Core B - Analytical Core

核心 B - 分析核心

基本信息

批准号：
10225371
负责人：
ANDREW J MORRIS
金额：
$ 19.13万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-01 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10225371
关键词：
Adipose tissue Analytical Chemistry Animals Area Award Biological Assay Biological Markers Blood Cardiovascular Diseases Centers of Research Excellence Clinical Collaborations Complex Mixtures Core Facility Coupled Data Development Diet Diet and Nutrition Dietary Carbohydrates Dietary Fats Disease Ecology Ensure Fees Funding Glucose Goals Grant High Pressure Liquid Chromatography Human Incidence Individual Institution Journals Kentucky Laboratories Link Lipids Malignant Neoplasms Mass Fragmentography Mass Spectrum Analysis Measures Medicine Methods National Institute of Environmental Health Sciences Neurodegenerative Disorders Non-Insulin-Dependent Diabetes Mellitus Obesity Obesity associated disease Peer Review Phase Phospholipids Pre-Clinical Model Publications Reporting Research Research Personnel Research Support Resolution Resources Risk Factors Rural Services Structure Testing Time Tissues Tracer Training and Education Triglycerides United States National Institutes of Health Urine analytical method base biomarker development cardiovascular risk factor cohort college computational platform disorder risk energy balance fatty acid metabolism human disease human subject instrumentation interest lipid metabolism lipidomics metabolic abnormality assessment metabolic phenotype obesity development operation programs small molecule stable isotope western diet

项目摘要

The Phase III Analytical Core will support research into mechanisms linking obesity, lipid metabolism and human disease risk through continued use of facility core instrumentation and resources to develop and support a suite of analytical services tailored for studies of the relationship between diet, obesity and disease mechanisms using preclinical models and clinical approaches. In addition, expansion of services in lipidomics and stable isotope tracer studies will augment capabilities for small animal metabolic phenotyping. This core has developed extensive instrumentation that enables quantitation of a broad range of small molecules, untargeted profiling of lipids in complex mixtures, targeted quantification of biomarkers linking obesity to disease, and methods for studies of glucose and fatty acid metabolism. Each of these analytical capabilities are of direct relevance to studies focused on diet, nutrition, and obesity in disease development. This core has contributed data for more than 110 peer reviewed publications since 2008 in high impact journals, provided services for more than 60 investigators, and contributed to new grant awards in environmental sciences. The core is well equipped, and with expanded services is anticipated to develop self-sustaining capacity in Phase III of the COCVD.

第三阶段分析核心将支持肥胖、脂质代谢和肥胖之间联系机制的研究。通过持续使用设施核心仪器和资源来开发和控制人类疾病风险支持一套专为研究饮食、肥胖和疾病之间关系而定制的分析服务使用临床前模型和临床方法的机制。此外，脂质组学服务的扩展稳定同位素示踪剂研究将增强小动物代谢表型分析的能力。这个核心开发了广泛的仪器，可以对各种小分子进行定量，对复杂混合物中的脂质进行非靶向分析，对与肥胖相关的生物标志物进行靶向定量疾病以及葡萄糖和脂肪酸代谢的研究方法。这些分析能力中的每一个与疾病发展中饮食、营养和肥胖的研究直接相关。该核心有自 2008 年以来，为高影响力期刊上的 110 多篇同行评审出版物提供了数据为 60 多名研究人员提供服务，并为环境科学领域的新拨款做出了贡献。这核心设施齐全，随着服务的扩展，预计将在第一阶段发展自我维持能力 COCVD III。