Component for Institution # 264313

机构组件

基本信息

批准号：
10398227
负责人：
Toby K. Eisenstein
金额：
$ 20.17万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-30 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10398227
关键词：
3-Dimensional Alcohols Antibody Formation Area Area Analyses Argon Biological Assay Biological Response Modifiers Blood - brain barrier anatomy Blood specimen Brain Brain region Cannabinoids Cell Culture Techniques Cell Line Cells Cellular Assay Cocaine Collaborations Computer software Dendritic Spines Dependence Detection Drug Regulations Educational process of instructing Enzyme Immunoassay Enzyme-Linked Immunosorbent Assay Event Exposure to Faculty Fluorescence Golgi Apparatus Graft Rejection Grant HIV Infections Histologic Hormones Human Image Image Analysis Imaging Techniques Immune system Immunohistochemistry Immunologics Immunology Immunology procedure In Situ Hybridization In Vitro Individual Inflammation Mediators Institution Investigation Isometric Exercise Laser Scanning Confocal Microscopy Liquid substance Lymphocyte Magnetism Measurement Measures Mediator of activation protein Mentors Methods Mitogens Mixed Lymphocyte Culture Test Molecular Mycoplasma National Institute of Drug Abuse Natural Killer Cells Neurons Nicotine Opioid Organ Transplantation Pharmaceutical Preparations Physiological Plasma Process Protein Array Proteins Proteome RNA Research Research Personnel Resources Rodent Rodent Model Role Running Sampling Serum Skin graft Spleen Stains Substance Use Disorder Substance abuse problem Substance of Abuse Supporting Cell System Techniques Testing Tissue Sample Tissue imaging Tissues Western Blotting Withdrawal Work addiction arm base cathinone cellular imaging chemokine cytokine density drug of abuse high resolution imaging histological image human tissue imaging capabilities imaging system immune imaging instrumentation live cell microscopy lymph nodes microscopic imaging particle psychostimulant ranpirnase receptor expression research and development response theories tissue processing

项目摘要

The Cell and Immunology Core of the NIDA P30 Center provides the expertise and resources for researchers to expand their investigations to include immunological, cellular and histological endpoints. The capability of carrying out immune assays provides a fertile area for collaborations to investigate the potential role of chemokines and cytokines in addictive processes. Analyses can be performed on fluids, tissues, or homogenates obtained from rodents or humans. Many projects supported by the Cell and Immunology Core investigate the effects of the intersection of drugs with HIV infection in rodent models or in human tissue or blood samples. The Core has carried out projects relating to effects of a wide variety of abused substances including opioids, cannabinoids, psychostimulants, nicotine, and alcohol. The Cell and Immunology Core provides expertise in measurement of chemokines, cytokines, and hormones in plasma, homogenized brain or brain substructure tissues, brain dialysate fluid, spleen or lymph node homogenates, and cell culture supernatants using protein arrays, as well as multiplex platforms for simultaneously measuring a panel of mediators, and by ELISA for assay of levels of single immune mediators. The Core also offers a variety of histological and tissue imaging techniques for examining aspects of brain function including receptor expression, microglial activation, dendritic spine density, and blood-brain barrier integrity. Resources available include tissue processing, embedding, sectioning and detection using chromogens and fluorescence (single or multiplex). Advanced techniques offered include tissue clearing (via CLARITY) and special stains such Golgi-cox and Fluro-Jade staining. In addition, the Core offers epifluorescence, confocal, intravital and live cell microscopy. Image analysis for area, particle analysis, tracking and 3D volumetric measurements can be performed using dedicated workstations configured to run NIS elements AR (Nikon) and Imaris (Bitplane). The Core has available the in-situ hybridization platform from RNAscope to detect RNA in tissues. The Core also carries out functional immune assays such as the Mixed Lymphocyte Reaction (MLR), an in vitro correlate of organ and skin graft rejection, response of lymphocytes to mitogens, Natural Killer cell activity, and in vitro antibody formation. The Core screens cell lines for those doing research in substance abuse for mycoplasma contamination. The Core is committed to mentoring trainees and faculty new to immunological assays and imaging analysis by teaching them the theory behind various assays and instructing them in how to carry out the techniques.

NIDA P30 中心的细胞和免疫学核心为以下领域提供专业知识和资源：研究人员将他们的研究范围扩大到包括免疫学、细胞和组织学终点。进行免疫测定的能力为合作研究提供了肥沃的领域趋化因子和细胞因子在成瘾过程中的潜在作用。可以对液体进行分析，从啮齿动物或人类获得的组织或匀浆。许多项目得到了 Cell 和免疫学核心研究药物与啮齿动物模型中 HIV 感染的交叉影响或在人体组织或血液样本中。核心已开展了与广泛影响相关的项目各种滥用物质，包括阿片类药物、大麻素、精神兴奋剂、尼古丁和酒精。细胞和免疫学核心提供趋化因子、细胞因子和细胞因子测量方面的专业知识血浆、均质脑或脑下部结构组织、脑透析液、脾脏或使用蛋白质阵列以及多重分析获得淋巴结匀浆和细胞培养上清液用于同时测量一组介质的平台，并通过 ELISA 测定单个介质的水平免疫介质。该核心还提供各种组织学和组织成像技术检查大脑功能的各个方面，包括受体表达、小胶质细胞激活、树突棘密度和血脑屏障完整性。可用资源包括组织处理、包埋、使用显色剂和荧光（单一或多重）进行切片和检测。先进技术提供的服务包括组织透明化（通过 CLARITY）和特殊染色剂，例如 Golgi-cox 和 Fluro-Jade 染色。此外，Core 还提供落射荧光、共聚焦、活体和活细胞显微镜检查。图像分析对于面积、颗粒分析、跟踪和 3D 体积测量，可以使用专用的配置为运行 NIS elements AR (Nikon) 和 Imaris (Bitplane) 的工作站。核心已经可用 RNAscope 的原位杂交平台可检测组织中的 RNA。核心还执行功能性免疫测定，例如混合淋巴细胞反应 (MLR)，这是器官的体外相关性和皮肤移植排斥、淋巴细胞对有丝分裂原的反应、自然杀伤细胞活性和体外抗体形成。核心为从事药物滥用研究的人员筛选细胞系支原体污染。核心致力于指导新来的学员和教师通过教他们各种测定背后的理论和免疫学测定和成像分析指导他们如何实施这些技术。