Core C: Customized Physiology and Imaging Core

核心C：定制生理学和影像核心

• 批准号: 10230992
• 负责人: Grant Hennig
• 金额: $ 27.19万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-06 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10230992
• 关键词: 3-Dimensional; 3D Print; 4D Imaging; Active Learning; Address; Amyloid beta-Protein; Animals; Basic Science; Blood capillaries; Calcium; Cells; Centers of Research Excellence; Clinical; Complex; Computer software; Confocal Microscopy; Coupled; Custom; Development; Dimensions; Doctor of Philosophy; Electrophysiology (science); Environment; Equipment; Faculty; Faculty Recruitment; Fee-for-Service Plans; Future; Goals; Grant; Human Resources; Hyperemia; Hypertension; Image; Image Analysis; Individual; Infrastructure; Investigation; Laboratories; Learning; Microscope; Microscopy; Modeling; Neurosciences; Nevada; Nomads; Optics; Pericytes; Pharmacology; Physiology; Research; Research Personnel; Resolution; Running; Sampling; Signal Transduction; System; Techniques; Technology; Testing; Three-Dimensional Image; Time; Training; Universities; Vermont; Work; arteriole; awake; base; brain health; cardiovascular health; design; educational atmosphere; experimental study; flexibility; graduate student; heart function; imaging capabilities; imaging modality; imaging system; in vivo imaging; instrument; instrumentation; investigator training; millisecond; mouse model; multi-photon; neuron loss; neurovascular; operation; professor; programs; recruit; replicator; restoration; skills; temporal measurement; tissue/cell preparation

PROJECT SUMMARY The new Customized Imaging and Physiology Core of the proposed Vermont Center for Cardiovascular and Brain Health COBRE will be based upon transformation of a Neuroscience Imaging Core from a previous COBRE (completed in 2016). It will meet the new needs of cutting-edge imaging investigation that will be pursued by Project Directors (PDs) in the newly proposed COBRE. We will employ acquisition of needed technology and renovation to accommodate its operation. The Customized Physiology and Imaging Core will be designed to provide state-of-the-art imaging capabilities for live samples, including intravital, tissue and cell preparations, as well as fixed samples. The new Cardiovascular and Brain Health COBRE will involve extensive training in in vivo imaging and electrophysiology, and exploitation of genetically-encoded indicators and optically-actuated probes by Project Directors. This Core will have high level pre- and post-experiment technical and analytical support from Todd Clason, Dr. Grant Hennig and Dr. Tom Heppner. The new era of high-resolution microscopy requires the flexibility to image in awake animals that may be undergoing specific tasks, and to image in 3 dimensions over a wide range of time periods from milliseconds to weeks. To accomplish this, this Core will advise and train investigators in the use of different types of state-of-art high spatial and temporal resolution microscopy, electrophysiological techniques, and 3D printing for instrument design and fabrication to respond to changing research needs, as well as in use and development of image and electrophysiological analysis software. The goal of this Core is to equip the investigators with imaging/electrophysiological, fabrication and analysis skills so that they are capable of adapting approaches to solve complex problems. It is anticipated that this Core will greatly enhance the competitiveness of COBRE Project Directors as well as campus-wide investigators in cardiovascular and brain health research. This Core is unique in that PDs and other investigators, with support from highly accomplished Core personnel, will acquire the skills to perform the experiments, modify imaging modalities, to fabricate project-specific equipment, and to perform high level image analysis.

项目概要 拟议的佛蒙特州心血管和疾病中心的新定制成像和生理学核心 脑健康 COBRE 将基于神经科学成像核心从以前的 COBRE（2016 年完成）。它将满足尖端成像研究的新需求 由新提议的 COBRE 中的项目总监 (PD) 所追求。我们将采用收购所需的 技术和改造以适应其运营。定制的生理学和成像核心将 旨在为活体样本（包括活体样本、组织和细胞）提供最先进的成像能力 制剂以及固定样品。新的心血管和脑健康 COBRE 将涉及 体内成像和电生理学以及基因编码指标的开发方面的广泛培训 以及项目总监的光学驱动探头。该核心将具有高水平的实验前和实验后 Todd Clason、Grant Hennig 博士和 Tom Heppner 博士提供技术和分析支持。新时代的 高分辨率显微镜需要能够灵活地对可能正在经历特定情况的清醒动物进行成像 任务，并在从几毫秒到几周的广泛时间段内进行 3 维成像。到 为了实现这一目标，该核心将为调查人员提供使用不同类型的最先进的高科技的建议和培训 空间和时间分辨率显微镜、电生理技术和仪器 3D 打印 设计和制造以响应不断变化的研究需求以及图像的使用和开发 和电生理分析软件。该核心的目标是为研究人员配备 成像/电生理学、制造和分析技能，使他们能够适应方法 解决复杂的问题。预计该Core将极大提升COBRE的竞争力 心血管和大脑健康研究的项目主管以及全校研究人员。这个核心 其独特之处在于，PD 和其他调查员在卓有成就的核心人员的支持下，将 获得进行实验、修改成像模式、制造特定项目的技能 设备，并执行高级图像分析。