Pathology Core

病理学核心

基本信息

批准号：
8940155
负责人：
Robert Adelstein
金额：
$ 89.46万
依托单位：
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8940155
关键词：
American Antibodies Antigens Calcium Chemicals Chemistry Consultations Core Facility Diagnosis Diagnostic Diagnostic Procedure District of Columbia Embryo Equipment Evaluation Experimental Animal Model Experimental Designs Formalin Freezing Frozen Sections Glass Head Hematoxylin and Eosin Staining Method Histocytochemistry Histology Histopathology Image Immune Immunohistochemistry Institutes Iron Laboratories Light Microscope Manuscripts Methods Microscopic Microscopy Microtome - medical device National Heart, Lung, and Blood Institute Organ Paraffin Paraffin Embedding Paraffin Tissue Pathology Pathology Report Periodic acid Schiff stain method Preparation Procedures Process Protocols documentation Publications Registries Reporting Research Project Grants Routine Histology Staining Method Sampling Scientist Services Site Slice Slide Special Histology Staining Method Staining method Stains System Testing Thick Tissue Embedding Tissues Training Trees Work arm digital microwave electromagnetic radiation oil red O programs sample collection tissue fixing tissue processing

项目摘要

1. Overview The NHLBI Pathology Core Facility provides a full range of histopathological services for the DIR program. Our services include histology tissue procedures, diagnostic histo-pathology evaluation and training for histology and immuno-histo-chemistry procedures. Core Services: 1) The main services of the Pathology Core are histology procedures, such as formalin-fixed tissue embedding, sectioning and histology staining. For this purpose, the Core receives samples in fixed or in fresh condition. These samples are processed and embedded in paraffin or OCT component for either paraffin or frozen sections. The paraffin embedded samples can be cut routinely as 5 micron thick sections mounted on glass slides. The serial sections through an entire organ or embryo, or mixed with thick (20-100 microns) and thin (5-10 microns) step sections also can be cut upon request. Frozen sections are normally cut at a thickness of 8-10 microns. The Pathology core provides routine histology staining with Hematoxylin and Eosin according to Harris methods (Laboratory Methods in Histotechnology. Armed Forces Institute of Pathology, Edited by Prophet EB, Mills B, Arrington JB and Sobin LH, pp 56, American Registry of Pathology, Washington, DC, 1992). In addition, we also perform special histology staining, including PAS, Masson tri-chrome, Movat, Elastic Van Gieson and Oil-Red O stain. All these services are performed by the Core staff and finished in a timely manner. Most requests are completed within a two-week period, except urgent requests that can be arranged as a higher-priority request. In some cases, results can be returned to users the next day. 2) Diagnostic pathology. For this service, Core head is involved in discussion of experimental design, sample collection methods and final evaluations of the results. We provide histology products such as cut and stained histology slides, perform immune-staining procedures, evaluate the results, describe the findings and make a scoring sheet with representative photographs. In most cases, this service will contribute to manuscript submission and we will provide final microscopic figures. 3) Training, consultation and trouble shooting for DIR scientists and fellows in the fields of histo-pathology and immuno-histochemistry. We provide training for tissue processing, embedding and sectioning for paraffin and frozen samples. In addition, we also offer testing for antibodies and for immuno-staining procedures and consultation for immuno-staining problems. 2. Operational analysis Since our last report, the pathology core had total of 285 requests for the services listed above. Core users include 58 fellows/doctors from 27 PIs/ laboratories. We have processed a total of 2,747 tissue blocks and cut 22,865 histology slides, which are usually mounted with 2 or 3 tissue sections. Among them, there were 4,301 slides stained with Hematoxylin and Eosin for routine histology. In addition, we also performed 1,250 slides for other specific staining procedures as mentioned above. Detailed lists of user and services are shown in the attached PDF file, which is generated by iLab system. The quantity of table showed included all slides, blocks and staining. Core head is involved in the services for histo-pathological analysis, and immunostaining on research projects for 11 scientists from 6 PI/laboratories including histo-pathological diagnosis, histology evaluations with the score system and provided final images for publications. These resulted in 10 publications in 2013-2014. The Pathology Core offers training for cutting frozen/paraffin sections, immunohistochemistry procedures and histology microphotograph by using Core bright field microscopy equipped digital camera. Core head worked closely with the scientists for selection of antibodies and protocols, and then provided hands-on staining demonstration until the scientist can perform the procedures independently. Currently, we have 18 users for Leica DMB-4000 microscopy and 5 for Cryo-stat (Frozen sections)

1. 概述 NHLBI 病理学核心设施为 DIR 计划提供全方位的组织病理学服务。我们的服务包括组织学组织程序、诊断组织病理学评估以及组织学和免疫组织化学程序培训。核心服务： 1) 病理学核心的主要服务是组织学程序，例如福尔马林固定组织包埋、切片和组织学染色。为此，Core 接收固定或新鲜状态的样品。这些样品经过处理并包埋在石蜡或 OCT 组件中，用于石蜡或冷冻切片。石蜡包埋的样品可以常规切割为 5 微米厚的切片，安装在载玻片上。也可以根据要求切割整个器官或胚胎的连续切片，或混合厚（20-100 微米）和薄（5-10 微米）阶梯切片。冰冻切片通常切成 8-10 微米的厚度。病理学核心根据 Harris 方法提供苏木精和曙红的常规组织学染色（组织技术实验室方法。武装部队病理学研究所，由 Prophet EB、Mills B、Arrington JB 和 Sobin LH 编辑，第 56 页，美国病理学登记处，华盛顿，华盛顿特区，1992）。此外，我们还进行特殊的组织学染色，包括PAS、Masson三色、Movat、Elastic Van Gieson和油红O染色。所有这些服务均由核心人员执行并及时完成。大多数请求都会在两周内完成，但可以安排为更高优先级请求的紧急请求除外。在某些情况下，结果可以在第二天返回给用户。 2）诊断病理学。对于这项服务，核心负责人参与讨论实验设计、样本采集方法和结果的最终评估。我们提供组织学产品，例如切割和染色的组织学载玻片、执行免疫染色程序、评估结果、描述结果并制作带有代表性照片的评分表。在大多数情况下，这项服务将有助于稿件提交，我们将提供最终的微观数据。 3) 为组织病理学和免疫组织化学领域的 DIR 科学家和研究员提供培训、咨询和故障排除。我们提供石蜡和冷冻样本的组织处理、包埋和切片培训。此外，我们还提供抗体检测和免疫染色程序以及免疫染色问题的咨询。 2、运营分析自我们上次报告以来，病理学核心总共收到了 285 个对上述服务的请求。核心用户包括来自 27 个 PI/实验室的 58 名研究员/博士。我们总共处理了 2,747 个组织块并切割了 22,865 个组织学载玻片，这些载玻片通常装有 2 或 3 个组织切片。其中，有4,301张载玻片用苏木精和曙红染色进行常规组织学检查。此外，我们还对 1,250 张载玻片进行了上述其他特定染色程序。所附的 PDF 文件显示了详细的用户和服务列表，该文件由 iLab 系统生成。表中显示的数量包括所有载玻片、块和染色。核心负责人参与为来自 6 个 PI/实验室的 11 名科学家的研究项目提供组织病理学分析和免疫染色服务，包括组织病理学诊断、使用评分系统进行组织学评估，并为出版物提供最终图像。这些成果在 2013 年至 2014 年期间发表了 10 篇出版物。病理学核心提供使用配备核心明视场显微镜的数码相机切割冷冻/石蜡切片、免疫组织化学程序和组织学显微照片的培训。核心负责人与科学家密切合作，选择抗体和方案，然后提供动手染色演示，直到科学家能够独立执行程序。目前，我们有 18 位 Leica DMB-4000 显微镜用户和 5 位冷冻切片用户