Stanford Tissue Mapping Center

斯坦福大学组织绘图中心

基本信息

批准号：
10213803
负责人：
William James Greenleaf
金额：
$ 109.87万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-19 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10213803
关键词：
3-Dimensional ATAC-seq Antibodies Architecture Attention Behavior Biological Assay Brain Brain Death Cells Cellular Assay Cellular Structures Chromatin Colon Complex Consent Coupled Data Development Dietary Fats Disease Epithelial Epithelial Cells Family Gastrointestinal tract structure Gene Expression Geography Human Immune Immune system Individual Inflammatory Bowel Diseases Informed Consent Intestines Investigation Large Intestine Lead Length Maps Metabolism Methods Molecular Morphology Mucous Membrane Muscle Neurophysiology - biologic function Organ Organ Donor Organ Preservation Pacemakers Phenotype Physiology Population Protocols documentation Regulator Genes Research Resolution Resources Role Signaling Molecule Small Intestines Structure Surface Tissue Preservation Tissues Transplantation Volatile Fatty Acids base body system brain tissue cell type draining lymph node gastrointestinal genome-wide gut microbiota human tissue ileum imaging approach immunoregulation lymphatic circulation microbiome microbiota molecular dynamics multiplexed imaging nutrient absorption programs relating to nervous system single-cell RNA sequencing spatial relationship transcriptome

项目摘要

ABSTRACT: Organ Specific Project Beyond nutrient absorption, the gastrointestinal tract has wide-ranging effects on the normal and diseased physiologies of other organ systems, including metabolism, neural function, and the immune system. A high-resolution map of the bowel would be an invaluable resource to understand normal bowel function and the perturbations that lead to disease. We propose to create this map along the length of the small bowel and colon, both of which have nuanced geographic specializations of function. Using single cell RNA-seq and single cell ATAC-seq, we will profile the gene expression and regulatory programs that define the complex cell populations that drive bowel function. We will use CODEX, a highly-multiplexed, antibody-based mapping method, to define the spatial relationships of these cell populations. These investigations will be performed on tissues that are preserved and procured in a manner suitable for human bowel transplantation. Are target milestone is to characterize the small bowel and colon tissues from a total of 22 individuals over the course of this four-year effort. Our protocol to obtain tissues from human organ donors whose families have provided broad, open access consent is in place, and we are actively collecting tissues for other studies.

摘要：器官特定项目除了营养吸收之外，胃肠道对正常的消化系统也有广泛的影响。以及其他器官系统的患病生理机能，包括新陈代谢、神经功能和免疫系统。高分辨率的肠道图将是宝贵的资源了解正常的肠道功能和导致疾病的干扰。我们建议沿着小肠和结肠的长度创建这张地图，两者都有细微差别功能的地理专业化。使用单细胞 RNA-seq 和单细胞 ATAC-seq，我们将分析定义复杂细胞的基因表达和调控程序驱动肠道功能的人群。我们将使用 CODEX，一种高度多重的、基于抗体的绘图方法，定义这些细胞群的空间关系。这些将对以某种方式保存和采购的组织进行调查适合人体肠移植。目标里程碑是表征小肠吗在这四年的努力中，总共收集了 22 个人的结肠组织。我们的从家属提供广泛、开放的人体器官捐献者那里获取组织的协议获取同意已经到位，我们正在积极收集组织用于其他研究。