Effectors of retrotransposon movement

逆转录转座子运动的效应器

基本信息

批准号：
10224748
负责人：
David J. Garfinkel
金额：
$ 44.25万
依托单位：
UNIVERSITY OF GEORGIA
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10224748
关键词：
Address Adopted Affect Affinity Chromatography Binding Biochemical Biochemistry Biological Biological Assay Biology CDK6-associated protein p18 Capsid Capsid Proteins Cells Cellular biology Collaborations Complementary DNA Complex Cryo-electron tomography Cryoelectron Microscopy DNA Polymerase III Data Defective Viruses Defense Mechanisms Dose Elements Enzymes Escape Mutant Eukaryota Evolution GAG Gene Genes Genetic Genome Genomics Goals HIV HIV-1 Integrase Left Long Terminal Repeats Maps Mediating Methods Molecular Chaperones Movement Nature Nuclear Export Nucleic Acids Oncogenic Parasites Peptide Hydrolases Process Production Proteins RNA RNA Polymerase III Reading Frames Recording of previous events Research Research Personnel Resistance Retroelements Retrotransposon Retroviridae Retrovirology Reverse Transcription Role Saccharomyces Saccharomyces cerevisiae Shapes Sheep Site Structure System Transcript Translating Universities Variant Virus Virus Assembly Virus-like particle Work Yeasts base cofactor flexibility genome sciences genomic RNA genomic data information model inhibitor/antagonist insight interdisciplinary approach interest medical schools multidisciplinary new therapeutic target novel particle structural biology three dimensional structure

项目摘要

PROJECT SUMMARY/ABSTRACT Effectors of retrotransposon movement The retrovirus-like transposon Ty1 of Saccharomyces is an informative model for understanding how retrotransposons and retroviruses mobilize, shape the genome, and interact with host and element encoded factors. The additive nature of the retroelement replication cycle can result in massive increases in copy number if left unchecked. Host cells have countered with several defense systems, including domestication of retroelement genes that now act as restriction factors to minimize propagation. We discovered a truncated form of the Ty1 capsid protein that inhibits virus-like particle assembly and function. This self-encoded restriction factor expands the repertoire of defense proteins targeting the capsid. Our work also highlights an intriguing host-parasite strategy. Instead of inhibiting all transposon movement by domesticating the restriction gene as a distinct locus, yeast and Ty1 may have coevolved a relationship that allows high levels of transposition when copy numbers are low and progressively less transposition as copy numbers rise. Our goal is to understand the mechanism and evolution of Ty1 copy number control by a combination of genetic, genomic, cell biological, biochemical and structural approaches.

项目概要/摘要逆转录转座子运动的效应器酵母菌的类逆转录病毒转座子 Ty1 是一个信息模型，用于了解如何逆转录转座子和逆转录病毒动员、塑造基因组，并与宿主和编码元件相互作用因素。逆转录元件复制周期的累加性质可以导致复制量的大量增加如果未选中，则为数字。宿主细胞用多种防御系统进行反击，包括驯化逆转录因子基因现在充当限制因子以最大限度地减少传播。我们发现了一个被截断的 Ty1 衣壳蛋白的形式，抑制病毒样颗粒的组装和功能。这个自编码的限制因子扩展了针对衣壳的防御蛋白的库。我们的工作还强调了有趣的宿主-寄生虫策略。不是通过驯化限制来抑制所有转座子运动基因作为一个独特的基因座，酵母和 Ty1 可能共同进化出一种关系，允许高水平的当拷贝数较低时转座，随着拷贝数增加转座逐渐减少。我们的目标是了解 Ty1 拷贝数通过遗传组合控制的机制和进化，基因组学、细胞生物学、生化和结构方法。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Structure of a Ty1 restriction factor reveals the molecular basis of transposition copy number control.

DOI：
10.1038/s41467-021-25849-0
发表时间：
2021-09-22
期刊：
Nature communications
影响因子：
16.6
作者：
Cottee MA;Beckwith SL;Letham SC;Kim SJ;Young GR;Stoye JP;Garfinkel DJ;Taylor IA
通讯作者：
Taylor IA

Reproducible evaluation of transposable element detectors with McClintock 2 guides accurate inference of Ty insertion patterns in yeast.

DOI：
10.1186/s13100-023-00296-4
发表时间：
2023-07-14
期刊：
MOBILE DNA
影响因子：
4.9
作者：
Chen, Jingxuan;Basting, Preston J.;Han, Shunhua;Garfinkel, David J.;Bergman, Casey M.
通讯作者：
Bergman, Casey M.

Horizontal transfer and recombination fuel Ty4 retrotransposon evolution in Saccharomyces.