etiology of colorectal cancer and Ulcerative Colitis. Our understanding of how gut microbially produced H₂S affects human health remains incomplete because current measurement techniques are imprecise, invasive, require biospecimen collection, and have limited temporal resolution. In this proposal, our objective is to address this gap by developing a research- grade wearable Smart Underwear device that enables long-term real time measurements of gut microbial H₂S production in gaseous rectal effluent (flatus). Flatus are an untapped source for measuring gut microbial H2S production. We have demonstrated that the Smart Underwear v1 prototype can precisely measure physiologically-relevant concentrations of H2S in vitro. When worn by humans, the Smart Underwear v1 prototype detects flatus with an excellent signal-to-noise ratio and is able to estimate the concentration of H2S in each flatus. In the R21 phase, we propose to develop a second revision Smart Underwear prototype for clinical trials through miniaturization, software development, and incorporation of flexible materials. In addition, we propose to conduct a user experience trial to gauge the user acceptance and test the reliability of the Smart Underwear device when worn by humans. In the R33 phase, we will use the Smart Underwear v2 prototype developed in the R21 phase to test the hypothesis that the Smart Underwear device can detect diet-induced alterations in gut microbial H2S production using a randomized controlled feeding trial that contrasts gut microbial H₂S production by modulating dietary cysteine intake. The completion of the proposed goals will lead to the first wearable device that enables long-term real time measurements of gut microbial H2S production. This will facilitate the design of personalized dietary interventions to rationally modulate gut microbial H₂S production and benefit future studies seeking to untangle the complex interactions between gut microbial H2S production and disease.

项目摘要/摘要 循环固定器H₂S由紧密调节的人酶和 人类微生物群以饮食依赖性方式外源产生。外源H2S生产 人类肠道菌群通过调节系统性H2S生物利用度来影响人类健康。肠道过多 假设微生物H2S的产生与结直肠癌和溃疡性的病因有关 结肠炎。我们对肠道微生物如何影响人类健康的理解仍然不完整 因为当前的测量技术是可以稳定的，侵入性的，需要收集生物测量，并且具有 有限的临时分辨率。在这项建议中，我们的目标是通过开发研究来解决这一差距 - 等级可穿戴的智能内衣设备，可实现肠道微生物的长期实时测量 气态直肠效应（FLTUS）的产生。紫菜是测量肠道微生物H2S的未开发的来源 生产。我们已经证明，智能内衣V1原型可以精确测量 体外H2S的生理含量浓度与生理相关的浓度。当人类穿着时，聪明的内衣v1 原型检测具有出色的信号噪声比的肠胃 在每个肠子里。在R21阶段，我们建议开发第二个修订版智能内衣原型 通过微型化，软件开发和柔性材料结合的临床试验。此外， 我们建议进行用户体验试验，以评估用户接受并测试SMART的可靠性 人类佩戴时的内衣装置。在R33阶段，我们将使用智能内衣V2原型 在R21阶段开发，以检验智能内衣装置可以检测饮食引起的假设 肠道微生物H2S生产的改变，使用随机对照喂养试验与肠道形成鲜明对比 微生物通过调节饮食中半胱氨酸的摄入来生产。拟议目标的完成将领导 到第一个可穿戴设备，可实现肠道微生物H2S生产的长期实时测量。 这将有助于设计个性化的饮食干预措施，以合理调节肠道微生物h₂s 生产和利益未来的研究旨在解开肠道微生物H2S之间的复杂相互作用 生产和疾病。