UCLA IDDRC: Structural and Functional Visualization Core

加州大学洛杉矶分校 IDDRC：结构和功能可视化核心

基本信息

批准号：
10224913
负责人：
SAMANTHA J BUTLER
金额：
$ 18.45万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10224913
关键词：
3-Dimensional Affect Animal Model Animals Axon Biological Biological Models Biological Process Brain Brain region Cell Culture Techniques Cell physiology Cells Complex Coupled Data Dendrites Developmental Disabilities Disease Fluorescence Microscopy Gene Expression Profiling Generations Genetic Human Image In Vitro Intellectual and Developmental Disabilities Research Centers Laboratories Learning Light Light Microscope Magnetic Resonance Imaging Memory Methodology Methods Microscope Microscopy Modality Modeling Molecular Probes Mus Nervous System Physiology Neurons Optics Organ Organoids Patients Pattern Photons Population Protocols documentation Recording of previous events Research Research Personnel Research Support Resolution Resources Rodent Rodent Model Role Sampling Scientist Services Sleep disturbances Spectrum Analysis Stem Cell Research Structure Techniques Technology Therapeutic Intervention Tissues Translating Visual Acuity Visualization Work animal imaging brain circuitry cognitive neuroscience community center developmental disease fluorescence microscope holistic approach improved in vivo imaging light microscopy member miniaturize nervous system development neural circuit neural implant new technology next generation novel novel imaging technology open source optical imaging optogenetics relating to nervous system stem cells submicron two-photon voucher

项目摘要

CORE E: STRUCTURAL AND FUNCTIONAL VISUALIZATION Samantha Butler, Core Director; Peyman Golshani, Core Co-Director; Neil Harris and Susan Bookheimer; MRI sub-core directors Abstract The Structural and Functional Visualization Core provides comprehensive imaging services to the members of UCLA Intellectual & Developmental Disabilities Research Center (IDDRC), working on any aspect of the genetic and environmentally-induced developmental diseases affecting nervous system development and function. The research ongoing in the UCLA IDDRC spans basic scientists using reductionist approaches to elucidate the mechanisms specific to intellectual developmental disabilities (IDDs) to clinicians assessing therapeutic interventions for patients. To accommodate all of their imaging requirements, we provide access to three light microscopy cores, including a microscopy suite dedicated to IDDRC researchers, human and animal MRI facilities and the technical support needed to initiate and complete any imaging analysis. The Structural and Functional Visualization Core also works to develop new technologies for visualizing biological samples and in turn provide them to IDDRC researchers. In this proposal, we are focused on [1] developing smaller lighter one-photon miniaturized fluorescent microscopes for live imaging neural activity in freely moving animals and [2] refining the methods for CLARITY and iDISCO, protocols that render tissue transparent thereby permitting unparalleled visual acuity into the complex circuitry of the brain. These techniques offer the promise of a holistic approach to imaging, permitting IDDRC researchers to translate mechanism into therapy. For example, researchers investigating a specific intellectual disorder will be able perform MRI on patients to identify the affected region of the brain, implant miniaturized microscopes in rodent models to perform Ca2+ imaging in vivo to examine how the firing patterns of specific populations of neuron are mechanistically altered by the disease, while concomitantly examining putative aberrant circuit formation using light microscopy coupled with CLARITY. Finally, this core also supports the efforts of all the other cores, offering IDDRC researchers the ability to both probe molecular and cellular function at any level from the sub- cellular to living animals and determine the consequence of therapeutic interventions.

核心E：结构和功能可视化核心主任萨曼莎·巴特勒（Samantha Butler）； Peyman Golshani，核心联合导演； Neil Harris和Susan Bookheimer； MRI子核心主管抽象的结构和功能可视化核心为 UCLA知识与发展残疾研究中心（IDDRC）的成员，工作在影响遗传和环境引起的发育疾病的任何方面神经系统的发展和功能。 UCLA IDDRC跨度正在进行的研究基础科学家使用还原主义的方法来阐明智力特定的机制发育障碍（IDDS）给评估患者治疗干预措施的临床医生。到满足他们所有的成像要求，我们提供了三个光学显微镜的访问权限核心，包括专门用于IDDRC研究人员，人类和动物MRI的显微镜套件设施和启动和完成任何成像分析所需的技术支持。结构和功能可视化核心还可以开发新技术为了可视化生物样品，然后将它们提供给IDDRC研究人员。在此提案中，我们专注于[1]开发较小的较小的单光子微型荧光显微镜用于自由移动动物的实时成像神经活动，[2]提出清晰的方法和Idisco，使组织透明的协议从而允许无与伦比的视力进入大脑的复杂电路。这些技术提供了整体方法的希望成像，允许IDDRC研究人员将机制转化为治疗。例如，研究特定智力障碍的研究人员将能够对患者进行MRI 识别大脑的影响区域，将植入物在啮齿动物模型中的微型显微镜植入到在体内执行Ca2+成像，以检查如何在特定神经元的特定种群的点火模式在机械上因疾病而改变，同时检查了假定的异常电路使用光学显微镜形成，结合清晰。最后，该核心还支持所有其他核心的努力，并提供IDDRC 研究人员能够从亚下探测任何水平的分子和细胞功能细胞到活动物，并确定治疗干预的结果。