Ectonucleotidases in ischemic heart disease

外切核苷酸酶在缺血性心脏病中的作用

基本信息

批准号：
10213112
负责人：
Richard J Gumina
金额：
$ 38.42万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-07-14 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10213112
关键词：
Acute myocardial infarction Address Adenosine Adenosine Monophosphate Anti-Inflammatory Agents Attenuated Bone Marrow Cardiac Cardiovascular Diseases Cardiovascular Pathology Cells Cicatrix Data Enzymes Equilibrium Extracellular Matrix Fibroblasts Fibrosis Funding Goals Heart Heart Diseases Heart failure Hydrolysis Image In Vitro Inflammation Inflammatory Inflammatory Response Integral Membrane Protein Interleukin-4 Kidney Knock-out Lead Liver Lung Mass Spectrum Analysis Measurable Mediating Mediator of activation protein Metabolic Pathway Molecular Myocardial Infarction Myocardial Ischemia Myocardial rupture Nucleotides Outcome Study P2X-receptor Pathway interactions Patients Phenotype Process Purinergic P1 Receptors Purinergic P2 Receptors Purinoceptor Receptor Activation Reperfusion Injury Role STEM research Signal Pathway Signal Transduction Skin Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Testing Thrombosis United States Up-Regulation Work autocrine base biosignature cardiac repair coronary fibrosis extracellular healing in vivo macrophage myocardial damage novel novel therapeutic intervention novel therapeutics paracrine prevent repaired response restraint translational impact tripolyphosphate

项目摘要

More than 1 million people in the United States suffer a myocardial infarction (MI) each year. Myocardial infarction (MI) evokes an intense inflammatory response. Paracrine and autocrine released extracellular ATP (eATP), signaling through purinergic P2 receptors (P2X and P2Y), is a potent modulator of macrophage and fibroblast function. Extracellular ATP is hydrolyzed by the transmembrane protein Ectonucleoside triphosphate diphosphohydrolase 1 (CD39) to yield adenosine monophosphate (AMP), thereby halting pro-inflammatory ATP-mediated signaling. This goal of the project is to define the role of the macrophage and fibroblast- expressed CD39 on post-MI cardiac repair. To accomplish this goal we propose the following: Aim 1: To determine whether CD39 up-regulation is a protective mechanism that constrains autocrine ATP-driven inflammation, preventing exaggerated macrophage responses, and protecting from adverse post-MI fibrosis we will: 1) determine how CD39 activity impacts TLR-4-dependent and IL-4-dependent macrophage functions in vitro, 2) determine the impact of CD39 activity on the in vivo macrophage phenotypic transition from inflammatory to profibrotic functions during post-MI repair, and 3) Determine the role of macrophage CD39 on post-MI repair fibrosis in vivo. Aim 2: To dissect the purinergic pathways involved in CD39-mediated restraint of TGF-β1 activation of cardiac fibroblasts and determine the role of CD39 upregulation in modulating fibroblast function and regulating cardiac repair following MI we will 1) determine the downstream targets through which CD39 attenuates TGF-β1-mediated cardiac fibroblast inflammatory and fibrotic responses, 2) determine the impact of CD39 on the in vivo cardiac fibroblast phenotype during cardiac repair and 3) determine the impact of fibroblast CD39 on in vivo extracellular matrix remodeling during post-MI repair. Secondary Aim: To determine the impact of CD39 expression extracellular matrix remodeling we will use state-of-the-art MALDI-imaging mass spectroscopy to define the early and late biosignatures of cardiac ECM remodeling post-MI and determine the impact of CD39 on ECM remodeling. The outcomes of these studies will reveal the fundamental pathways by which CD39 regulates post-MI myocardial repair and could allow novel therapeutic approaches not only to treat fibrotic disorders of the heart, but also of the skin, lungs, bone marrow, liver, or kidneys, thereby providing an important translational impact.

每年，美国有超过100万人遭受心肌梗塞（MI）。心肌梗塞（MI）引起了强烈的炎症反应。旁分泌和自分泌释放了细胞外ATP （EATP）通过嘌呤能P2受体（P2X和P2Y）信号传导，是巨噬细胞的潜在调节剂成纤维细胞功能。细胞外ATP被跨膜蛋白纤维蛋白三磷酸水解水解双磷酸化酶1（CD39）产生腺苷一磷酸（AMP），从而停止促炎性炎症 ATP介导的信号传导。该项目的目标是定义巨噬细胞和成纤维细胞的作用在MI后心脏修复上表达CD39。为了实现这一目标，我们提出以下建议：目标1：确定CD39上调是否是限制自分泌驱动的受保护机制炎症，防止夸张的巨噬细胞反应并保护不良后MI纤维化，我们意志：1）确定CD39活动如何影响TLR-4依赖性和IL-4依赖性巨噬细胞功能体外，2）确定CD39活性对从体内巨噬细胞表型转变的影响在MI后修复期间对纤维化功能的炎症，3）确定巨噬细胞CD39在体内MI修复纤维化。目标2：剖析CD39介导的约束的嘌呤能途径心脏成纤维细胞的TGF-β1激活，并确定CD39上调在调节成纤维细胞中的作用功能和调节心脏修复后，我们将1）确定下游目标 CD39减弱了TGF-β1介导的心脏成纤维细胞炎症和纤维化反应，2）确定 CD39对心脏修复期间体内心脏成纤维细胞表型的影响，3）确定 MI修复期间的体内细胞外基质重塑上的成纤维细胞CD39。次要目标：确定 CD39表达细胞外基质重塑的影响我们将使用最先进的Maldimimigaging 质谱法定义了MI后心脏ECM重塑的早期和晚期生物签名和确定CD39对ECM重塑的影响。这些研究的结果将揭示基本的 CD39调节MI后心肌修复的途径，可以允许新型治疗方法不仅要治疗心脏的纤维化疾病，还可以治疗皮肤，肺，骨髓，肝脏或肾脏从而提供重要的翻译影响。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Estrogenic bias in T-Lymphocyte biology: Implications for cardiovascular disease.

DOI：
10.1016/j.phrs.2021.105606
发表时间：
2021-08
期刊：
Pharmacological research
影响因子：
9.3
作者：
Rosenzweig R;Gupta S;Kumar V;Gumina RJ;Bansal SS
通讯作者：
Bansal SS

Immune cell Dilemma in Ischemic Cardiomyopathy: To Heal or Not to Heal.

DOI：
10.1016/j.cophys.2020.09.002
发表时间：
2021-03
期刊：
Current opinion in physiology
影响因子：
2.5
作者：
Nehra S;Gumina RJ;Bansal SS
通讯作者：
Bansal SS

Genotype-Guided Use of P2Y12 Inhibitors: A Review of Current State of the Art.

DOI：
10.3389/fcvm.2022.850028
发表时间：
2022
期刊：
Frontiers in cardiovascular medicine
影响因子：
3.6
作者：
Al-Abcha A;Radwan Y;Blais D;Mazzaferri EL Jr;Boudoulas KD;Essa EM;Gumina RJ
通讯作者：
Gumina RJ

Neutralization of SARS-CoV-2 Variants of Concern Harboring Q677H.

DOI：
10.1128/mbio.02510-21
发表时间：
2021-10-26
期刊：
mBio
影响因子：
6.4
作者：
Zeng C;Evans JP;Faraone JN;Qu P;Zheng YM;Saif L;Oltz EM;Lozanski G;Gumina RJ;Liu SL
通讯作者：
Liu SL

Neutralizing antibody responses elicited by SARS-CoV-2 mRNA vaccination wane over time and are boosted by breakthrough infection.