Animal Model/Pathology Core

动物模型/病理学核心

• 批准号: 10397672
• 负责人: Igor Morozov
• 金额: $ 26.24万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10397672
• 关键词: Animal Experimentation; Animal Experiments; Animal Model; Animals; Antibodies; Area; Autopsy; Award; Biomedical Research; Caring; Centers of Research Excellence; Communicable Diseases; Consultations; Containment; Core Facility; Development; Diagnostic; Diagnostic tests; Ensure; Equipment; Evaluation; Experimental Designs; Experimental Pathology; Extramural Activities; Funding; Goals; Hybridomas; IACUC; Immune Sera; Individual; Infrastructure; Investigation; Kansas; Medicine; Methodology; Modeling; Molecular and Cellular Biology; Pathologic; Pathologist; Pathology; Performance; Pilot Projects; Process; Protocols documentation; Reagent; Research; Research Design; Research Infrastructure; Research Institute; Research Personnel; Research Project Grants; Reservations; Rodent; Sampling; Schedule; Seeds; Services; System; Testing; Time; TimeLine; Tissue Banks; Tissue Sample; Training; Universities; Vertebrates; Veterinary Medicine; Work; Zoonoses; animal facility; biosafety level 3 facility; biosecurity; college; comparative; experimental study; improved; instrument; laser capture microdissection; pathogen; sample collection; single-cell RNA sequencing; success; synergism

Project Summary: The main goal of the Animal Model/Pathology (AMP) Core is to provide expert support for animal research within the Center on Emerging and Zoonotic Infectious Diseases (EZID), including the selection of appropriate models, biosafety and IACUC compliance submissions, and advanced, personalized pathology services for a variety of species. The AMP Core will oversee and support all animal experimentation and post experimental pathology evaluations performed by EZID project leaders (PLs). Animal experiments will be conducted in Kansas State University (KSU) animal facilities located in Coles Hall in the College of Veterinary Medicine (CVM) and at the Biosecurity Research Institute (BRI). Vivarium rooms at Coles Hall provide the capability to conduct research with rodents and other small vertebrates in BSL-2 containment. Animal research space at the BRI includes BSL-3Ag animal rooms and an ABSL-3 vivarium. BRI facilities allow work with select agents, including zoonotic pathogens for animal experiments at BSL-3 level of containment. The main focus of the AMP Core will be supporting on-going primary and pilot/seed projects of the EZID COBRE Center involving animal studies and pathology analysis. All support will be personalized and project-specific with the focus to satisfy specific animal research needs of the individual EZID PLs. The AMP Core will provide assistance and expertise in appropriate study designs and in preparing and obtaining IACUC approval. The Core will also coordinate with the Comparative Medicine Group (CMG; responsible for animal and veterinary care) and BRI timelines and space allocation. The AMP Core will oversee and support the execution of animal studies conducted by the Center's primary and pilot/seed PLs and their teams. The Core will also develop and conduct pilot studies on development of challenge models in rodents and other small animals essential for EZID Center projects. For pathological evaluations, the AMP Core will utilize expertise and equipment available in the Department of Diagnostic Medicine/Pathobiology. All pathologic evaluations, tissue collections, and testing will be done under the oversight of the AMP Core Associate Director, a board-certified pathologist. Existing infrastructure will be enhanced by adding new equipment and services to expand capabilities of the Core and ensure its sustainability. This includes the acquisition of a laser capture microdissection instrument which will allow significant synergy between the two COBRE cores and for the first time at KSU the performance of single-cell RNA-Seq experiments. The AMP Core will also produce hybridomas and hyper-immune sera for the development of diagnostic tests essential to COBRE research projects. This work will be done in coordination with the Molecular and Cellular Biology Core to ensure that appropriate sample testing methodology is developed and available for testing of the generated antibodies. Successful implementation of the AMP Core will improve the biomedical research infrastructure within EZID and at KSU and significantly contribute to the overall COBRE objectives.

项目摘要： 动物模型/病理（AMP）核心的主要目标是为动物研究提供专家支持 新兴和人畜共动性传染病（Ezid）的中心，包括选择合适的模型， 生物安全和IACUC合规性提交以及高级，个性化的病理学服务 物种。 AMP核心将监督和支持所有动物实验并在实验后 EZID项目领导者（PLS）进行的病理评估。动物实验将在 堪萨斯州立大学（KSU）的动物设施位于兽医学院（CVM）的Coles Hall 以及生物安全研究所（BRI）。 Coles Hall的Vivarium Room提供了行为的能力 BSL-2遏制中使用啮齿动物和其他小脊椎动物进行研究。 BRI的动物研究空间 包括BSL-3AG动物室和Absl-3 Vivarium。 BRI设施允许与精选代理一起工作，包括 在BSL-3遏制水平上进行动物实验的人畜共患病原体。 AMP核心的主要重点将 支持涉及动物研究和 病理分析。所有支持都将是个性化的，并且专注于项目以满足特定动物的重点 个体的研究需求。 AMP核心将提供适当的帮助和专业知识 研究设计以及准备和获得IACUC批准。核心还将与 比较医学组（CMG；负责动物和兽医护理）以及BRI时间表以及空间 分配。 AMP核心将监督和支持该中心进行的动物研究的执行 初级和飞行员/种子PLS及其团队。核心还将开发和进行开发试点研究 啮齿动物和其他小动物中的挑战模型，对度中心项目必不可少。用于病理 评估，AMP核心将利用诊断部门可用的专业知识和设备 医学/病理学。所有病理评估，组织收集和测试将在监督下进行 AMP核心副主任，董事会认证的病理学家。现有的基础设施将通过 添加新的设备和服务以扩大核心的能力并确保其可持续性。这包括 获得激光捕获的微解剖仪器，这将允许两者之间的显着协同作用 脚底岩心，第一次在KSU上进行单细胞RNA-Seq实验的性能。放大器核心 还将产生杂交瘤和超免疫血清，以开发对诊断测试的开发 毛病研究项目。这项工作将与分子和细胞生物学核心协调完成 确保开发适当的样品测试方法并可用于测试生成的 抗体。成功实施AMP核心将改善生物医学研究基础设施 在Ezid和KSU内，并显着促进了整个鞋底目标。