Structures, Dynamics and Signaling Mechanisms of Modular Photoreceptors

模块化感光器的结构、动力学和信号机制

• 批准号: 10227480
• 负责人: XIAOJING YANG
• 金额: $ 16万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10227480
• 关键词: Administrative Supplement; Allosteric Regulation; Cryoelectron Microscopy; Ensure; Funding; Goals; Light; Methods; Molecular; Molecular Conformation; Photoreceptors; Preparation; Research; Sampling; Signal Transduction; Structure; System; biological systems; experimental study; insight; instrument; parent grant; particle; success

Project Summary The goal of this administrative supplement is to acquire key instruments for setting up an in-house sample preparation system to facilitate dynamic studies of large photoreceptor molecules using the cryoEM single particle analysis method. Leveraging on the progress made in the first two years of this funding period, this system will enable direct observation and analytical deconvolution of light-induced conformational dynamics in photoreceptor particles that cannot be obtained by other methods. This experimental capability will allow controlled photoexcitation of light sensitive systems, and ensure the sample quality and consistency critical to the success of dynamic experiments. It will strengthen our ongoing effort of the parent grant that centers on the mechanistic understanding of light signaling and allosteric regulation in modular photoreceptors.

项目概要 该行政补充文件的目标是获得建立内部样本的关键工具 制备系统，以促进使用冷冻电镜单机对大光感受器分子进行动态研究 粒子分析法。凭借本资助期前两年取得的进展， 系统将能够直接观察和分析光诱导构象动力学的反卷积 存在于光感受器颗粒中，无法通过其他方法获得。这种实验能力将允许 光敏系统的受控光激发，并确保样品质量和一致性至关重要 动态实验的成功。它将加强我们持续努力的家长补助金，重点是 对模块化光感受器中光信号和变构调节的机制理解。

项目成果

Transmembrane Helices Tilt, Bend, Slide, Torque, and Unwind between Functional States of Rhodopsin.

• DOI: 10.1038/srep34129
• 发表时间: 2016-09-23
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Ren Z;Ren PX;Balusu R;Yang X
• 通讯作者: Yang X

Ultrafast Structural Changes Decomposed from Serial Crystallographic Data.

从连续晶体学数据分解的超快结构变化。

• DOI: 10.1021/acs.jpclett.9b02375
• 发表时间: 2019
• 期刊: The journal of physical chemistry letters
• 作者: Ren,Zhong

Light Signaling Mechanism of Two Tandem Bacteriophytochromes.

两种串联细菌光敏色素的光信号机制。

• DOI: 10.1016/j.str.2015.04.022
• 发表时间: 2015
• 期刊: Structure (London, England : 1993)
• 作者: Yang,Xiaojing;Stojković,EminaA;Ozarowski,WesleyB;Kuk,Jane;Davydova,Erna;Moffat,Keith
• 通讯作者: Moffat,Keith

Structure of the response regulator RPA3017 involved in red-light signaling in Rhodopseudomonas palustris.

沼泽红假单胞菌中参与红光信号传导的反应调节器 RPA3017 的结构。

• DOI: 10.1107/s2053230x15014661
• 发表时间: 2015
• 期刊: Acta crystallographica. Section F, Structural biology communications
• 作者: Yang,Xuefei;Zeng,Xiaoli;Moffat,Keith;Yang,Xiaojing
• 通讯作者: Yang,Xiaojing

Crystal-on-crystal chips for in situ serial diffraction at room temperature.

• DOI: 10.1039/c8lc00489g
• 发表时间: 2018-07-24
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: Ren Z;Ayhan M;Bandara S;Bowatte K;Kumarapperuma I;Gunawardana S;Shin H;Wang C;Zeng X;Yang X
• 通讯作者: Yang X