DBPs

舒张压

• 批准号: 10401764
• 负责人: MICHAEL P ROUT
• 金额: $ 31.03万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10401764
• 关键词: Address; Automobile Driving; Back; Biological; Cells; Chromatin; Communities; Complex; Cytoplasm; DNA; Development; Ensure; Funding; Generations; Genetic Transcription; Methodology; Methods; Microscope; Modeling; Molecular; Morphology; Organelles; Paper; Pathway interactions; Preparation; Process; Proteins; RNA; Reagent; Research; Research Personnel; Sampling; Signal Transduction; Structure; Techniques; Technology; Testing; Time; Training; Translations; feeding; meetings; member; method development; model development; stoichiometry; technology development; tool

PROJECT SUMMARY Driving Biomedical Projects Portfolio To drive the development of our molecular microscope pipeline, we have selected Driving Biomedical Projects (DBPs) that serve as the first stage in applying and testing NCDIR technologies. Each DBP contains one or more key specific technological roadblocks that have been generally acknowledged by the field and that must be overcome by the development and optimization of technologies at different stages in our pipeline. Collectively, these DBPs are selected to test all the technology in this pipeline: helping develop sample preparation methods and ways to isolate intact native assemblies; ensuring the development and refinement of methods that determine the composition, stoichiometry, morphology and connectivity of isolated complexes; and projects that challenge the development of methods for modeling static structures of large macromolecular machines as well as dynamic processes involving these machines on long time scales. We have also selected these DBPs to represent different biological problems, key pieces of the “cellular information pathway” that starts with signalling to a cell, leading to reorganization and activation of DNA in chromatin for transcription into RNA, which is processed, packaged, and exported into the cytoplasm where its translation into protein leads to the generation of new assemblies, structures, and organelles. These DBPs are therefore a key metric for the efficacy of our technologies in both solving key technical roadblocks and addressing key biomedical challenges in the research community we serve. In doing so, we will generate new tools, techniques, and reagents for interactomic studies, train DBP members in the molecular microscope pipeline approaches, begin the dissemination of these approaches to a wider group of researchers both through this training and presentation in meetings and papers, and aid the stabilization of these approaches for a sustainable presence in the community. We also expect application of our TR&D technology in the DBPs to generate new challenges, feeding back to help drive new aspects of our ongoing TR&D development and maturation.

项目摘要 驾驶生物医学项目投资组合 为了驱动分子显微镜管道的开发，我们选择了驱动生物医学项目 （DBP）是应用和测试NCDIR技术的第一阶段。每个DBP包含一个或多个 该领域普遍承认的主要特定技术障碍，这一定是 通过在管道中不同阶段的技术开发和优化克服。共同 选择这些DBP来测试该管道中的所有技术：帮助开发样本准备方法 以及隔离完整的本机组件的方法；确保方法的发展和完善 确定孤立的复合物的组成，化学计量，形态和连通性；和那个 还挑战开发大型大分子机器的静态结构的方法 随着动态过程涉及这些机器的长时间尺度。我们还选择了这些DBP 表示不同的生物学问题，这是从信号开始的“蜂窝信息途径”的关键部分 到一个细胞，导致染色质中DNA的重组和激活以转录为RNA，这是 加工，包装和导出到细胞质中，其转化为蛋白质导致生成 新的组件，结构和细胞器。因此，这些DBP是我们的效率的关键指标 解决关键技术障碍和解决研究中的关键生物医学挑战的技术 我们服务的社区。为此，我们将生成新的工具，技术和试剂，以进行相互作用研究， 在分子显微镜管道方法中训练DBP成员，开始传播这些 通过此培训和论文中的培训和演讲，与更广泛的研究人员的方法， 并帮助稳定这些方法，以在社区中实现可持续存在。我们也期望 我们在DBP中应用TR＆D技术来产生新的挑战，以帮助推动新的挑战 我们正在进行的TR＆D开发和成熟的各个方面。