Animal Model/Pathology Core

动物模型/病理学核心

基本信息

批准号：
10397672
负责人：
Igor Morozov
金额：
$ 26.24万
依托单位：
KANSAS STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10397672
关键词：
Animal Experimentation Animal Experiments Animal Model Animals Antibodies Area Autopsy Award Biomedical Research Caring Centers of Research Excellence Communicable Diseases Consultations Containment Core Facility Development Diagnostic Diagnostic tests Ensure Equipment Evaluation Experimental Designs Experimental Pathology Extramural Activities Funding Goals Hybridomas IACUC Immune Sera Individual Infrastructure Investigation Kansas Medicine Methodology Modeling Molecular and Cellular Biology Pathologic Pathologist Pathology Performance Pilot Projects Process Protocols documentation Reagent Research Research Design Research Infrastructure Research Institute Research Personnel Research Project Grants Reservations Rodent Sampling Schedule Seeds Services System Testing Time TimeLine Tissue Banks Tissue Sample Training Universities Vertebrates Veterinary Medicine Work Zoonoses animal facility biosafety level 3 facility biosecurity college comparative experimental study improved instrument laser capture microdissection pathogen sample collection single-cell RNA sequencing success synergism

项目摘要

Project Summary: The main goal of the Animal Model/Pathology (AMP) Core is to provide expert support for animal research within the Center on Emerging and Zoonotic Infectious Diseases (EZID), including the selection of appropriate models, biosafety and IACUC compliance submissions, and advanced, personalized pathology services for a variety of species. The AMP Core will oversee and support all animal experimentation and post experimental pathology evaluations performed by EZID project leaders (PLs). Animal experiments will be conducted in Kansas State University (KSU) animal facilities located in Coles Hall in the College of Veterinary Medicine (CVM) and at the Biosecurity Research Institute (BRI). Vivarium rooms at Coles Hall provide the capability to conduct research with rodents and other small vertebrates in BSL-2 containment. Animal research space at the BRI includes BSL-3Ag animal rooms and an ABSL-3 vivarium. BRI facilities allow work with select agents, including zoonotic pathogens for animal experiments at BSL-3 level of containment. The main focus of the AMP Core will be supporting on-going primary and pilot/seed projects of the EZID COBRE Center involving animal studies and pathology analysis. All support will be personalized and project-specific with the focus to satisfy specific animal research needs of the individual EZID PLs. The AMP Core will provide assistance and expertise in appropriate study designs and in preparing and obtaining IACUC approval. The Core will also coordinate with the Comparative Medicine Group (CMG; responsible for animal and veterinary care) and BRI timelines and space allocation. The AMP Core will oversee and support the execution of animal studies conducted by the Center's primary and pilot/seed PLs and their teams. The Core will also develop and conduct pilot studies on development of challenge models in rodents and other small animals essential for EZID Center projects. For pathological evaluations, the AMP Core will utilize expertise and equipment available in the Department of Diagnostic Medicine/Pathobiology. All pathologic evaluations, tissue collections, and testing will be done under the oversight of the AMP Core Associate Director, a board-certified pathologist. Existing infrastructure will be enhanced by adding new equipment and services to expand capabilities of the Core and ensure its sustainability. This includes the acquisition of a laser capture microdissection instrument which will allow significant synergy between the two COBRE cores and for the first time at KSU the performance of single-cell RNA-Seq experiments. The AMP Core will also produce hybridomas and hyper-immune sera for the development of diagnostic tests essential to COBRE research projects. This work will be done in coordination with the Molecular and Cellular Biology Core to ensure that appropriate sample testing methodology is developed and available for testing of the generated antibodies. Successful implementation of the AMP Core will improve the biomedical research infrastructure within EZID and at KSU and significantly contribute to the overall COBRE objectives.

项目概要：动物模型/病理学 (AMP) 核心的主要目标是为动物研究提供专家支持新发和人畜共患传染病中心（EZID），包括选择适当的模型，生物安全和 IACUC 合规性提交，以及针对各种疾病的先进、个性化病理学服务物种。 AMP 核心将监督和支持所有动物实验和实验后由 EZID 项目负责人 (PL) 进行的病理学评估。动物实验将在堪萨斯州立大学 (KSU) 动物设施位于兽医学院 (CVM) 的 Coles Hall 以及生物安全研究所（BRI）。 Coles Hall 的动物园室可以进行 BSL-2 遏制中的啮齿动物和其他小型脊椎动物的研究。 BRI 的动物研究空间包括 BSL-3Ag 动物室和 ABSL-3 动物室。 BRI 设施允许与精选代理商合作，包括用于动物实验的人畜共患病原体 BSL-3 防护等级。 AMP 核心的主要焦点将是支持 EZID COBRE 中心正在进行的主要和试点/种子项目，涉及动物研究和病理分析。所有支持都将是个性化的、针对特定项目的，重点是满足特定动物的需求各个 EZID PL 的研究需求。 AMP 核心将在适当的方面提供帮助和专业知识研究设计以及准备和获得 IACUC 批准。核心还将与比较医学小组（CMG；负责动物和兽医护理）和 BRI 的时间表和空间分配。 AMP 核心将监督和支持该中心进行的动物研究的执行主要和试点/种子 PL 及其团队。核心还将开展发展试点研究对 EZID 中心项目至关重要的啮齿动物和其他小动物的挑战模型。对于病理性评估后，AMP 核心将利用诊断部门提供的专业知识和设备医学/病理学。所有病理评估、组织采集和检测都将在监督下进行 AMP 核心副主任，委员会认证的病理学家。现有基础设施将得到加强添加新设备和服务以扩展核心的能力并确保其可持续性。这包括购买激光捕获显微切割仪器，这将使两者之间产生显着的协同作用 COBRE 核心并首次在 KSU 进行单细胞 RNA 测序实验。 AMP 核心还将生产杂交瘤和超免疫血清，用于开发必要的诊断测试 COBRE 研究项目。这项工作将与分子和细胞生物学核心协调完成确保开发适当的样本测试方法并可用于测试生成的抗体。 AMP核心的成功实施将改善生物医学研究基础设施在 EZID 和 KSU 内，并对 COBRE 的总体目标做出了重大贡献。