Shared Resource: Biological Imaging Facility

共享资源：生物成像设施

基本信息

批准号：
10427531
负责人：
Timothy Yen
金额：
$ 2.65万
依托单位：
RESEARCH INST OF FOX CHASE CAN CTR
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-07-01 至 2022-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10427531
关键词：
3-Dimensional Abbreviations Adopted Advisory Committees Animals Area Basic Science Bioinformatics Biological Biological Assay Biological Models Biological Specimen Banks Biometry Blood Cells Calendar Cancer Biology Cancer Center Cancer Center Support Grant Cancer Control Caring Cathepsins Cell Culture Techniques Cell Separation Cells Charge Clinical Collaborations Complex Comprehensive Cancer Center Computing Methodologies Coupled Data Data Analyses Development Doctor of Philosophy Drosophila genus Elastases Electron Microscope Ensure Epigenetic Process Equipment Experimental Designs Experimental Models Familiarity Fee-for-Service Plans Fluorescence Fox Chase Cancer Center Foxes Funding Genomics Goals Health system Histopathology Facility Hour Human Resources Image Imaging technology Individual Interleukin-6 Laboratories Laboratory Animals Leadership Light Microscope Luciferases Magnetic Resonance Imaging Malignant Neoplasms Mammalian Cell Metalloproteases Modernization Molecular Molecular Analysis NCI Center for Cancer Research Optics Organ Parents Peer Review Philadelphia Play Population Study Procedures Quality Control Recommendation Research Research Personnel Resolution Resource Sharing Resources Role Sampling Science Services Site Spectral Karyotyping Standardization Structure Students System Technology Therapeutic Time Tissues Training Transgenic Mouse Facility Universities University Hospitals Zebrafish anatomic imaging animal facility animal imaging anticancer research base cancer prevention cost cost effective cost effectiveness experience experimental study high standard high throughput screening imaging facilities imaging system in vivo imaging system instrument medical schools member molecular modeling new technology novel strategies operation peer support programs recruit tomography

项目摘要

CELLULAR/MOLECULAR ANALYSES SHARED RESOURCE: BIOLOGICAL IMAGING FACILITY (BIF) PROJECT SUMMARY/ABSTRACT The Biological Imaging Facility (BIF) provides Cancer Center members the capabilities to obtain the highest quality images to facilitate their research. The BIF under the direction of Timothy Yen, PhD (CB), and a staff of 2.4 FTEs, provide services at the ultrastructural (Electron Microscope (EM)), subcellular and cellular (Light Microscope (LM)) levels, and tissues and organs in small animals (Small Animal Imaging (SAI)). The BIF accommodates diverse experimental models that include mice, zebrafish, Drosophila, and mammalian cells in culture. The BIF staff provides training to use equipment so that users become proficient at collecting and analyzing their own data. Many of the advanced technologies enable real-time studies in live cells and animals. The SAI Manager trains users to operate our high-field MRI system (7-Tesla Bruker DRX) for anatomic imaging, a Caliperls IVIS spectrum that detects luciferase or fluorescently tagged cells in animals, and a Visen FMT2500 which detects the three dimensional distribution of enzymatically activated optical probes (metalloproteinases, cathepsin, elastase) or fluorescently tagged probes in live animals. The BIF offers fee-for- service in cases where investigators cannot devote lab personnel or, for projects requiring EM and SKY (spectral karyotyping) where they rely on the expertise of dedicated staff. The BIF staff is familiar with ongoing projects as they contribute to experimental design and data analysis, the latter of which may require advanced computational methods (i.e. deconvolution, 3D rendering, quantitation, 3D spatial registration of anatomical images obtained from the same animal but using different platforms). Imaging technology plays an essential role in modern cancer research, and is used in a diverse array of laboratories as evidenced by the 30 Cancer Center members who used the BIF in calendar year 2014. 88% of service hours logged for instrument use in 2014 were in support of peer-review funded research. 56 Cancer Center members from all 5 Research Programs used the BIF in the period 2011-2014. The experience of the Facility Director, Yen, is key in implementing state-of-the-art technology and new approaches. Yen's leadership coupled with institutional commitment of $449,600 led to the purchase of two newly acquired Leica SP8 confocal systems. A major value added of the BIF is that it is the most cost effective way to provide users with advanced imaging technologies that are too costly to purchase and support by any individual lab. These services cannot be outsourced. The staff provides stability by ensuring users access to properly functioning instruments. This is critical for time sensitive experiments that involve animals. The BIF is advised by a dedicated Facility Advisory Committee (FAC) that meets annually. Recommendations from the FAC are directed to a governing Facility Parent Oversight Committee that serves to ensure that the BIF continues to have the necessary resources to provide the highest quality imaging services for Cancer Center members. Demand for services is expected to increase in the next cycle of the CCSG due to plans to recruit in all 5 Programs and increased TU usage.

细胞/分子分析共享资源：生物成像设施 (BIF) 项目概要/摘要生物成像设施 (BIF) 为癌症中心成员提供了获得最高水平的能力高质量的图像以促进他们的研究。 BIF 在 Timothy Yen 博士 (CB) 和工作人员的指导下 2.4 FTE，提供超微结构（电子显微镜 (EM)）、亚细胞和细胞（光显微镜（LM））水平以及小动物的组织和器官（小动物成像（SAI））。 BIF 可容纳多种实验模型，包括小鼠、斑马鱼、果蝇和哺乳动物细胞文化。 BIF工作人员提供设备使用培训，使用户能够熟练收集和使用分析自己的数据。许多先进技术可以对活细胞和动物进行实时研究。 SAI Manager 培训用户操作我们的高场 MRI 系统 (7-Tesla Bruker DRX) 进行解剖学检查成像、检测动物荧光素酶或荧光标记细胞的 Caliperls IVIS 光谱以及 Visen FMT2500 可检测酶激活光学探针的三维分布（金属蛋白酶、组织蛋白酶、弹性蛋白酶）或活体动物中的荧光标记探针。 BIF 提供收费服务—— 在调查人员无法投入实验室人员的情况下或需要 EM 和 SKY 的项目时提供服务（光谱核型分析），他们依靠专门工作人员的专业知识。 BIF 工作人员熟悉正在进行的项目，因为它们有助于实验设计和数据分析，后者可能需要先进的计算方法（即反卷积、3D 渲染、定量、解剖学的 3D 空间配准）从同一动物但使用不同平台获得的图像）。成像技术至关重要在现代癌症研究中发挥着重要作用，并被广泛应用于各种实验室，30 种癌症就证明了这一点 2014 日历年使用过 BIF 的中心成员。2014 年记录了 88% 的服务时间用于仪器使用 2014 年支持同行评审资助的研究。来自所有 5 个研究中心的 56 名癌症中心成员 2011-2014 年期间的计划使用了 BIF。设施总监 Yen 的经验对于实施最先进的技术和新方法。日元的领导加上制度承诺购买两台新收购的 Leica SP8 共焦系统，承诺金额为 449,600 美元。一个专业 BIF 的附加值在于它是为用户提供先进成像的最具成本效益的方式任何单个实验室都无法购买和支持的技术成本太高。这些服务不能外包。工作人员通过确保用户使用正常运行的仪器来提供稳定性。这是对于涉及动物的时间敏感实验至关重要。 BIF 由专门的设施咨询机构提供建议委员会 (FAC) 每年举行一次会议。 FAC 的建议将提交至管理机构家长监督委员会旨在确保 BIF 继续拥有必要的资源为癌症中心会员提供最优质的影像服务。服务需求预计由于计划在所有 5 个计划中招募人员以及 TU 使用量的增加，CCSG 的下一个周期有所增加。